Determination of 16 Selected Trace Elements in Children Plasma from China Economical Developed Rural Areas Using High Resolution Magnetic Sector Inductively Coupled Mass Spectrometry

Abstract

A rapid, accurate, and high performance method of high resolution sector field inductively coupled plasma mass spectrometry (HR-ICP-MS) combined with a small-size sample (0.1 mL) preparation was established. The method was validated and applied for the determination of 16 selected plasma trace elements (Fe, Cu, Zn, Rb, B, Al, Se, Sr, V, Cr, Mn, Co, As, Mo, Cd, and Pb). The linear working ranges were over three intervals, 0-1 μg/L, 0–10 μg/L and 0–100 μg/L. Correlation coefficients (R 2) ranged from 0.9957 to 0.9999 and the limits of quantification (LOQ) ranged from 0.02 μg/L (Rb) to 1.89 μg/L (Se). The trueness (or recovery) spanned from 89.82% (Al) to 119.15% (Se) and precision expressed by the relative standard deviation (RSD %) for intra-day ranging from 1.1% (Zn) to 9.0% (Se), while ranged from 3.7% (Fe) to 12.7% (Al) for interday. A total of 440 plasma samples were collected from Chinese National Nutrition and Health Survey Project 2002 (CNNHS 2002), which represented the status of plasma trace elements for the children aged 3–12 years from China economical developed rural areas. The concentrations of 16 trace elements were summarized and compared by age groups and gender, which can be used as one of the basic components for the formulation of the baseline reference values of trace elements for the children in 2002.

Reference Values of 14 Serum Trace Elements for Pregnant Chinese Women: A Cross-Sectional Study in the China Nutrition and Health Survey 2010–2012

“…The concentrations of 14 serum trace elements, namely iron (Fe), copper (Cu), zinc (Zn), rubidium (Rb), selenium (Se), strontium (Sr), molybdenum (Mo), manganese (Mn), lead (Pb), arsenic (As), chromium (Cr), cobalt (Co), vanadium (V), and cadmium (Cd), were determined by high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS) following 1:20 dilutions of 100 μL of serum with diluent containing 0.5% (v/v) HNO 3 . The detailed analytical method has been reported in previous literature [13]. During the determination, accuracy and precision were checked by certified commercial serum reference materials (Clinchek Level-1, Level-2, Recipe, Germany) and samples of manual serum sample with spiked concentrations of certain elements.…”

Simultaneous analysis of 25 trace elements in micro volume of human serum by inductively coupled plasma mass spectrometry (ICP-MS)

“…The limitation in this study is the use of only Seronorm as a single reference material in contrast to other studies that adopted more than one reference material in assessing the precision and accuracy of their method [31,37]. This shortfall had restricted a fair comparison for several elements that were included in our study due to the difference type of analyser used.…”

“…[32] and Liu et al. [37] in which the use of three to four linear concentration ranges were reported.…”

“…To overcome this masking issue, researchers had suggested sample dilution at a higher fold [58]. Various dilution factors were applied for blood samples in elemental analysis [37,43,59] and this relied on the amount of starting material (sample volume) as well as the number of elements to be included in one single analysis. The challenge surfaced in a situation where sample volume is limited (in microliters) and more elements to be included.…”

Lowered Serum Cesium Levels in Schizophrenia: Association with Immune-Inflammatory Biomarkers and Cognitive Impairments

“…All other reagents used were of AAS grade, and deionized water was used to ensure no leaching of any TEs to the measured standard and samples. Small screw-capped polyethylene containers were used for the preparation of the samples and all analyses were carried out as mentioned previously30 with some modifications18 . Briefly, the deep-frozen serum samples were reconstituted at room temperature and then centrifuged at 2000 g for 15 min.…”