Detergent-compatible fungal cellulases

Niyonzima, François N.

doi:10.1007/s12223-020-00838-w

Cited by 19 publications

(7 citation statements)

References 60 publications

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“…The recognition of filamentous fungi with hydrolytic enzymatic activity suggests that they are preferred sources for the industry, due to superior metabolic versatility ( Su et al 2012 ). Fungal enzymes are used in biofuel production processes, bioremediation of organic contaminants, for detergent production, antibiotic production, bleaching of textile fabrics, juice clarification, removal of leather aromas and paper production ( El-Sawah et al 2021 , Füting et al 2021 , Hyde et al 2019 , Kirk et al 2002 , Meyer et al 2020 , Molina-Gutiérrez et al 2021 , Niyonzima 2020 , Singh et al 2021 , Sudeep et al 2020 , Syedd-León et al 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Gómez-Vázquez,

Sánchez Roque,

Ibáñez-Duharte

et al. 2024

BDJ

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Filamentous fungi, microorganisms that develop and are located in different habitats, are considered important producers of enzymes and metabolites with potential for the biotechnology industry. The objective of this work was to isolate and identify filamentous fungi that grow in used oil. Two fungal species were characterised through their morphology and molecular identification. The DNA of each extracted strain was amplified by PCR using primers ITS1 and ITS4, obtaining sequences that were later in GenBank (NCBI). A white coloured strain (HB) with a cottony, white, hyaline morphology and irregular borders was observed; so too, a brown colony (HC) with a sandy surface, a well-defined border of beige colour in early growth until it became a dark brown colour. The identity result by homology of the sequences in the BLASTn database was 100% and 99.55%, indicating that they correspond to Cladosporium tenuissimum and Fomitopsis meliae, respectively. Finally, the results in lipolytic activity show greater potential for Fomitopsis meliae with 0.61 U/l in residual oil. Thus, it is important to highlight the potential of this type of waste to favour the prospection of microorganisms for a sustainable alternative for future studies of biological conversion.

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Section: Resultsmentioning

confidence: 99%

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Gómez-Vázquez,

Sánchez Roque,

Ibáñez-Duharte

et al. 2024

BDJ

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“…The need for alkaline biocatalysts capable of functioning at ambient and low temperatures in the detergent industry is increasing. This is necessary to maintain fabric quality and reduce energy costs, especially since the majority of available bacterial and fungal cellulases have preferred temperatures between 40 and 80°C (Niyonzima, 2019 , 2021 ). This evidence Cel240 structure as potential to tolerate a very broad range of temperatures and may be an alternative glucanase for application at processes that require low temperatures.…”

Section: Resultsmentioning

confidence: 99%

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Oliva,

Zervas,

Stougaard

et al. 2024

Microbial Biotechnology

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Microbial communities from extreme environments are largely understudied, but are essential as producers of metabolites, including enzymes, for industrial processes. As cultivation of most microorganisms remains a challenge, culture‐independent approaches for enzyme discovery in the form of metagenomics to analyse the genetic potential of a community are rapidly becoming the way forward. This study focused on analysing a metagenome from the cold and alkaline ikaite columns in Greenland, identifying 282 open reading frames (ORFs) that encoded putative carbohydrate‐modifying enzymes with potential applications in, for example detergents and other processes where activity at low temperature and high pH is desired. Seventeen selected ORFs, representing eight enzyme families were synthesized and expressed in two host organisms, Escherichia coli and Aliivibrio wodanis. Aliivibrio wodanis demonstrated expression of a more diverse range of enzyme classes compared to E. coli, emphasizing the importance of alternative expression systems for enzymes from extremophilic microorganisms. To demonstrate the validity of the screening strategy, we chose a recombinantly expressed cellulolytic enzyme from the metagenome for further characterization. The enzyme, Cel240, exhibited close to 40% of its relative activity at low temperatures (4°C) and demonstrated endoglucanase characteristics, with a preference for cellulose substrates. Despite low sequence similarity with known enzymes, computational analysis and structural modelling confirmed its cellulase‐family affiliation. Cel240 displayed activity at low temperatures and good stability at 25°C, activity at alkaline pH and increased activity in the presence of CaCl2, making it a promising candidate for detergent and washing industry applications.

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“…Cellulase has been widely used in many industrial fields, such as food processing, medical materials, detergent processing, etc. [32][33][34]. Generally, endoglucanase (EG), exoglucanase (CBH) and β-glucosidase (BG) are the major three components for cellulose degradation [35].…”

Section: Typical Hydrolases and Microorganisms For Lignocellulose Degradation 21 Hydrolases Responsible For Lignocellulose Degradationmentioning

confidence: 99%

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

et al. 2021

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Lignocellulose is a kind of renewable bioresource containing abundant polysaccharides, which can be used for biochemicals and biofuels production. However, the complex structure hinders the final efficiency of lignocellulosic biorefinery. This review comprehensively summarizes the hydrolases and typical microorganisms for lignocellulosic degradation. Moreover, the commonly used bioprocesses for lignocellulosic biorefinery are also discussed, including separated hydrolysis and fermentation, simultaneous saccharification and fermentation and consolidated bioprocessing. Among these methods, construction of microbial co-culturing systems via consolidated bioprocessing is regarded as a potential strategy to efficiently produce biochemicals and biofuels, providing theoretical direction for constructing efficient and stable biorefinery process system in the future.

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Detergent-compatible fungal cellulases

Cited by 19 publications

References 60 publications

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Metagenomic exploration of cold‐active enzymes for detergent applications: Characterization of a novel, cold‐active and alkali‐stable GH8 endoglucanase from ikaite columns in SW Greenland

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

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