Detection of Marteilia refringens using nested PCR and in situ hybridisation in Chamelea gallina from the Balearic Islands (Spain)

López-Flores, Inmaculada; Robles, Francisca; Valencia, José María; Grau, Amàlia; Villalba, António; Herrán, Roberto de la; Garrido-Ramos, Manuel A.; Ruíz-Rejón, Carmelo; Ruíz-Rejón, Manuel; Navas, J.I.

doi:10.3354/dao01966

Cited by 26 publications

(13 citation statements)

References 34 publications

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“…Two distinct sources of DNA were employed: (a) two paraffin blocks in whose histological sections infection by Marteilia sp. was diagnosed (accordingly with the methodology by López-Flores et al (2008); and (b)) tissues of pygmy mussels from sample B. The universal primers used are indicated in Table 1.…”

Section: Pathological Conditionmentioning

confidence: 99%

The mussel Xenostrobus securis: a well-established alien invader in the Ria de Vigo (Spain, NE Atlantic)

et al. 2009

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Biological and habitat characterization of the non-indigenous invasive species Xenostrobus securis was undertaken in the Ria de Vigo. This study included genetic identification of mussel samples collected from introduced and endemic areas, and the assessment of mussel size, population abundance, geographic distribution, pathological condition, and sediment composition of substrata type. The mussel had a marked patchy distribution, being more abundant in brackish sites with fine sediments and high organic matter content. Pathological analysis revealed that X. securis does not play any role as vector for introducing allochthonous pathogens in the Ria de Vigo. Nevertheless, depending on its invasiveness potential, the mussel could be a key host favouring spreading and epizootic outbreaks of marteliosis which is known to be harmful for local bivalve populations. Phylogenetic analyses of the COI gene placed all the resulting sequences in a clade within the genus Xenostrobus and its phylogeny congruent with an Australian/Pacific origin. The COI tree suggests two historical introductions in European waters. One of these invasions seems to have started in Galicia, moving from there towards Italy and France, while the geographical spread of the second invasion cannot be deciphered, although the Australian/Pacific origin of this invasion seems very possible. The 18S network is congruent with one invasion starting in Galicia or in Italy, as the Australian haplotype is closely related to the haplotype found in these areas. Several hypotheses accounting for the colonization history of this species in Galician waters are discussed.

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Section: Pathological Conditionmentioning

confidence: 99%

The mussel Xenostrobus securis: a well-established alien invader in the Ria de Vigo (Spain, NE Atlantic)

et al. 2009

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“…Subsequent studies have reinforced these findings, although the degree of affinity of M-type to mussels and O-type to oysters has not been analysed. Furthermore, the host range of M. refringens is clearly wider than O. edulis and M. edulis : both types being detected in Mytilus galloprovincialis (Novoa et al 2005 ; Balseiro et al 2007 ) and the M-type in clam Solen marginatus (López-Flores et al 2008 b ) and the mussel Xenostrobus securis (Pascual et al 2010 ) and, the O-type in the clam Chamelea gallina (López-Flores et al 2008 a ). Berthe et al ( 2004 ) point out that unidentified Marteilia isolates have also been observed in many bivalve species naturally present in the geographic range of M. refringens .…”

Section: Introductionmentioning

confidence: 99%

Marteilia refringens and Marteilia pararefringens sp. nov. are distinct parasites of bivalves and have different European distributions

et al. 2018

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Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini, which were synonymized in 2007 and subsequently referred to as M. refringens ‘O-type’ and ‘M-type’. O-type has caused mass mortalities of the flat oyster Ostrea edulis. We used high throughput sequencing and histology to intensively screen flat oysters and mussels (Mytilus edulis) from the UK, Sweden and Norway for infection by both types and to generate multi-gene datasets to clarify their genetic distinctiveness. Mussels from the UK, Norway and Sweden were more frequently polymerase chain reaction (PCR)-positive for M-type (75/849) than oysters (11/542). We did not detect O-type in any northern European samples, and no histology-confirmed Marteilia-infected oysters were found in the UK, Norway and Sweden, even where co-habiting mussels were infected by the M-type. The two genetic lineages within ‘M. refringens’ are robustly distinguishable at species level. We therefore formally define them as separate species: M. refringens (previously O-type) and Marteilia pararefringens sp. nov. (M-type). We designed and tested new Marteilia-specific PCR primers amplifying from the 3’ end of the 18S rRNA gene through to the 5.8S gene, which specifically amplified the target region from both tissue and environmental samples.

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“…and Marteilioides spp. are the main focus of research (López-Flores et al 2008b). Based on molecular evidence, Marteilia spp.…”

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confidence: 99%

A Marteilia‐Like Parasite in Blue Mussels Mytilus edulis in China

Wang

Lu²,

Liang

et al. 2012

J Aqua Anim Hlth

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Species of the genus Marteilia (Phylum Paramyxea) are protozoan parasites of marine mollusks. Marteilia spp. have been detected in mollusks from different parts of the world, but the presence of these parasites in China has not been previously reported. Therefore, a survey was conducted to look for the presence of Marteilia spp. in blue mussels Mytilus edulis and Asian green mussels Perna viridis collected along China's coasts. Histological and PCR analyses revealed that 5 of 180 M. edulis (prevalence = 2.8%) were positive for infection with a Marteilia-like organism, whereas the parasite was not detected in any of the 80 P. viridis individuals tested. Total genomic DNA was extracted from the infected tissue sections for PCR amplification. The PCR amplification with Marteilia primers SS1 and SAS1 yielded the expected 641-bp product. Sequencing results showed that the 18S ribosomal RNA gene fragment from the protozoans found in M. edulis from China was 88% similar to that of Marteilia refringens, a species that was reported from M. edulis and European flat oysters Ostrea edulis collected in France. This is the first report of a Marteilia-like organism infecting M. edulis in China.

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Detection of Marteilia refringens using nested PCR and in situ hybridisation in Chamelea gallina from the Balearic Islands (Spain)

Cited by 26 publications

References 34 publications

The mussel Xenostrobus securis: a well-established alien invader in the Ria de Vigo (Spain, NE Atlantic)

The mussel Xenostrobus securis: a well-established alien invader in the Ria de Vigo (Spain, NE Atlantic)

Marteilia refringens and Marteilia pararefringens sp. nov. are distinct parasites of bivalves and have different European distributions

A Marteilia‐Like Parasite in Blue Mussels Mytilus edulis in China

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