DETECTION OF <i>SALMONELLA TYPHIMURIUM</i> IN OYSTERS BY PCR AND MOLECULAR HYBRIDIZATION

Corrêa, Adriana Abreu; Toso, Javier; Albarnaz, Jonas D.; Simões, C. M. O.; Barardi, Célia Regina Monte

doi:10.1111/j.1745-4557.2006.00086.x

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…Our results are in agreement with Corrêa et al . () which showed that extraction of DNA from enrichment broth on food samples is more efficient than DNA extraction directly from the sample, because the enrichment broth may dilute the presence of PCR inhibitor substances. However, Nascimento and Sand (), when searching for the ETEC toxins lt and st, directly from the enrichment broths did not identify any genes.…”

Section: Resultsmentioning

confidence: 99%

Survey of Pathogens Isolated from Mussels Perna Perna Collected in Rocky Shore and Fishmarket of Niterói, RJ, and Their Respective Resistance Profile to Antimicrobial Drugs

Jayme

Silva

Sales

et al. 2016

Journal of Food Quality

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To assess bacteriological safety of mussels Perna perna, harvested in Itaipu, Niter oi, RJ and purchased at São Pedro fishmarket at the same city, fecal coliform counts and detection of Escherichia coli, Salmonella spp. and Vibrio spp. was performed in 27 mussel and 9 seawater samples collected between March 2012 and April of 2013. The pathogens isolated were submitted to antimicrobial susceptibility test.The majority (74%) of the mussel samples were unfit for human consumption and 89% of the seawater samples had unsatisfactorily high levels of contamination according to Brazilian laws. From the 77 E. coli and 51 Vibrio spp. isolated, 13% and 68.6% showed multiresistance and 15.6% and 72.5% showed multidrug resistant, respectively. The only Salmonella strain isolated was susceptible to all antimicrobial tested. Serotypes of Salmonella spp. and V. cholerae were not detected from the enrichment broths. Water pollution and food manipulation, throughout the food production chain, were factors which influenced the contamination of mussels. PRACTICAL APPLICATIONSThis is the first report studying microbial quality of mussels along their process before arriving at the market; including characteristics of virulence genes and antimicrobial resistance of pathogens. Our study demonstrated that after collection, the manipulation realized by mussel's pickers is an important source of contamination, warning to the necessity of a better monitoring in the commercialization of this seafood.

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Section: Resultsmentioning

confidence: 99%

Survey of Pathogens Isolated from Mussels Perna Perna Collected in Rocky Shore and Fishmarket of Niterói, RJ, and Their Respective Resistance Profile to Antimicrobial Drugs

Jayme

Silva

Sales

et al. 2016

Journal of Food Quality

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“…The global concern of food-borne illness, particularly from ingestion of food-borne bacteria, [1][2][3] necessitates rapid detection within food and environmental matrices. Typically, bacterial cultures, 4,5 DNA-based methods, [6][7][8][9][10] and antibody-based detection schemes 11,12 are used to isolate or identify bacteria. Cultural methodology is time-consuming; bacterial pathogens often occur in low numbers and an enrichment procedure, ranging from hours to days, may be required prior to the application of a rapid detection assay.…”

Section: Introductionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) has been reported as one of the most promising methods for detection of pathogens, particularly Salmonella. 6,7 However, PCR-based assays must include a means to circumvent the frequent presence of inhibitors, may detect the presence of DNA, which may not be indicative of viable bacteria, and lacks sensitivity unless bacterial numbers are sufficiently high.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Spectroscopy for Rapid Detection and Classification of Bacterial Pathogens

et al. 2009

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This study deals with the rapid detection and differentiation of Escherichia coli, Salmonella, and Campylobacter, which are the most commonly identified commensal and pathogenic bacteria in foods, using fluorescence spectroscopy and multivariate analysis. Each bacterial sample cultured under controlled conditions was diluted in physiologic saline for analysis. Fluorescence spectra were collected over a range of 200-700 nm with 0.5 nm intervals on the PerkinElmer Fluorescence Spectrometer. The synchronous scan technique was employed to find the optimum excitation (lambda(ex)) and emission (lambda(em)) wavelengths for individual bacteria with the wavelength interval (Deltalambda) being varied from 10 to 200 nm. The synchronous spectra and two-dimensional plots showed two maximum lambda(ex) values at 225 nm and 280 nm and one maximum lambda(em) at 335-345 nm (lambda(em) = lambda(ex) + Deltalambda), which correspond to the lambda(ex) = 225 nm, Deltalambda = 110-120 nm, and lambda(ex) = 280 nm, Deltalambda = 60-65 nm. For all three bacterial genera, the same synchronous scan results were obtained. The emission spectra from the three bacteria groups were very similar, creating difficulty in classification. However, the application of principal component analysis (PCA) to the fluorescence spectra resulted in successful classification of the bacteria by their genus as well as determining their concentration. The detection limit was approximately 10(3)-10(4) cells/mL for each bacterial sample. These results demonstrated that fluorescence spectroscopy, when coupled with PCA processing, has the potential to detect and to classify bacterial pathogens in liquids. The methodology is rapid (>10 min), inexpensive, and requires minimal sample preparation compared to standard analytical methods for bacterial detection.

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“…The majority of worldwide outbreaks associated with bivalve shellfish have been linked to oysters, and then to clams and mussels. Oysters, which are filter feeders, often concentrate pathogenic bacteria from marine and brackish waters (8). Rising ocean temperatures seem to contribute to observed increases in outbreaks associated with oysters (27).…”

mentioning

confidence: 99%

Carvacrol and Cinnamaldehyde Inactivate Antibiotic-Resistant Salmonella enterica in Buffer and on Celery and Oysters

Ravishankar

Zhu

Reyna-Granados

et al. 2010

Journal of Food Protection

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The emergence of antibiotic-resistant Salmonella is of concern to food processors. The objective of this research was to identify antimicrobial activities of cinnamaldehyde and carvacrol against antibiotic-resistant Salmonella enterica in phosphate-buffered saline (PBS) and on celery and oysters. Twenty-three isolates were screened for resistance to seven antibiotics. Two resistant and two susceptible strains were chosen for the study. S. enterica cultures (10(5) CFU/ml) were added to different concentrations of cinnamaldehyde and carvacrol (0.1, 0.2, 0.3, and 0.4% [vol/vol]) in PBS, mixed, and incubated at 37 degrees C. Samples were taken at 0, 1, 5, and 24 h for enumeration. Celery and oysters were inoculated with S. enterica (10(6-7) CFU/ml), treated with 1% cinnamaldehyde or 1% carvacrol, incubated at 4 degrees C, and then sampled for enumeration on days 0 and 3. Both antimicrobials induced complete inactivation of S. enterica in PBS at 0.3 and 0.4% on exposure, and on 0.2% in 1 h. Exposure to cinnamaldehyde at 0.1% inactivated all pathogens at 1 h, and survivors were observed only for Salmonella Newport with 0.1% carvacrol at 1 h. In celery, 1% carvacrol reduced S. enterica populations to below detection on day 0, while 1% cinnamaldehyde reduced populations by 1 and 2.3 log on day 0 and day 3, respectively. In oysters, both antimicrobials caused about 5-log reductions on day 3. These results show the potential antimicrobial effects of carvacrol and cinnamaldehyde against antibiotic-resistant S. enterica in vitro and in foods.

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DETECTION OF SALMONELLA TYPHIMURIUM IN OYSTERS BY PCR AND MOLECULAR HYBRIDIZATION

Cited by 12 publications

References 25 publications

Survey of Pathogens Isolated from Mussels Perna Perna Collected in Rocky Shore and Fishmarket of Niterói, RJ, and Their Respective Resistance Profile to Antimicrobial Drugs

Survey of Pathogens Isolated from Mussels Perna Perna Collected in Rocky Shore and Fishmarket of Niterói, RJ, and Their Respective Resistance Profile to Antimicrobial Drugs

Fluorescence Spectroscopy for Rapid Detection and Classification of Bacterial Pathogens

Carvacrol and Cinnamaldehyde Inactivate Antibiotic-Resistant Salmonella enterica in Buffer and on Celery and Oysters

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