Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs

Uddin, Muhammad Jasim; Duy, Do Ngoc; Çınar, Mehmet Ulaş; Tesfaye, Dawit; Tholen, Ernst; Juengst, H.; Looft, Christian; Schellander, K.

doi:10.1186/1471-2156-12-62

Cited by 16 publications

(11 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, TC showed a highly significant phenotypic correlation with TG, LDL-C and HDL-C, whereas TG showed a significant phenotypic correlation with serum levels of HDL-C and LDL-C. Furthermore, there was a highly significantly positive correlation between serum levels of LDL-C and HDL-C. Our results are very similar to the phenotypic correlations among serum lipids levels reported in a Duroc 9 Pietrain cross-bred population and in Duroc pigs (Rauw et al 2007;Uddin et al 2011).…”

supporting

confidence: 92%

“…Descriptive statistics and correlations among these traits were calculated by the PROC MEANS and PROC CORR procedures of SAS software package version 9.2 (SAS Institute). The concentrations of blood lipid indicators were in the expected range (Table S1) and differed according to the age (Uddin et al 2011;Muñoz et al 2012). Phenotypic correlations among serum lipid concentrations at 45 days and 300 days are shown in Table S1.…”

mentioning

confidence: 96%

“…mcw.edu/) and mice (http://www.informatics.jax.org/), but few studies have investigated QTL for serum lipids in pigs. Recently, a number of QTLs have been mapped for porcine blood lipids using whole genome-linkage analysis (Gallardo et al 2008;Uddin et al 2011) and the genome-wide association approach (Chen et al 2013). To date, 43 QTL for total cholesterol (TC), 21 for high-density lipoprotein cholesterol (HDL-C), 68 for low-density lipoprotein cholesterol (LDL-C), 22 for HDL-C/LDL-C and 25 for triglycerides (TG) have been reported in the pig QTL database (http:// www.animalgenome.org/cgi-bin/QTLdb/SS/index).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Functional and association studies of the cholesteryl ester transfer protein (CETP) gene in a Wannan Black pig model

Ding

Zhang

et al. 2015

Animal Genetics

View full text Add to dashboard Cite

Some polymorphisms of the human CETP gene are causally and significantly associated with serum lipids levels; however, the information regarding this gene in pigs is sparse. To evaluate the effects of CETP on blood lipid traits and fat deposition in pig, porcine CETP tissue expression patterns were observed by quantitative real-time polymerase chain reaction (qPCR) first. High expression was detected in liver, spleen, gluteus medius (GM) muscle and backfat. A de novo polymorphism (AF333037:g.795C>T) in the intron 1 region of porcine CETP was identified. This polymorphism was further genotyped by direct sequencing of the PCR products of 390 Wannan Black pigs, a Chinese native breed population. Association analyses at 45 and 300 days of age revealed highly significant associations between CETP genotypes and serum lipid traits. Furthermore, this polymorphism was proved to be associated with differences in liver CETP mRNA levels: pigs at 300 days of age with the TT genotype had higher levels than did those with other genotypes (P = 0.021). Additionally, analysis at 300 days of age showed that GM CETP mRNA expression correlated positively with serum lipids levels as well as with carcass backfat thickness and intramuscular fat content in GM. These results indicate that CETP is involved in serum, adipose and muscle lipid metabolism in pigs. The mechanisms underlying such relationships and their functional implications are worthy of further research.

show abstract

supporting

confidence: 92%

mentioning

confidence: 96%

mentioning

confidence: 99%

See 1 more Smart Citation

Functional and association studies of the cholesteryl ester transfer protein (CETP) gene in a Wannan Black pig model

Ding

Zhang

et al. 2015

Animal Genetics

View full text Add to dashboard Cite

show abstract

“…In the Yorkshire pigs, e.g., several micro-satellite markers were significantly associated with average daily gain and backfat thickness (Kim et al, 2006). In a Duroc × Pietrain F2 population, several QTLs were significantly associated with serum lipids, including a large QTL on SSC1 (Uddin et al, 2011). This has also been reported to be in association with average daily gain and carcass composition in the same population (Liu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

An F2 Pig Resource Population as a Model for Genetic Studies of Obesity and Obesity-Related Diseases in Humans: Design and Genetic Parameters

et al. 2013

View full text Add to dashboard Cite

Obesity is a rising worldwide public health problem. Difficulties to precisely measure various obesity traits and the genetic heterogeneity in human have been major impediments to completely disentangle genetic factors causing obesity. The pig is a relevant model for studying human obesity and obesity-related (OOR) traits. Using founder breeds divergent with respect to obesity traits we have created an F2 pig resource population (454 pigs), which has been intensively phenotyped for 36 OOR traits. The main rationale for our study is to characterize the genetic architecture of OOR traits in the F2 pig design, by estimating heritabilities, genetic, and phenotypic correlations using mixed- and multi-trait BLUP animal models. Our analyses revealed high coefficients of variation (15–42%) and moderate to high heritabilities (0.22–0.81) in fatness traits, showing large phenotypic and genetic variation in the F2 population, respectively. This fulfills the purpose of creating a resource population divergent for OOR traits. Strong genetic correlations were found between weight and lean mass at dual-energy x-ray absorptiometry scanning (0.56–0.97). Weight and conformation also showed strong genetic correlations with slaughter traits (e.g., rg between abdominal circumference and leaf fat at slaughtering: 0.66). Genetic correlations between fat-related traits and the glucose level vary between 0.35 and 0.74 and show a strong correlation between adipose tissue and impaired glucose metabolism. Our power calculations showed a minimum of 80% power for QTL detection for all phenotypes. We revealed genetic correlations at population level, for the first time, for several difficult to measure and novel OOR traits and diseases. The results underpin the potential of the established F2 pig resource population for further genomic, systems genetics, and functional investigations to unravel the genetic background of OOR traits.

show abstract

“…Pig chromosome (SSC) 2 has been reported to harbor several QTL for HDL‐C (42.0–55.0 c M ; Uddin et al . ), LDL‐C (67.0 c M ; Chen et al . ), TG (107.9–128.2 c M ; Uddin et al .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the causality of the low‐density lipoprotein receptor gene (LDLR) for serum lipids in pigs

et al. 2014

View full text Add to dashboard Cite

A significant quantitative trait locus (QTL) for low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) was identified around the LDLR gene on chromosome 2 (SSC2) in a White Duroc × Erhualian F2 resource population and Sutai pigs in our previous study. However, in previous reports, the causality of LDLR with serum lipids is controversial in pigs. To systematically assess the causality of LDLR with serum lipids, association analyses were successively performed in three populations: Sutai pigs, a White Duroc × Erhualian F2 resource population and a Duroc × (Landrace × Large White) population. We first performed a haplotype-based association study with 60K SNP genotyping data and evidenced the significant association with LDL-C and TC around the LDLR gene region. We also found that there is more than one QTL for LDL-C and TC on SSC2. Then, we evaluated the causalities of two missense mutations, c.1812C>T and c.1520A>G, with LDL-C and TC. We revealed that the c.1812C>T SNP showed the strongest association with LDL-C (P = 5.40 × 10(-11) ) and TC (P = 3.64 × 10(-8) ) and explained all the QTL effect in Sutai pigs. Haplotype analysis found that two missense SNPs locate within a 1.93-Mb haplotype block. One major haplotype showed the strongest significant association with LDL-C (P = 4.62 × 10(-18) ) and TC (P = 1.06 × 10(-9) ). However, the c.1812C>T SNP was not identified in the White Duroc × Erhualian intercross, and the association of c.1520A>G with both LDL-C and TC did not achieve significance in this F2 population, suggesting population heterogeneity. Both missense mutations were identified in the Duroc × (Landrace × Large White) population and showed significant associations with LDL-C and TC. Our data give evidence that the LDLR gene should be a candidate causative gene for LDL-C and TC in pigs, but heterogeneity exists in different populations.

show abstract

Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs

Cited by 16 publications

References 80 publications

Functional and association studies of the cholesteryl ester transfer protein (CETP) gene in a Wannan Black pig model

Functional and association studies of the cholesteryl ester transfer protein (CETP) gene in a Wannan Black pig model

An F2 Pig Resource Population as a Model for Genetic Studies of Obesity and Obesity-Related Diseases in Humans: Design and Genetic Parameters

Evaluation of the causality of the low‐density lipoprotein receptor gene (LDLR) for serum lipids in pigs

Contact Info

Product

Resources

About