Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul

Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase-Polymerase Chain Reaction (RT-PCR).

Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia

“…Results of detection with PCR against control and positive samples ORSV showed fragments of DNA amplification sized ± 474 bp dan ± 599 bp which is CP gene and rbcL gene, as reported [6,7,8,9,10].…”

“…Orchid is the ornamental plant have the highest demand in the market. Infection of pests and disease is still a major obstacle in the attempt of cultivating orchids [6]. Viral infection is the most important limiting factor because it can lower the quality of the plants [12].…”

“…Information about orchid disease caused by ORSV infection in Indonesia is very limited. Research on the detection and characterization of ORSV in Indonesia has been reported [6,7,8,9]. Biotic stress affects issues declining ability of photosynthesis.…”

Penerapan Teknologi Kesehatan Tepat Guna di Era Industri 4.0 dalam Perlindungan Anggrek Alam Terhadap Infeksi Penyakit di Kebun Raya

“…Hasil uji serologis membuktikan bahwa terdapat infeksi campuran, yaitu Odontoglossum ringspot virus (ORSV) dan Cymbidium mosaic virus (CymMV). Keduanya termasuk virus penting karena dilaporkan memiliki persebaran penyakit yang luas di dunia termasuk sudah sampai di Indonesia (Mahfut & Setiadi Daryono, 2014;Mahfut & Daryono, 2019;Mahfut, 2019;Mahfut et al, 2019aMahfut et al, , 2019bMahfut, 2020aMahfut, , 2020b Upaya perlindungan terhadap infeksi penyakit harus dilakukan agar keberadaaan anggrek alam di Kebun Raya Liwa terjaga kelestariannya. Berdasarkan informasi langsung dari Kepala PLT Kebun Raya Liwa, Bapak Sukimin, S.Ip.,M.M.…”

Identification of NativeDendrobiumBased on Morphological and Anatomical Characters in Liwa Botanical Garden

“…For this reason, the existence of the Liwa Botanical Garden is expected to guarantee the preservation of natural orchid species that can be utilized sustainably. However, until now there are several types of natural orchids that are not known with certainty what natural orchid species exist in the Liwa Botanical Garden [7][8][9][10][11].…”