Determinación del perfil de resistencia antibiótica de Salmonella enterica aislada de cerdos faenados en un matadero de Lima, Perú

Ríos, Clara; Morales-Cauti, Siever; Vilca, L Miguel; Carhuallanqui, P Andrea; Ramos, D Daphne

doi:10.15381/rivep.v30i1.15701

Cited by 5 publications

(6 citation statements)

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“…Currently, the resistance spectra of the MDR strains of Salmonella serovars have been emerging in farm animals [ 24 ]. Because quinolones, chloramphenicol, aminoglycosides, and nitrofurantoin are exhaustively used as treatment and/or prophylactic in farm animals [ 68 , 70 , 71 , 72 , 73 , 74 ], constituting the leading resource of transmission on the emergence of resistant strains is reported.…”

Section: Discussionmentioning

confidence: 99%

WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

et al. 2022

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Salmonella Typhimurium is associated with foodborne diseases worldwide, including in Peru, and its emerging antibiotic resistance (AMR) is now a global public health problem. Therefore, country-specific monitoring of the AMR emergence is vital to control this pathogen, and in these aspects, whole genome sequence (WGS)—based approaches are better than gene-based analyses. Here, we performed the antimicrobial susceptibility test for ten widely used antibiotics and WGS-based various analyses of 90 S. Typhimurium isolates (human, animal, and environment) from 14 cities of Peru isolated from 2000 to 2017 to understand the lineage and antimicrobial resistance pattern of this pathogen in Peru. Our results suggest that the Peruvian isolates are of Typhimurium serovar and predominantly belong to sequence type ST19. Genomic diversity analyses indicate an open pan-genome, and at least ten lineages are circulating in Peru. A total of 48.8% and 31.0% of isolates are phenotypically and genotypically resistant to at least one antibiotic, while 12.0% are multi-drug resistant (MDR). Genotype–phenotype correlations for ten tested drugs show >80% accuracy, and >90% specificity. Sensitivity above 90% was only achieved for ciprofloxacin and ceftazidime. Two lineages exhibit the majority of the MDR isolates. A total of 63 different AMR genes are detected, of which 30 are found in 17 different plasmids. Transmissible plasmids such as lncI-gamma/k, IncI1-I(Alpha), Col(pHAD28), IncFIB, IncHI2, and lncI2 that carry AMR genes associated with third-generation antibiotics are also identified. Finally, three new non-synonymous single nucleotide variations (SNVs) for nalidixic acid and eight new SNVs for nitrofurantoin resistance are predicted using genome-wide association studies, comparative genomics, and functional annotation. Our analysis provides for the first time the WGS-based details of the circulating S. Typhimurium lineages and their antimicrobial resistance pattern in Peru.

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Section: Discussionmentioning

confidence: 99%

WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

et al. 2022

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“…The antibiotic multi-resistance presented by Escherichia coli and Salmonella spp. against the antibacterials Gentamicin, Tetracycline, Ampicillin, Penicillin and Novomycin, show that the proven antibiotics possibly underwent modifications of its action mechanism such as: enzymatic inactivation of antibiotics, impermeability of the cell membrane or wall, expulsion by active mechanisms of the antibiotic and modification of the white site of the antibiotic in the bacteria, as reported in the literature, reducing the therapeutic options (Mancera Martínez et al, 2004;Schwarz et al, 2017;Ríos, 2012;Gatica Eguiguren and Rojas, 2018). In addition, the high resistant of 8 antibiotics tested in the study would be linked to the poor application in the health management and the inappropriate use of antibiotics that have been practiced by producers in their herds, and by the irresponsibly massive distribution of drugs by national programs promoted by unskilled professionals, which makes the progressive trend of antibiotic resistance between humans and animals.…”

Section: Discussionmentioning

confidence: 93%

“…and E. coli-Salmonela spp. would be due to causal agents of pathologies that cause intestinal dysfunction, generating the neonatal acute diarrheal in alpacas (More et al, 2011;Mancera Martínez et al, 2004;Ruiz et al, 2014), since Salmonella is an infection belonging to the enterobacteriaceae family (Tacchini A et al, 2010;Ríos, 2012) and there are very few studies of clinical cases of salmonellosis infestation in alpacas, even though this zoonotic pathology is of clinical importance in public health and it is presumed to be present in serovars Typhimurium and Enteritidis by biochemical, macromicroscopic, colonization and clinical manifestations observed in the study.…”

Section: Discussionmentioning

confidence: 99%

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2020

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After a collaborative and uninterrupted work, the UPS has continued to improve one more year as a Research University, and has gone from the position 6,611 in the world and 13 in Ecuador (Webometrics, 2013) to the position 2627 (Webometrics, 2020) worldwide, it is the 170 on the continent and 7 in Ecuador. The continuous improvement of all teams and the strategic vision have made progress in the World Ranking, allowing to go to 3984 positions in 7 years and 366 new positions compared to 2019. Within this global strategy of improvement of the UPS as a Research University, LA GRANJA, belonging to the area of Life Sciences, was the first university publication of Ecuador to be included in SCOPUS, Elsevier publishing house and indexed in the Emerging Source Citation Index of the Web of Science (WOS). This edition presents articles by authors from 5 countries and 16 Universities and Research Centers. We started this number 31 with the topic of weather monitoring, in an effort between the Sciences Department of Environmental Systems of Switzerland and Universidad de Cuenca, led by Dr. Ryan Padrón and his excellent team. In addition, researchers from Universidad de las Fuerzas Armadas, led by Teófilos Toulkeridis, analyze the problem of perceptions in the important and current topic of Climate Change.

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“…Salmonella enterica ha sido reconocida como uno de los patógenos de mayor importancia causante de una enfermedad transmitida por alimentos (ETAs) de carácter zoonótico a nivel mundial. Sin embargo, esta muestra signos y lesiones variables en el humano y en los animales, dependiendo de la patogenicidad, virulencia y resistencia frente a antibióticos de la cepa infectiva (Ríos, 2018;Duran, 2019;Jaja et al, 2019).…”

Section: Introductionunclassified

“…La salmonelosis en cuyes es reconocida como una enfermedad prevalente en el país, mostrándose de forma aguda (mortalidad entre 24-48 horas) o crónica, con signos generales de caquexia, anorexia, debilidad, parálisis de miembros, aborto, ascitis, neumonía y diarrea, entre otros (Morales-Cauti, 2017; Duran, 2019). Sin embargo, la salmonelosis en humanos está relacionada con signos leves, aunque, serovares como S. Typhimurium y S. Enteritidis pueden causar sintomatología gastrointestinal ligada con dolor abdominal, náuseas, vómitos, diarrea y gastroenteritis, hasta septicemia y muerte en los casos más graves (Telles, 2017;Ríos, 2018).…”

Section: Introductionunclassified

Resistencia antibiótica de cepas de Salmonella enterica aisladas de canales de cuyes en un mercado de Lima, 2021

Quispe

Morales-Cauti²,

Dellepiane-Gil³

et al. 2023

Rev. investig. vet. Perú

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El objetivo del presente estudio fue identificar la resistencia antibiótica de cepas aisladas de Salmonella enterica a partir de 384 muestras de canales de cuyes de un mercado de abasto en Lima, Perú. Las muestras fueron inoculadas en caldo de enriquecimiento y sembradas en medios de cultivo específicos. La identificación bacteriana se realizó a partir de pruebas bioquímicas y la resistencia antibiótica mediante el método de difusión en placa frente a 16 antibióticos. Se encontró el 28.9 ± 4.5% (111/384) de muestras positivas a cepas de Salmonella enterica. El 84.7% (94/111) mostró resistencia al menos a uno de los 16 antibióticos, mientras que 15.3% (17/111) fue sensible a todos los antibióticos. Asimismo, 40.5% (45/111) de las cepas mostró multidrogo resistencia (MDR) a tres o más antibióticos. Los resultados confirman el riesgo que representa para la salud pública la comercialización de canales de cuy contaminadas con cepas de Salmonella enterica resistentes a antibióticos.

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Determinación del perfil de resistencia antibiótica de Salmonella enterica aislada de cerdos faenados en un matadero de Lima, Perú

Cited by 5 publications

References 11 publications

WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

WGS-Based Lineage and Antimicrobial Resistance Pattern of Salmonella Typhimurium Isolated during 2000–2017 in Peru

Untitled

Resistencia antibiótica de cepas de Salmonella enterica aisladas de canales de cuyes en un mercado de Lima, 2021

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