Detection of measles virus antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis

Wrzos, Helena; Kulczycki, J; Laskowski, Zdzisław; Matacz, D.; Brzosko, W. J.

doi:10.1007/bf01317500

Cited by 23 publications

(10 citation statements)

References 6 publications

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“…Although attempts to reactivate the virus by immunosuppression (5 months after infection) failed, a number of non-immunosuppressed animals became paralysed 13 to 17 months after infection and CDV antigens could be detected in the brain and mesenteric lymph nodes. This situation may be analogous to measles virus-induced SSPE where, as well as the neurological lesions, virus has been found in lymphocytes (Wrzos et al, 1979) and lymph nodes (Horta-Barbosa et al, 1971). …”

Section: Discussionmentioning

confidence: 99%

Canine Distemper Infection in Mice: Characterization of a Neuro-adapted Virus Strain and its Long-term Evolution in the Mouse

Bernard¹,

Wild²,

Tripier

1983

Journal of General Virology

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SUMMARYThe Onderstepoort strain of canine distemper virus (CDV) which has been adapted to newborn Swiss mice was used in a study in weanling mice. Intracerebral inoculation of newborn mice with either the parent or mouse-adapted virus strain led to mortality in 100~ of the animals. In weanling mice the parent virus produced less than 10~o mortality, whereas the mouse-adapted strain killed approximately 40 ~ of the animals and this was not dose-dependent. Although a meningitis was observed in the surviving mice, the occurrence of viral antigens was less widespread in the brain of weanling mice than in the brain of newborn mice, and could not be detected more than 5 months after infection. In long-term experiments two phenomena were observed. At 4 to 6 months post-infection up to 30 ~ of the mice became obese; however, no viral antigens were detected in the brains. At 13 to 17 months post-infection a number of mice became paralysed and virus antigen could be detected in the brain and lymph glands; however, infectious virus could not be isolated. These observations are discussed in relation to neurological infection and metabolic disease.

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Section: Discussionmentioning

confidence: 99%

Canine Distemper Infection in Mice: Characterization of a Neuro-adapted Virus Strain and its Long-term Evolution in the Mouse

Bernard¹,

Wild²,

Tripier

1983

Journal of General Virology

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“…ln subacute sclerosing panencephalitis (SSPE) either MV antigens or RNA have been detected by some groups in PBMCs of patients with this disease in the absence of any major alteration of the immune response (Horta-Barbosa, 1 979; Wrosz et al, 1979;Fournier et al, 1985Fournier et al, , 1988. ln chronic active hepatitis, in systemic Iupus erythematosis andin glomerulonephritis as weil as in healthy controls unconfirmed reports describe the presence of MV specific RNA by dot blot or in situ hybridization in PBMCs of these patients suggesting that MV may persist in lymphoid cells after acute measles and is not cleared by the host defence mechanism (Robertson et al, 1987;Andjaparidze et al, 1989).…”

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confidence: 99%

Expression of measles virus RNA in peripheral blood mononuclear cells of patients with measles, SSPE, and autoimmune diseases

et al. 1991

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ln order to characterize measles virus (MV} infection in peripheral blood mononuclear cells (PBMCs), RNA was isolated from PBMCs after PHA-stimulation for 72 hr of 9 patients with acute measles, 16 patients with subacute sclerosing panencephalitis (SSPE), 13 patients with various autoimmune diseases, and 16 healthy control donors. The RNA obtained was screened for the presence of MV N (nucleocapsid) gene specific transcripts of either positive or negative orientation in a S 1 nuclease protection assay. The sensitivity of this assay allowed us to detect one infected cell in 20,000 PBMCs or 0.1 to 0.05 copies of MV-specific RNA per cell. Using single-stranded DNA or RNA probes expression of MV genomic RNA of negative polarity could be detected in only one case of acute measles and one healthy control donor. Conversely, N-specific transcripts of positive polarity, indicating active transcription, could only be detected in patients with acute measles. ln addition, in infected PBMCs andin a persistently MV-infected B cellline positive stranded N-specific transcripts containing Ieader usually present at very low frequency have been found in relatively increased amounts in comparison with transcripts lacking Ieader. Whereas the ratio of these RNA species du ring lytic infection with MV in Vero cells is about 1:50, the ratio found here ranges from 1:3 to 1:10. This altered ratio indicates a specific regulation of MV specific transcription in cells of lymphoid origin that has not been found in any Other cell system analyzed.

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“…In each case, the virus appears to persist in lymphoid cells of peripheral blood despite the presence of rubella-specific antibody in serum, The present study was undertaken to investigate the possible role of antibody in the maintenance of this persistent infection. An analogous situation is found in subacute sclerosing panencephalitis where measles virus has been shown to persist in both brain and lymphoid tissue of patients with high levels of measles antibody (Payne et al, 1969;Horta-Barbosa et al, 1971 ;Wrzos et al, 1979). In measles virus infections in vitro, antibody has been shown to exert two effects, causing modulation or 'capping' of viral antigens at the cell surface (Oldstone & Tishan, 1978;Gorman & Lachmann, 1982), and also a differential inhibition of the synthesis of certain measles virus proteins as measured by [35Slmethionine incorporation (Fujinami & Oldstone, 1979, I980).…”

Section: Introductionmentioning

confidence: 73%

The Effect of Antibody on Rubella Virus Infection in Human Lymphoid Cells

Chantler

Davies

1987

Journal of General Virology

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SUMMARYThe effect of rabbit anti-rubella virus (RV) serum on the course of RV infection of human lymphoid cells has been examined. The antibody was found to abolish viral replication such that no virus progeny could be detected either extracellularly (after removal of the antiserum) or intracellularly, in the treated cells. Viral protein synthesis was also found to be totally inhibited in the presence of anti-RV but resumed immediately on removal of the antibody block at levels suggesting that viral RNA had been accumulating in the cell. Infectious focus assays indicated that the effect could be explained in part by the restriction by antiviral antibody of cell-to-cell spread in the indicator monolayer. Thus by 48 h when 1009/oo of lymphoid cells infected with virus alone were capable of forming infectious foci, only 40 to 50~ of cells infected in the presence of antiviral antibody produced loci. However this restriction imposed by antibody did not adequately explain the total inhibition of viral protein synthesis that occurred under these conditions. Pretreatment of RV with excess neutralizing antiserum reduced the virus titre > 99.9 ~ but did not eliminate infectious virus. The

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Detection of measles virus antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis

Cited by 23 publications

References 6 publications

Canine Distemper Infection in Mice: Characterization of a Neuro-adapted Virus Strain and its Long-term Evolution in the Mouse

Canine Distemper Infection in Mice: Characterization of a Neuro-adapted Virus Strain and its Long-term Evolution in the Mouse

Expression of measles virus RNA in peripheral blood mononuclear cells of patients with measles, SSPE, and autoimmune diseases

The Effect of Antibody on Rubella Virus Infection in Human Lymphoid Cells

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