Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor

Farid, Sidra; Meshik, Xenia; Choi, Min Sun; Mukherjee, Souvik; Lan, Yi; Parikh, D.; Poduri, Shripriya; Baterdene, Undarmaa; Huang, Ching En; Wang, Yung Yu; Burke, Peter J.; Dutta, Mitra; Stroscio, Michael A.

doi:10.1016/j.bios.2015.04.047

Cited by 131 publications

(77 citation statements)

References 24 publications

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“…The ACP only and ACP/HDT-MCH functionalized Au IDEs were characterized for sensitivity to background interfering proteins including FBS and BSA (Figure 4). Figure 4a shows the electrochemical response of the aptasensors with increasing incubation times (5,15,30, and 60 min) of pure FBS (see Methods section). These selectivity experiments show that the change of R ct is negligible for the Au IDEs modified with the ternary monolayer ACP/ HDT-MCH, while the Au IDE modified with ACP only exhibited increasing R ct in the presence of the target.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…11,12 Besides these conventional laboratory methods, other more rapid sensing methods for IFN-γ have been developed including field effect transistor based biosensors (Bio-FETs) and Forster resonance energy transfer (FRET) aptamer beacons. 4,14,15 These reported biosensing techniques display tremendous promise toward sensitive and rapid IFN-γ sensing with detection limits extending down into the nanomolar and even picomolar regimes. However, BioFETs that comprise micro/nano components require extensive cleanroom manufacturing steps (e.g., deposition of active source/drain regions, nano/microwire gate development, gate oxide growth, metal contact and reference electrode deposition, and passivation layer formation) that substantially increase the cost of the biosensor and decrease its reproducibility (e.g., any crack/break in the passivation layer would interfere with the sensor signal during biological sensing).…”

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confidence: 99%

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Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array

Ding¹,

Mosher²,

Lee³

et al. 2017

ACS Sens.

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A label-free electrochemical impedance spectroscopy (EIS) aptasensor for rapid detection (<35>min) of interferon-gamma (IFN-γ) was fabricated by immobilizing a RNA aptamer capture probe (ACP), selective to IFN-γ, on a gold interdigitated electrode array (Au IDE). The ACP was modified with a thiol group at the 5′ terminal end and subsequently co-immobilized with 1,6-hexanedithiol (HDT) and 6-mercapto-1-hexanolphosphate (MCH) to the gold surface through thiol-gold interactions. This ACP/ HDT-MCH ternary surface monolayer facilitates efficient hybridization with IFN-γ and displays high resistance to nonspecific adsorption of nontarget proteins [i.e., fetal bovine serum (FBS) and bovine serum albumin (BSA)]. The Au IDE functionalized with ACP/HDT-MCH was able to measure IFN-γ in actual FBS solution with a linear sensing range from 22.22 pM to 0.11 nM (1-5 ng/mL) and a detection limit of 11.56 pM. The ability to rapidly sense IFN-γ within this sensing range makes the developed electrochemical platform conducive toward in-field disease detection of a variety of diseases including paratuberculosis (i.e., Johne's Disease). Furthermore, experimental results were numerically validated with an equivalent circuit model that elucidated the effects of the sensing process and the influence of the immobilized ternary monolayer on signal output. This is the first time that ternary surface monolayers have been used to selectively capture/detect IFN-γ on Au IDEs. ABSTRACT: A label-free electrochemical impedance spectroscopy (EIS) aptasensor for rapid detection (<35 min) of interferon-gamma (IFN-γ) was fabricated by immobilizing a RNA aptamer capture probe (ACP), selective to IFN-γ, on a gold interdigitated electrode array (Au IDE). The ACP was modified with a thiol group at the 5′ terminal end and subsequently coimmobilized with 1,6-hexanedithiol (HDT) and 6-mercapto-1-hexanolphosphate (MCH) to the gold surface through thiol−gold interactions. This ACP/HDT-MCH ternary surface monolayer facilitates efficient hybridization with IFN-γ and displays high resistance to nonspecific adsorption of nontarget proteins [i.e., fetal bovine serum (FBS) and bovine serum albumin (BSA)]. The Au IDE functionalized with ACP/HDT-MCH was able to measure IFN-γ in actual FBS solution with a linear sensing range from 22.22 pM to 0.11 nM (1−5 ng/mL) and a detection limit of 11.56 pM. The ability to rapidly sense IFN-γ within this sensing range makes the developed electrochemical platform conducive toward in-field disease detection of a variety of diseases including paratuberculosis (i.e., Johne's Disease). Furthermore, experimental results were numerically validated with an equivalent circuit model that elucidated the effects of the sensing process and the influence of the immobilized ternary monolayer on signal output. This is the first time that ternary surface monolayers have been used to selectively capture/detect IFN-γ on Au IDEs.

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Section: ■ Results and Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array

Ding¹,

Mosher²,

Lee³

et al. 2017

ACS Sens.

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“…Recently, various FET-based immunosensors using aptamers immobilized on nanomaterial sensing channels were reported [135][136][137][138][139][140][141][142][143]. For instance, So et al firstly demonstrated a FET biosensor based on SWCNT using aptamers as bioreceptors.…”

Section: Aptamers Instead Of Antibodiesmentioning

confidence: 99%

“…They were detected with LOD of 1.04 nM and 104 pM for n-type and p-type SiNW-FET, respectively. Interferon-gamma (IFN-γ) can be used as a biomarker to diagnose infectious diseases like tuberculosis [135]. This cytokine was sensitively detected through a graphene-FET immunosensor using IFN-γ DNA aptamers immobilized on the graphene surface.…”

Section: Aptamers Instead Of Antibodiesmentioning

confidence: 99%

Recent Trends in Field-Effect Transistors-Based Immunosensors

Moraes

Kubota

2016

Chemosensors

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Abstract:Immunosensors are analytical platforms that detect specific antigen-antibody interactions and play an important role in a wide range of applications in biomedical clinical diagnosis, food safety, and monitoring contaminants in the environment. Field-effect transistors (FET) immunosensors have been developed as promising alternatives to conventional immunoassays, which require complicated processes and long-time data acquisition. The electrical signal of FET-based immunosensors is generated as a result of the antigen-antibody conjugation. FET biosensors present real-time and rapid response, require small sample volume, and exhibit higher sensitivity and selectivity. This review brings an overview on the recent literature of FET-based immunosensors, highlighting a diversity of nanomaterials modified with specific receptors as immunosensing platforms for the ultrasensitive detection of various biomolecules.

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“…Nanostructures exhibiting LSPR are used for many potential applications such as in medical, sensing, lithography and optoelectronic devices [1][2][3][4]. The LSPR is observed due to the light induced collective oscillation of free electrons, resulting in strong absorption, scattering and local field enhancement and this is responsible for surface-enhanced Raman scattering (SERS) and other surface-enhanced spectroscopic processes.…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of electric field enhancement in ZnO film with plasmonic au quantum dots

Poduri

Choi

Dutta

et al. 2015

2015 International Workshop on Computational Electronics (IWCE)

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ZnO nanostructures have generated interest for a wide range of applications due to their unique optical and electrical properties. Especially, the spontaneous polarization in the ZnO nanostructure which produces a permanent strong static electric field that can be applied to many studies. Also, their strong polarizability makes them potential candidates for many applications such as ion channel modulation, photodetector and LEDs in UV range. In this paper, the plasmonic enhancement of the induced electric field produced from ZnO nanostructures is computed by numerical analysis.

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Detection of Interferon gamma using graphene and aptamer based FET-like electrochemical biosensor

Cited by 131 publications

References 24 publications

Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array

Rapid and Label-Free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprising a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array

Recent Trends in Field-Effect Transistors-Based Immunosensors

Numerical analysis of electric field enhancement in ZnO film with plasmonic au quantum dots

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