Detection of Porcine circovirus 3 from captured wild boars in Korea

Dhandapani, Gowtham; Yoon, Sun‐Woo; Noh, Ji Yeong; Jang, Seong Sik; Han, Sang-Hoon; Jeong, Dae Gwin; Kim, Hye Kwon

doi:10.1002/vms3.518

Cited by 8 publications

(9 citation statements)

References 31 publications

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“…In this study, positive rates of PCV2 between 2018 and 2019 were much higher (18% and 46.3% in lung and HLN, respectively). Other Korean research teams have demonstrated that positive rates of PCV3 in whole blood, serum, and nasal swabs (Dhandapani et al, 2021) and whole blood (Song et al, 2021) samples of wild boars were 5.6% and 0.01 %, respectively. Although we cannot rule out the possibility of other influences, these rapidly increasing positive rates of PCV3 in this study might be due to sample differences (Kixmöller et al, 2008;J.…”

Section: ) the Discrepancy Of The Target Cells Between Pcv2 And Pcv3mentioning

confidence: 99%

“…Wild boar (Sus scrofa) is a susceptible host and suspected to be an important reservoir of pathogens infecting commercial pigs and humans (Dhandapani et al, 2021;Prinz et al, 2019). PCV2 and PCV3…”

Section: Introductionmentioning

confidence: 99%

“…have also been detected in wild boars in many countries including Germany, China, Brazil, Spain, Italy, and South Korea (Dhandapani et al, 2021;Sirisereewan et al, 2022;Song et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Wild boar ( Sus scrofa ) is a susceptible host and suspected to be an important reservoir of pathogens infecting commercial pigs and humans (Dhandapani et al., 2021 ; Prinz et al., 2019 ). PCV2 and PCV3 have also been detected in wild boars in many countries including Germany, China, Brazil, Spain, Italy, and South Korea (Dhandapani et al., 2021 ; Sirisereewan et al., 2022 ; Song et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of porcine circovirus type 2 and type 3 in slaughtered pigs and wild boars in Korea

Park,

Kim,

Jeong

et al. 2023

Veterinary Medicine & Sci

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BackgroundPorcine circovirus, a non‐enveloped single‐stranded DNA virus belonging to the genus Circovirus of the family Circoviridae, is a major pathogen of porcine circovirus‐associated disease. Porcine circovirus 3, a novel porcine circovirus, has been identified in individuals with clinical symptoms.ObjectivesThe prevalence of porcine circovirus 2 and porcine circovirus 3 and the confirmation of diagnosis of this emerging viral disease have not been fully studied yet. Therefore, the objective of the present study was to investigate the prevalence of porcine circovirus 2 and porcine circovirus 3 in slaughtered pigs and wild boars in Korea between 2018 and 2019.MethodsLungs and hilar lymph nodes of healthy pigs slaughtered in slaughterhouses and captured wild pigs were collected, and viruses were detected by multiplex quantitative polymerase chain reaction and two staining methods (in situ hybridization and immunohistochemistry) to confirm the presence of porcine circovirus 2 and porcine circovirus 3.ResultsPositive rates of porcine circovirus 2 in lungs and hilar lymph nodes were 78.1% (75/96) and 89.5% (86/96) in slaughtered pigs, respectively. They were 18.0% (30/167) and 46.3% (24/55) in wild boars, respectively. Positive rates of porcine circovirus 3 in lungs and hilar lymph nodes were 30.2% (29/96) and 13.5% (13/96) in slaughtered pigs, respectively. They were 4.2% (7/167) and 5.5% (3/55) in wild boars, respectively. At the farm level, positive rates of porcine circovirus 2 and porcine circovirus 3 were 97.9% (47/48) and 54.2% (26/48), respectively. Positive rates of porcine circovirus 2 and porcine circovirus 3 decreased in spring. Immunohistochemistry and in situ hybridization confirmed the presence of porcine circovirus 2 and porcine circovirus 3 in lungs, but not porcine circovirus 3 in the hilar lymph nodes.ConclusionThese results suggest that the prevalence of porcine circovirus 2 and porcine circovirus 3 might vary depending on the season and the type of sample. Wild boars might play a role in the epidemiology of porcine circovirus 2 and porcine circovirus 3 in South Korea. Continuous surveillance and further study are needed for this emerging disease.

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Section: ) the Discrepancy Of The Target Cells Between Pcv2 And Pcv3mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…have also been detected in wild boars in many countries including Germany, China, Brazil, Spain, Italy, and South Korea (Dhandapani et al, 2021;Sirisereewan et al, 2022;Song et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of porcine circovirus type 2 and type 3 in slaughtered pigs and wild boars in Korea

Park,

Kim,

Jeong

et al. 2023

Veterinary Medicine & Sci

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“…In 2016, Palinski et al [4] was the rst to discover PCV3 through metagenomic analysis in diseased sows with Porcine Dermatitis and Nephropathy Syndrome (PNDS) and in aborted foetuses. Since then, numerous reports detecting PCV3 have been published in many countries, including the United States, China, Brazil, Italy, South Korea, Thailand, Spain, Denmark, Germany, Sweden, and Poland, indicating the rapid global spread of the virus [5][6][7][8][9][10][11][12]. In addition to being detected in pigs, PCV3 has also been identi ed in canids and other mammalian species [13,14].…”

Section: Introductionmentioning

confidence: 99%

Rescue and identification of recombinant Porcine Circovirus Type 3

Zhang,

Cai,

Zhu

et al. 2024

Preprint

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PCV3 is prevalent and causes many forms of swine diseases worldwide. To date, PCV3 isolation has been unsuccessful. Therefore, obtaining PCV3 and studying its biological traits are urgently needed. In the present study, recombinant PCV3 (rPCV3) was successfully generated, and it’s biologically characterization was performed. The genome sequence of PCV3 was optimized, cloned and inserted into the pBluescript SK vector. PK-15 cells transfected with the recombinant plasmid were serially passaged and characterized. The obtained rPCV3 was purified through sucrose density gradient centrifugation and ion exchange chromatography and observed via Transmission Electron Microscope (TEM). Absolute qPCR was used to determine PCV3 viral load. PK-15 cells were treated with nocodazole to determine the relationship between rPCV3 proliferation and mitosis. Especially, PK-15 cell infected with rPCV3 was compared with that infected with PCV3 positive tissues (wPCV3). Specific fluorescence in the nuclei, brownish-red puncta on cell monolayers, and target bands in NC membrane were observed in transfected PK-15 cells. TEM showed that the particle diameter of rPCV3 was approximately 20 nm. rPCV3 was continuously passaged for up to 25 passages with a progressive decrease in viral load. Nocodazole experiments demonstrated that rPCV3 proliferation was dependent on cell mitosis. Cytoplasmic fluorescence was observed whether the cells were infected with rPCV3 or wPCV3; importantly, copy numbers decreased in a time-dependent manner. Our study is the first to observe PCV3 particles via TEM. We revealed that both rPCV3 and wPCV3 cannot enter the PK-15 cell nucleus, which may explain why virus isolation was unsuccessful in these cells.

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Detection of Porcine circovirus 3 from captured wild boars in Korea

Dhandapani

Yoon

Noh

et al. 2021

Veterinary Medicine & Sci

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Porcine circovirus 3 (PCV3) is a newly discovered ssDNA virus. The virus was first reported in pigs suffering from several clinical syndromes, including porcine dermatitis and nephropathy syndrome, reproductive disorders, respiratory disease and myocarditis. PCV3 was recently reported in wild boars with high prevalence as well. In this study, 266 wild boar anal swab, feces, nasal swab and whole blood samples were collected from three mainland provinces and one island province (Chungbuk, Gangwon, Gyeonggi, Jeju) of South Korea between 2019 and 2020 including 119 from male, 142 from female and 5 undetermined. PCV3 was diagnosed targeting conserved rep (replication associated protein) gene region using Direct PCR and sequencing. Out of 266 tested samples, 15 were positive for PCV3 with detection frequency at 5.6%. Among 266 samples tested, we obtained 14 partial rep gene sequences and one complete genome sequence of PCV3 with a genome size of 2000nt. Here we present the evidence of PCV3 circulation in Korean wild boars.

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Detection of Porcine circovirus 3 from captured wild boars in Korea

Cited by 8 publications

References 31 publications

Prevalence of porcine circovirus type 2 and type 3 in slaughtered pigs and wild boars in Korea

Prevalence of porcine circovirus type 2 and type 3 in slaughtered pigs and wild boars in Korea

Rescue and identification of recombinant Porcine Circovirus Type 3

Detection of Porcine circovirus 3 from captured wild boars in Korea

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