Detection of virulence genes of enterotoxigenic Staphylococcus aureus isolated from chicken meat cuts by using PCR

Hassanen, Fatin; Shaltout, Fahim; Amani, Samad; Maarouf, Ahmed Afifi; Rasha, A

doi:10.21608/bvmj.2017.30515

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…On the other hand, higher incidence was reported by Hassanen et al (2017), Mahmoud andHamouda-Seham (2006) and Elshraway et al (2018) with 22.5, 51.5 and 53% respectively of the examined chicken fillet samples. Moreover, Saad et al (2018), Hassanen et al (2017) and Elshraway et al (2018) reported a percentage of 80, 27.5 and 45% respectively. In MDM samples nearly the same results were obtained by Kozačinski et al (2006) who detected coagulase positive S. aureus in 30.3% of the examined MDM samples.…”

Section: Resultsmentioning

confidence: 96%

Incidence of Staphylococcus aureus and its Enterotoxins in Chicken Meat and its Products

2020

Int J Vet Sci

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Chicken meat are being widely consumed as they contain high protein and a healthier unsaturated fat type. Chicken burger represent a consumer palatable chicken product. Both chicken and its products are liable to different types of contamination during their preparation and processing. Contamination by S. aureus and its enterotoxins poses a major public health hazard to chicken meat consumes. During this study 100 different samples of chicken fillet, deboned thigh, wing, mechanically deboned meat (MDM) and chicken burger (20 each) was collected from market and investigated for their S. aureus count and ability of the isolated strains to produce enterotoxins using conventional plating and isolation technique as well as using SET-RPLA toxin detection kit. Results revealed that mean values of S. aureus count in all samples exceeded the permissible limits and hence being unacceptable. MDM isolated exhibited staphylococcal enterotoxins (SEs) production of three different types SEA, SEC and SED. Meanwhile chicken burger S. aureus isolates produced only SEA and SEC enterotoxins. While isolated S. aureus from chicken fillet and deboned thigh didn’t exhibit any enterotoxin production activity. It’s recommended to follow the hygienic practices during different processing stages to avoid the risk of S. aureus and its enterotoxins.

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Section: Resultsmentioning

confidence: 96%

Incidence of Staphylococcus aureus and its Enterotoxins in Chicken Meat and its Products

2020

Int J Vet Sci

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Detection of Staphylococcal Enterotoxins A and E and Methicillin Resistance in Staphylococcus aureus Strains From Moroccan Broiler Chicken Meat

Nacer,

Nassik,

El Ftouhy

et al. 2024

International Journal of Food Science

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Foodborne epidemics have become a serious public health emergency worldwide. Foods of animal origin, in particular chicken meat, are considered to be potential vectors of pathogenic bacteria, particularly Staphylococcus aureus. This bacterium can be resistant in the form of methicillin‐resistant S. aureus (MRSA) or produce enterotoxins leading to food poisoning when ingested. This study is aimed at exploring the virulence genes in S. aureus responsible for producing enterotoxins (staphylococcal enterotoxin [SE] A [sea] and SE E [see]) and determining the prevalence of MRSA in raw broiler meat in the Casa‐Rabat region in Morocco. A quantitative (q) PCR (qPCR) assay, using specific primers for S. aureus (nuc) confirmation and detection of enterotoxin genes (sea and see), as well as the methicillin‐resistant gene (mecA), was employed. Our findings indicated that all tested strains were positively identified as S. aureus. Among them, one isolate (1/54) tested positive for the see gene (1.85%), while none carried the sea gene. Furthermore, the mecA gene, indicative of MRSA, was present in 12/54 of the isolates (22.22%). The potential presence of MRSA in Moroccan poultry meat underscores a public health risk. Thus, stringent measures are imperative to curtail the contamination and proliferation of this bacterium during the slaughtering process, underscoring the importance of continuing research into the prevalence of MRSA colonization among poultry slaughterhouse personnel.

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Bacteriological Profile of Frozen Chicken Meat Cuts at Qalubiya Governorate Markets

Hassanin

Shaltout

Maarouf

et al. 2020

Benha Veterinary Medical Journal

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The study was conducted on 100 random samples of frozen chicken meat cuts represented by chicken wings, drumstick, thigh and breast (25 of each) purchased from different markets at Qalubiya Governorate, Egypt. The collected samples were bacteriological examination to investigate the bacteriological quality. The obtained results cleared that the mean values of APC, psychrotrophs; coliforms and S. aureus counts (CFU/g) of the wing samples were the most contaminated with such bacterial groups followed by drumstick, thigh, and breast samples. Further, bacteriological isolation of some food poisoning bacteria revealed detection of E. coli and coagulase positive S. aureus (CoPSA) in 11, and 21% of the examined samples, respectively. On the other hand, Salmonella species could not be detected in any of the examined samples. Concerning detection and typing of some enterotoxigenic CoPSA, 5 isolates were randomly examined using SET-RPLA test and the results indicated detection of Staphylococcal enterotoxin A in 3 isolates (60%), while Staphylococcal enterotoxins C and D were detected in one isolate (20% of each). Therefore, the sources and public health significance as well as trials for control of such serious food poisoning bacteria were discussed.

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Detection of virulence genes of enterotoxigenic Staphylococcus aureus isolated from chicken meat cuts by using PCR

Cited by 3 publications

References 16 publications

Incidence of Staphylococcus aureus and its Enterotoxins in Chicken Meat and its Products

Incidence of Staphylococcus aureus and its Enterotoxins in Chicken Meat and its Products

Detection of Staphylococcal Enterotoxins A and E and Methicillin Resistance in Staphylococcus aureus Strains From Moroccan Broiler Chicken Meat

Bacteriological Profile of Frozen Chicken Meat Cuts at Qalubiya Governorate Markets

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