Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Ribeiro, Vanessa da Silva; Feliciano, Nágilla Daliane; Gonzaga, Henrique Tomaz; Costa, Idessânia Nazareth; Gonçalves-Pires, M.R.F.; Ueta, Marlene Tiduko; Costa-Cruz, Júlia Maria

doi:10.1016/j.imlet.2010.08.011

Cited by 20 publications

(19 citation statements)

References 34 publications

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“…Several studies demonstrated that anti-L3 antibodies, using homologous or heterologous antigens, can be detected in more than 80% of coprologically positive patients [6][7][8][9][10]. Those data showed that enzyme-linked immunosorbent assay (ELISA) protocols are helpful, with high sensitivity and specificity values, although distinction between infections with S. stercoralis from serologically positive cases with negative coproparasitological tests cannot be successfully established [3].…”

Section: Introductionmentioning

confidence: 99%

IgG avidity in differential serodiagnosis of human strongyloidiasis active infection

et al. 2011

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IgG avidity assays have been developed for several parasitic diseases although there are no researches focused in strongyloidiasis diagnosis. Definitive diagnosis of strongyloidiasis is based on the presence of Strongyloides larvae in stool, but majority of cases involve low and irregular larval output. While limitations of serological assays for strongyloidiasis are well known, characteristics of persons who are misdiagnosed based on negative coproparasitological tests have been little explored. The aim of the present study was to evaluate the use of IgG avidity to detect patients with active strongyloidiasis and to characterize sources of disagreement between serology and coproparasitology. A total of 80 serum samples was analyzed, 40 from patients with Strongyloides larvae in stool (G1) and 40 from individuals with negative coproparasitology, but positive serology (G2). Serum samples were analyzed in an indirect IgG avidity ELISA using urea 6M in serial double dilutions from 1:80 to 1:2560. Avidity index (AI) was calculated to each serum dilution and analyzed as screening AI (serum dilution of 1:160) or mean AI of different serum dilutions that had a positive result. Statistical analyzes were performed by Mann-Whitney's (U) and Fisher's exact tests. At screening dilution, median of AI was 68% in G1 and 88% in G2 (P<0.0001), whereas median of mean AI in G1 was 72% and in G2 94% (P<0.0001), but there was no significant differences between both AI in each patient group. A cut off value established at AI of 75% demonstrated a significant difference between groups, with G1 sera showing AI<75% and G2 sera with AI>75% (P<0.0001). In conclusion, IgG avidity assays may distinguish active infection with Strongyloides stercoralis from suspect or serologically false positive cases.

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Section: Introductionmentioning

confidence: 99%

IgG avidity in differential serodiagnosis of human strongyloidiasis active infection

et al. 2011

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“…The detection of IgA anti- S. stercoralis can also aid in the diagnosis of strongyloidiasis [107], especially in patients without excretion of rhabditoid larvae in feces. Due to the immunomodulation of IgA during infections, the results of stool tests are usually negative [63].…”

Section: S Stercoralis Diagnosis and Treatmentmentioning

confidence: 99%

Strongyloides stercoralisInfection in Alcoholic Patients

Teixeira

Pacheco

Souza

et al. 2016

BioMed Research International

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The course of Strongyloides stercoralis infection is usually asymptomatic with a low discharge of rhabditoid larva in feces. However, the deleterious effects of alcohol consumption seem to enhance the susceptibility to infection, as shown by a fivefold higher strongyloidiasis frequency in alcoholics than in nonalcoholics. Moreover, the association between S. stercoralis infection and alcoholism presents a risk for hyperinfection and severe strongyloidiasis. There are several possible mechanisms for the disruption of the host-parasite equilibrium in ethanol-addicted patients with chronic strongyloidiasis. One explanation is that chronic ethanol intake stimulates the hypothalamic-pituitary-adrenal (HPA) axis to produce excessive levels of endogenous cortisol, which in turn can lead to a deficiency in type 2 T helper cells (Th2) protective response, and also to mimic the parasite hormone ecdysone, which promotes the transformation of rhabditiform larvae to filariform larvae, leading to autoinfection. Therefore, when untreated, alcoholic patients are continuously infected by this autoinfection mechanism. Thus, the early diagnosis of strongyloidiasis and treatment can prevent serious forms of hyperinfection in ethanol abusers.

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“…Immunodiagnosis of strongyloidiasis using saliva samples 10,11 can effectively contribute to the early detection of this infection so as to avoid cases of coinfection.…”

Section: Introductionmentioning

confidence: 99%

Strongyloides‐specific IgA, IgG and IgG immune complex profile in patients with pulmonary tuberculosis

Bosqui

Silva

Gonzaga

et al. 2020

Parasite Immunology

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Aims To describe an anti‐Strongyloides IgA, IgG and IgG immune complex antibody response profile in patients with pulmonary tuberculosis. Methods and Results Saliva and serum samples were collected from 100 individuals: group I, 50 apparently healthy individuals; and group II, 50 pulmonary tuberculosis patients. The IgA, IgG and IgG immune complex detection were carried out via an ELISA immunoenzymatic test. Optical density medians in saliva samples of IgA antibody (median of 7.21) and IgG‐IC (median of 4.95) were significantly higher in tuberculosis group compared to control individuals (median IgA of 3.93 and IgG‐IC of 2.38). Conclusion This study presents antibody data to the field of pulmonary tuberculosis and strongyloidiasis coinfection, including saliva samples, and especially IgG immune complex detection.

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Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

Cited by 20 publications

References 34 publications

IgG avidity in differential serodiagnosis of human strongyloidiasis active infection

IgG avidity in differential serodiagnosis of human strongyloidiasis active infection

Strongyloides stercoralisInfection in Alcoholic Patients

Strongyloides‐specific IgA, IgG and IgG immune complex profile in patients with pulmonary tuberculosis

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