Determinação de compostos bioativos e capacidade sequestradora de radicais livres em extratos de folhas de Byrsonima crassifolia e Inga edulis

Sobrinho, Alessandra Carla Guimarães; Rogez, HervéLouis Ghislain; Nascimento, Victor Hugo Alves; Teixeira, Bruno José Brito; Dias, Aline de Lima; Souza, Jesus Nazareno Silva de

doi:10.34117/bjdv6n6-147

Cited by 4 publications

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“…It was observed that the polar, methanol and methanol: water fractions were able to extract higher concentrations of total phenolics. The literature reports that alcoholic extracts present higher levels of total phenolics, with ethanol and methanol as the best extractors compared to less polar and pure solvents (KUMAR et al, 2018;SOBRINHO et al, 2020). The results presented in table 1, were shared with the work of Sobrinho et al (2020), who used the same samples.…”

Section: Resultsmentioning

confidence: 54%

“…The literature reports that alcoholic extracts present higher levels of total phenolics, with ethanol and methanol as the best extractors compared to less polar and pure solvents (KUMAR et al, 2018;SOBRINHO et al, 2020). The results presented in table 1, were shared with the work of Sobrinho et al (2020), who used the same samples. The apolar fraction (ethyl acetate) was the one that extracted the least amount of total phenolics.…”

Section: Resultsmentioning

confidence: 54%

“…This may be directly related to the fact that this fraction presented higher content of total phenolics, in the two matrices, which are directly correlated with several biological properties, including antimicrobial activity (ANGELO; JORGE, 2007;SOUZA et al, 2008). Sobrinho et al (2020). It is clear that when these are fractioned, the MIC value decreased considerably, mainly for Gramnegative microorganisms (S. Typhimurium and E. coli).…”

Section: Resultsmentioning

confidence: 86%

“…The study of these Amazonian plants and their bioactive compounds can contribute to the discovery of new antimicrobial compounds, thus helping the region technological growth. Initial research has demonstrated the potential of bioactive compounds in relation to the biological activities from Byrsonima crassifolia and Inga edulis leaves (POMPEU et al, 2012;TAUCHEN et al, 2016;RODRIGUES et al, 2018;SOBRINHO et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Determination of Phenolic Compounds with Antimicrobial Activity of Byrsonima Crassifolia and Inga Edulis Leaves Extracts

et al. 2021

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The aim of this work was to determine the compounds with antimicrobial capacity from crude extracts and leaf fractions of Byrsonima crassifolia and Inga edulis. A solid-liquid extraction was performed using three solvent systems (acetate, methanol and methanol: water). The extracts and fractions were submitted to antimicrobial susceptibility tests for Gram-negative and Gram-positive bacteria by disk diffusion and Minimum Inhibitory Concentration (MIC) methods. A solid phase extraction (SPE) was performed to identify phenolic compounds for pre-concentration and pre-purification of the extracts and fractions that presented antimicrobial potential. The methanolic fraction was able to extract the highest total phenolic content and also presented the largest halos in the disk diffusion test. The minimum inhibitory concentration ranged from 125 to 500 μg/ml mL-1 and from 12.5 to 200 μg/ml mL-1 for Gram-negative and Gram-positive bacteria, respectively. After SPE, the crude extracts and fractions showed inhibition halos against all themicroorganisms tested at a lower concentration when compared to the standardized antibiotics. Gallic acid, ferric acid, myricetin and quercetin showed antimicrobial activity when tested alone. Gallic acid, catechin, epicatechin, ferrulic acid, quercetin, and other 4 major compounds were identified and quantified for Byrsonima crassifolia and 3 compounds for Inga edulis. Keywords: Vegetable Matrices. Antibiotics. Bioactive Compounds. Liquid Chromatography. Resumo O objetivo do presente estudo foi determinar compostos com capacidade antimicrobiana de extratos brutos e frações de folhas de Byrsonima crassifolia e de Inga edulis. Foi realizado uma extração sólido-líquido utilizando três sistemas de solventes (acetato, metanol e metanol:água). Os extratos e frações foram submetidos aos testes de suscetibilidade antimicrobiana para bactérias Gram-negativas e Gram-positivas através dos métodos de disco-difusão e Concentração Inibitória Mínima-CIM. Para identificação dos compostos fenólicos foi realizada Extração em Fase Sólida (SPE), para pré- concentração e pré-purificação dos extratos e frações que apresentaram potencial antimicrobiano. A fração metanólica conseguiu extrair maior conteúdo de fenólicos totais, e apresentou os maiores halos no teste de disco-difusão. A concentração inibitória mínima variou de 125 a 500 µgES mL-1 e de 12,5 a 200 µgES mL-1 para bactérias Gram-negativas e Gram-positivas, respectivamente. Após SPE, os extratos brutos e frações apresentaram halos de inibição frente a todos os micro-organismos testados em uma concentração menor que a dos antibióticos padronizados. O ácido gálico, ácido ferrúlico, miricetina e quercetina apresentaram atividade antimicrobiana quando testados isoladamente. Foram identificados e quantificados nos extratos brutos e frações ácido gálico, catequina, epicatequina, ácido ferrúlico, quercetina, além de outros 4 compostos majoritários para Byrsonima crassifolia e 3 compostos para Inga edulis. Palavras-chave: Matrizes Vegetais. Antibióticos. Compostos Bioativos. Cromatografia Líquida.

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Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Determination of Phenolic Compounds with Antimicrobial Activity of Byrsonima Crassifolia and Inga Edulis Leaves Extracts

et al. 2021

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“…Moreira-Araújo, et al, 2019;Guimarães Sobrinho, et al, 2020), desta forma, os antioxidantes naturais vêm sendo aplicados em busca de eficiência e atoxicidade, entretanto, em quantidades limitadas para não ocorrer alteração de maneira indesejada da característica sensorial do alimento(Rosa et al, 2013). Provindos de fontes vegetais variadas, estes antioxidantes em sua maioria são formados por compostos fenólicos, os quais apresentam um ou mais anéis aromáticos em sua estrutura, com características sensoriais marcantes no caso de temperos, como a sálvia, orégano, tomilho, entre outros, devendo ser levado em consideração sua contribuição ao sabor e aroma do produto(Brewer, 2011).…”

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Caracterização e aplicação de óleo de orégano como antioxidante natural em linguiça suína frescal

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Serafim

Souza

et al. 2020

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Extraction of antioxidant and antimicrobial compounds from Inga marginata Willd bark and pulp using different extraction techniques and phytochemical characterization

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Boeira

Weis

et al. 2023

Innovative Food Science & Emerging Technologies

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Determinação de compostos bioativos e capacidade sequestradora de radicais livres em extratos de folhas de Byrsonima crassifolia e Inga edulis

Cited by 4 publications

References 0 publications

Determination of Phenolic Compounds with Antimicrobial Activity of Byrsonima Crassifolia and Inga Edulis Leaves Extracts

Determination of Phenolic Compounds with Antimicrobial Activity of Byrsonima Crassifolia and Inga Edulis Leaves Extracts

Caracterização e aplicação de óleo de orégano como antioxidante natural em linguiça suína frescal

Extraction of antioxidant and antimicrobial compounds from Inga marginata Willd bark and pulp using different extraction techniques and phytochemical characterization

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