Detection of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>in Retail Cheeses from Greece and the Czech Republic

Ikonomopoulos, John; Pavlík, I.; Bartoš, Milan; Svastova, Petra; Ayele, W. Yayo; Roubal, Petr; Lukas, John C.; Cook, Nigel; Gazouli, Maria

doi:10.1128/aem.71.12.8934-8936.2005

Cited by 82 publications

(71 citation statements)

References 19 publications

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“…Over the past few years sufficient information has been generated on the culture of live MAP bacilli from milk of clinically and sub-clinically infected cows and other domestic livestock (Ellingson et al, 2005;Ayele et al, 2005). Recent surveys have reported presence of MAP in commercial supplies of pasteurized retail milk globally (Grant et al, 2002;Ellingson et al, 2005;Gill et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Crohn"s Disease (CD), a systemic disorder in humans where principal clinico-pathological manifestation is the chronic inflammation of the intestines. Increasing evidences suggest that Crohn's disease has an infectious etiology and MAP being the most plausible candidate since it has been frequently reported from commercial milk and milk products globally (Grant et al, 2002;Ikonomopoulos et al, 2005;Ellingson et al, 2005;Ayele et al, 2005;Paolicchi et al, 2012) and has also been isolated from patients suffering from CD (Singh et al, 2008;Naser et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Singh¹,

Stephen²,

Singh³

et al. 2016

JEBAS

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In the present study, Indirect Fluorescent antibody test (iFAT) has been used as the "screening test" for the detection of MAP bacilli in the milk samples of lactating domestic livestock. A total of 372 milk samples from lactating animals were screened by iFAT and results were compared with microscopy of milk samples and milk ELISA test. Comparative analysis of the results of three tests showed that iFAT had fairly good sensitivity (84.7%) and specificity (90.4%), with respect to ZN staining (microscopy) with kappa value of 0.735 and "good" strength of agreement. Similar comparison with milk ELISA test revealed, sensitivity (73.3%) and specificity of (72.6%) with kappa value of 0.443 and strength of agreement was moderate. Lower or higher difference in sensitivity and specificity of iFAT with respect to ZN staining and milk ELISA may be due to the difference in detection "target" of the test i.e., antigen or antibody. It was concluded that iFAT was a reliable and sensitive diagnostic test for the detection of MAP in milk and can also be used for the "mass screening" of the milk samples.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Singh¹,

Stephen²,

Singh³

et al. 2016

JEBAS

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“…Goat's tank-milk and ewe's tank-milk samples were also PCR-positive for the IS900 (23.0 and 23.8%, respectively), providing presumptive evidence for the presence of MAP in Switzerland (Muehlherr et al, 2003). MAP has been cultivated from cheese (Mason et al, 1997;Donaghy et al, 2004;Stabel and Lambertz, 2004;Ikonomopoulos et al, 2005) as well.…”

mentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved

Hruška¹,

Bartoš²,

Králík³

et al. 2005

Vet. Med. - Czech

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Fifty one products of dried milk baby food purchased from 10 producers from seven countries available on the Czech market have been tested. IS900, the specific fragments for Mycobacterium avium subsp. paratuberculosis (MAP) have been detected using PCR in 25 samples (49.0 %) and fragment f57 by real time PCR in 18 samples (35.3%). These results correspond to the epidemiological situation in Europe and are not unexpected. Paratuberculosis in cattle was almost unknown in the Czech Republic until 1990. An increase in the number of cows with paratuberculosis found in slaughterhouses and the incidence of Crohn's disease in the last decade is evident. The possible risk of MAP dead cells or bacterial structures in food is discussed in respect to autoimmune Crohn's disease. The national programmes of paratuberculosis control and certification of paratuberculosis-free herds should be strongly supported to decrease the risk for children and other people under higher risk. Producers should use MAP free milk for baby food production on a voluntary basis.

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“…XX w. ukazało się także wiele prac potwierdzających obecność Map w produktach mlecznych. Obecność żywych komórek tych drobnoustrojów stwierdzono w próbkach rynkowego mleka pasteryzowanego w Czechach, Wielkiej Brytanii, Stanach Zjednoczonych i Indiach [2,6,16,28], a także w próbkach serów w Czechach i Grecji [19].…”

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Thermal Resistance of Mycobacterium Avium Subsp. Paratuberculosis

Berthold-Pluta

Pluta

Olkowski

et al. 2015

zntj

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CIEPŁOOPORNOŚĆ MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSISS t r e s z c z e n i e W latach 90. XX w. ukazały się pierwsze publikacje wskazujące, że Mycobacterium avium subsp. paratuberculosis (Map) są bardziej ciepłooporne niż M. bovis i przeżywają pasteryzację mleka HTST. Od tego czasu ukazało się wiele prac potwierdzających obecność Map w produktach mlecznych. Obecność żywych komórek tych drobnoustrojów stwierdzono w próbkach rynkowego mleka pasteryzowanego, a także w serach. Badania nad ciepłoopornością Map są niełatwe ze względu na uciążliwość hodowli, a porównanie i interpretacja danych literaturowych z tego zakresu są utrudnione z uwagi na różnice w stosowanych metodach eksperymentalnych. W badaniach przeprowadzanych z użyciem mleka ogrzewanego w probówkach, kapilarach lub w pasteryzatorach laboratoryjnych wykazano, że pasteryzacja w temp. 63 ºC/30 min, jak i HTST zmniejszają liczbę Map o 4 -7 rzędów logarytmicznych. Rozbieżne wyniki otrzymywano w doświadczeniach prowadzonych w warunkach pasteryzacji przemysłowej. Istnieje kilka hipotez oporności cieplnej Map w mleku, m.in. tworzenie skupisk komórek, adaptacja fizjologiczna prowadząca do nabycia ciepłooporności oraz fizykochemiczne zmiany we wnętrzu komórki.Słowa kluczowe: Mycobacterium avium subsp. paratuberculosis, obróbka cieplna, pasteryzacja, HTST Wprowadzenie Mycobacterium avium subsp. paratuberculosis (Map) jako pierwsi wyizolowali Johne i Frottingham w 1895 r. w badaniach przyczyn przewlekłej biegunki u bydła, nazwanej chorobą Johnego. Uważa się za prawdopodobne, że drobnoustroje te odpowiadają także za przewlekły stan zapalny przewodu pokarmowego człowieka (chorobę Crohna). Cechą charakterystyczną Map jest szczególna budowa 3-warstwowej ściany komórkowej złożonej w 30 ÷ 40 % z lipidów, dzięki której drobnoustroje te są oporne dr hab. A. Pluta, prof. SGGW, mgr inż. M. Olkowski, inż. A. Ostrowska, Katedra Biotechnologii, Mikrobiologii i Oceny Żywności, Wydz. Nauk o Żywności, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie, ul. Nowoursynowska 159C,

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Detection ofMycobacterium aviumsubsp.paratuberculosisin Retail Cheeses from Greece and the Czech Republic

Cited by 82 publications

References 19 publications

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Evalution of “indirect fluorescent antibody test‟ as potential screening test for Mycobacterium avium subspecies paratuberculosis using milk of lactating domestic livestock

Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved

Thermal Resistance of Mycobacterium Avium Subsp. Paratuberculosis

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