Detection of melatonin and homocysteine simultaneously in ulcerative colitis

Chen, Moli; Qiao, Mu; Xu, Jianming; Lu, Chong‐Dao; Hou, Fang; Liu, Xiaochang

doi:10.1016/j.cca.2011.06.036

Cited by 22 publications

(13 citation statements)

References 28 publications

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“…The levels of plasma melatonin in patients with ulcerative colitis (UC) have been reported to be significantly lower than those of healthy control[36]. They are higher in remission state than in active disease.…”

Section: Discussionmentioning

confidence: 99%

Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Kim¹,

Park²,

Baik³

et al. 2016

WJG

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AIMTo determine adiponectin expression in colonic tissue of murine colitis and systemic cytokine expression after melatonin treatments and sleep deprivation.METHODSThe following five groups of C57BL/6 mice were used in this study: (1) group I, control; (2) group II, 2% DSS induced colitis for 7 d; (3) group III, 2% DSS induced colitis and melatonin treatment; (4) group IV, 2% DSS induced colitis with sleep deprivation (SD) using specially designed and modified multiple platform water baths; and (5) group V, 2% DSS induced colitis with SD and melatonin treatment. Melatonin (10 mg/kg) or saline was intraperitoneally injected daily to mice for 4 d. The body weight was monitored daily. The degree of colitis was evaluated histologically after sacrificing the mice. Immunohistochemical staining and Western blot analysis was performed using anti-adiponectin antibody. After sampling by intracardiac punctures, levels of serum cytokines were measured by ELISA.RESULTSSleep deprivation in water bath exacerbated DSS induced colitis and worsened weight loss. Melatonin injection not only alleviated the severity of mucosal injury, but also helped survival during stressful condition. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. The expression levels of IL-6 and IL-17 were increased in the serum of mice with DSS colitis but decreased after melatonin injection.CONCLUSIONThis study suggested that melatonin modulated adiponectin expression in colonic tissue and melatonin and adiponectin synergistically potentiated anti-inflammatory effects on colitis with sleep deprivation.

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Section: Discussionmentioning

confidence: 99%

Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Kim¹,

Park²,

Baik³

et al. 2016

WJG

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show abstract

“…Melatonin is a material that protects the intestines from cytokine and neurotransmitters associated with stress, and the plasma level of melatonin is significantly lower in patients with UC. 36 It was shown to be effective in some clinical trials and animal models of IBD in ameliorating the condition. 37,38 Bright light inhibits melatonin secretion.…”

Section: Discussionmentioning

confidence: 99%

Phototherapy with artificial light suppresses dextran sulfate sodium‐induced colitis in a mouse model

Ito

Goto

Oyama

et al. 2014

J of Gastro and Hepatol

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“…It was also found that in the acute phase of inflammation the increase in the number of EC cells does not result in increased urinary aMT6s excretion. Moreover, other researchers observed a reduction of melatonin level in patients with ulcerative colitis (21).…”

Section: Introductionmentioning

confidence: 89%

Evaluation of the Extrapineal Sources of Melatonin in Patients with Lymphocytic Colitis

Chojnacki¹,

Błasiak²,

Fichna³

et al. 2017

IJMCR

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Melatonin can be synthesized by different cells in the gastrointestinal tract. The aim of this study was to evaluate the expression of melatonin-synthesizing enzymes: arylalkylamine-N-acetyltransferase(AANAT) and N-acetylserotonin methyltransferase (ASMT) in colonic mucosa and urinary 6-sulfatoxymelatonin excretion(aMT6s) in patients with lymphocytic colitis(LC) related to the number of intraepithelial lymphocytes(IEL) and enterochromaffin cells (EC). The study included 32 healthy subjects (HS group) and 36 patients with lymphocytic colitis (LC group). The diagnosis was based on histological and immunohistochemical performances using mouse monoclonal antibodies (chromogranin A –LK 2H10). EC were counted in 10 fields of each bioptate at 40x magnification. The level of mRNA expression of AANAT and ASMT was estimated in colonic mucosa with RT-PCR. The urine concentration of aMT6s was determined by photometric method. Significant differences were found in obtained results between HS and LC group: the number of IEL 14.1±3.41 vs. 32.4±5.50 per 100 surface colonocytes (p less than 0.001); the number of EC 32.2±10.1 vs. 70.4±21.2 (p less than 0.001); AANAT -1.32±0.47 vs. 2.32±0.65 (pless than 0.001; ASMT – 1.51±0.59 vs. 2.80±0.92 (p less than 0.001) aMT6s- 13.4±4.87 vs. 19.9±6.29 (p less than 0.001). Moreover, a positive correlation was found between the number of EC and ASMT (p= 0.011) and between EC and aMT6s (p less than 0.001). No correlation occurred between the number of IEL and AANAT p=0.932) and AMST (n=0.536) and aMT6s (p=9.288). These results indicate that the enterochromaffin cells in colonic mucosa are the main extrapineal source of melatonin in patients with lymphocytic colitis.

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Detection of melatonin and homocysteine simultaneously in ulcerative colitis

Cited by 22 publications

References 28 publications

Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Melatonin modulates adiponectin expression on murine colitis with sleep deprivation

Phototherapy with artificial light suppresses dextran sulfate sodium‐induced colitis in a mouse model

Evaluation of the Extrapineal Sources of Melatonin in Patients with Lymphocytic Colitis

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