Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection

Billerbeck, Eva; Nakamoto, Nobuhiro; Seigel, Bianca; Blum, Hubert E.; Chang, Kyong–Mi; Thimme, Robert

doi:10.1099/vir.0.009837-0

Cited by 11 publications

(7 citation statements)

References 29 publications

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“…In brief, both CD8 low and CD8 high CD57 + subsets are associated with antigen-specific suppressor T cell function. CD8 + CD57 + suppressor cells are generally CD28 - [49,50], and in line with results of this paper, CD8 + CD28lymphocytes have been found to decrease after removal of bladder carcinoma [51].…”

Section: Cd8+ Cd8highcd57+ Cd8highcd57-cd8lowsupporting

confidence: 91%

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

Jacobs¹,

Characiejus²,

Paukonien³

et al. 2010

TOCIJ

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Immunotherapy with BCG is effective in patients with recurrent superficial bladder carcinoma. This therapy involves Interleukin-2 (IL-2), but little is known about the immunological parameters involved in superficial bladder carcinoma. We have monitored immunological parameters in twenty patients with superficial bladder carcinoma treated with transurethral resection (TUR) followed by IL-2 instillation. Cell numbers of peripheral blood leukocyte subpopulations were counted before surgery and during follow-up after surgery. During follow-up, we compared the cell counts in patients with and without a recurrent tumour. We used the values of healthy matched controls as a reference. Recurrent disease in patients corresponded with a significant increase in CD8+ lymphocytes, and especially the CD8highCD57+ and CD8low subpopulations. The phenotype of these T lymphocytes belongs to cells with an immunosuppressive function. We hypothesize that these peripheral immune suppressive cells facilitate tumour recurrences or that tumour recurrences cause an increase in peripheral immune suppressive lymphocytes.

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Section: Cd8+ Cd8highcd57+ Cd8highcd57-cd8lowsupporting

confidence: 91%

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

Jacobs¹,

Characiejus²,

Paukonien³

et al. 2010

TOCIJ

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“…Adaptive CD8 + Tregs have been described in a number of experimental and clinical settings such as following infection with viruses and in response to mycobacteria 15–19. The mechanisms of their actions and induction are varied and include cell killing as well as production of inhibitory factors such as CCL4 19.…”

Section: Discussionmentioning

confidence: 99%

NKG2A is a marker for acquisition of regulatory function by human CD8⁺ T cells activated with anti‐CD3 antibody

et al. 2011

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Summary Treatment with anti-CD3 mAbs modulates immune responses that cause Type 1 diabetes and other diseases. CD8+ Tregs can be induced in vitro and in vivo by the mAb. However, 1/3 of patients do not respond to drug therapy and in an equal proportion, anti-CD3 mAb does not induce Tregs in vitro. The acquisition of CD8+ Treg activity is a function of the CD8+ cells and not the targets in the assay. To identify markers to differentiate responses of CD8+ Tregs, we analyzed genes differentially expressed in CD8+ T cells of non-responders compared to responders, and found that an inhibitory receptor NKG2A (CD159a) was highly expressed in cells from all non-responders tested. An agonistic mAb to NKG2a during in vitro CD8+ Treg induction by anti-CD3 prevented induction of CD8+ Tregs. CD8+ T cells that are TNFR2+ but NKG2A− are the most potent induced Tregs. The level of NKG2A expression on resting CD8+ T cells inversely correlated with acquisition of regulatory function when activated. We suggest that induction of human CD8+ Tregs by anti-CD3 mAb is controlled by a negative signaling through NKG2A, and that NKG2A may serve as a negative marker of human CD8+ Tregs.

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“…NLS skin had increased expression of IL- 4 and Foxp3, which may indicate that NLS skin is primed to become LS skin. In comparison, the cutaneous inflammation in dogs with spontaneous AD was characterised by expression of IL-6, TARC, IL-4 and IL-13 mRNA in the early stages, and IFN-c, IL-12 and IL-18 in the chronic stages and no data are available on Foxp3 expression levels (Olivry et al, 1999;Nuttall et al, 2002;Marsella et al, 2006 (Cosmi et al, 2003;Jarvis et al, 2005;James and Kwok, 2007;Joosten et al, 2007;Mahic et al, 2008;Billerbeck et al, 2009). It would be interesting to investigate whether the CD8 + T cells are responsible for…”

Section: Discussionmentioning

confidence: 99%

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

Veenhof

Knol

Schlotter

et al. 2011

The Veterinary Journal

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a b s t r a c tThe immunopathogenesis of cutaneous adverse food reactions (CAFRs) in dogs is unknown. Since the clinical manifestations in the skin are like those found in canine atopic dermatitis (AD), this study investigated the similarity in T cell phenotypes and gene expression of cytokines and transcription factors in CAFRs. In addition, the influence of an elimination diet on these parameters was tested.In the skin of canine CAFRs, a predominant presence of CD8 + T cells and increased expression of the IL-4, IL-13, Foxp3 and SOCS-3 genes were observed. IFN-c gene expression was increased in lesional compared to non-lesional skin. The predominance of CD8 + T cells indicates that the immunopathogenesis of CAFRs is different from that of canine AD. The elimination diet relieved clinical signs, but did not influence T cell phenotypes or expression of the cytokine and transcription factor genes in the skin of dogs with CAFRs, indicating a continuously pre-activated immune status in dogs sensitised to food constituents.

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Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection

Cited by 11 publications

References 29 publications

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

NKG2A is a marker for acquisition of regulatory function by human CD8⁺ T cells activated with anti‐CD3 antibody

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

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Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection

Cited by 11 publications

References 29 publications

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

Recurrences of Superficial Bladder Carcinoma are Associated with a Raise of CD8high>CD57+ and CD8low T Lymphocytes in Peripheral Blood

NKG2A is a marker for acquisition of regulatory function by human CD8+ T cells activated with anti‐CD3 antibody

Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions

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NKG2A is a marker for acquisition of regulatory function by human CD8⁺ T cells activated with anti‐CD3 antibody