Detection of Rhodococcus equi by PCR from foals and determination of antimicrobial susceptibility

Kırkan, Şükrü; Parın, Uğur; DOLGUN, Hafize Tuğba YÜKSEL; ORAL, Elif Oya

doi:10.3906/vet-2011-109

Cited by 3 publications

(2 citation statements)

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“…In recent years, the Polymerase Chain Reaction (PCR) detection approach based on molecular biology and Enzyme-linked Immunosorbent Assay (ELISA) detection based on immunology have been widely studied. Real-time quantitative PCR (qPCR) is a nucleic acid detection method widely used in laboratories at present, with high sensitivity, strong specificity and quantitative detection [ 7 , 8 ]. However, the instrument is expensive and requires specialist personnel to operate [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System

Yang,

Kong,

Yang

et al. 2024

IJMS

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Listeria monocytogenes (L. monocytogenes) is a food-borne pathogenic bacteria that frequently contaminates animal-derived food and low-temperature preserved food. Listeriosis caused by its infection has a high mortality rate and poses a serious threat to human health. Therefore, it is crucial to establish a sensitive, rapid and easy-to-operate technique. In this study, a Recombinase Aided Amplification (RAA) assisted CRISPR/Cas12a (RAA-CRISPR/Cas12a) fluorescence platform was established for highly sensitive nucleic acid detection of L. monocytogenes. The established RAA-CRISPR/Cas12a showed high sensitivity and high specificity, with the sensitivity of 350 CFU/mL and 5.4 × 10−3 ng/μL for pure bacterial solution and genomic DNA, and good specificity for 5 strains of Listeria spp. and 14 strains of other common pathogenic bacteria. L. monocytogenes could be detected at an initial concentration of 2.3 CFU/25g within 2 h of enriching the beef in the food matrix, and this method could be applied to food samples that were easily contaminated with L. monocytogenes The results of RAA-CRISPR/Cas12a could be observed in 5 min, while the amplification was completed in 20–30 min. The speed and sensitivity of RAA-CRISPR/Cas12a were significantly higher than that of the national standard method. In conclusion, the RAA-CRISPR/Cas12a system established in this study has new application potential in the diagnosis of food-borne pathogens.

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Section: Introductionmentioning

confidence: 99%

“…used in laboratories at present, with high sensitivity, strong specificity and quantitative detection [7,8]. However, the instrument is expensive and requires specialist personnel to operate [9].…”

Section: Introductionmentioning

confidence: 99%

Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System

Yang,

Kong,

Yang

et al. 2024

IJMS

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Antimicrobial susceptibility of Rhodococcus equi strains isolated from foals in Chile

Zúñiga

Badillo

Abalos

et al. 2023

World J Microbiol Biotechnol

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Rhodococcus equi is responsible for foal pneumonia worldwide, with a significant economic impact on the production and breeding of horses. In Chile, the first case was reported in 2000, and since then, its incidence has been increasing. Distinctive characteristics of R. equi as an intracellular pathogen in macrophages, emergence of virulence plasmids encoding surface lipoprotein antigens, and appearance of antibiotic resistance against macrolides and rifampicin have significantly complicated the treatment of R. equi pneumonia in foals. Therefore, in vitro susceptibility studies of first-line and newer antibiotics against R. equi are the first step to establishing effective treatments and optimizing new therapeutic options. The aim of the present study is to determine the susceptibility profile of fourteen strains of R. equi isolated from foals in Chile to several antibiotics of the macrolide group including azithromycin, amikacin, tildipirosin and gamithromycin as well as others such as rifampicin, doxycycline and ceftiofur. Identification of R. equi in collected isolates from foals in Chile has been performed by CAMP test and PCR based on detecting of the gene encoding the 16 S rRNA. The presence of genes encoding virulence plasmids was also determined using PCR. Results obtained have demonstrated presence of virulent R. equi strains in Chile. In vitro susceptibility pattern to different antibiotics has shown better results for doxycycline and rifampicin similar to previous studies performed. Current macrolides have been evaluated in order to consider alternative treatment options in a context of emerging resistance to classic macrolides and rifampicin, obtaining better results with gamithromycin (MIC range of 0.125 to 128 mg/ml) than with tildipirosin (MIC range of 16 to 128 mg/ml). An adequate diagnosis of bacterial susceptibility based on antibiograms is necessary to treat the Rhodococcus equi infection in foals.

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Pangenomic and functional investigations for dormancy and biodegradation features of an organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9

Wang

Blaustein

et al. 2022

Science of The Total Environment

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Detection of Rhodococcus equi by PCR from foals and determination of antimicrobial susceptibility

Cited by 3 publications

References 0 publications

Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System

Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System

Antimicrobial susceptibility of Rhodococcus equi strains isolated from foals in Chile

Pangenomic and functional investigations for dormancy and biodegradation features of an organic pollutant-degrading bacterium Rhodococcus biphenylivorans TG9

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