Determination of 2-Amino-1-methyl-6-phenylimidazo[4,5- b]pyridine (PhIP) and Its Metabolite 2-Hydroxyamino-PhIP by Liquid Chromatography/Electrospray Ionization–Ion Trap Mass Spectrometry

Development and validation of a liquid chromatography–tandem mass spectrometry assay for the analysis of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and its metabolite 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP) in plasma, urine, bile, intestinal contents, faeces and eight selected tissues from mice

“…Analyses were performed using a step gradient (Table 1A). Acidifying urine with formic acid to pH 3.5 is of vital importance to guarantee the stability of N-OH-PhIP [27]. A spiked sample of N-OH-PhIP in urine which was not acidified degraded at −20 • C to half its original concentration over a period of 10 months (data not shown).…”

“…A partially validated assay for the analysis of PhIP and N-OHPhIP in human liver was published in 2001 by Prabhu et al [27]. A fully validated bioanalytical liquid chromatography-tandem mass spectrometry method for the quantification of PhIP and its metabolite N-OH-PhIP in plasma, urine, bile, faeces, intestinal contents and eight selected tissues from mice has, to the best of our knowledge, not been published hitherto.…”

Human Cytochrome P450 2s1: Lack of Activity in the Metabolic Activation of Several Cigarette Smoke Carcinogens and in the Metabolism of Nicotine

“…Except for the use of CYP2S1, the methods used for the metabolism study and LC/MS analysis have been well established in our laboratory (Prabhu et al, 2001;Xu et al, 2004;Bao et al, 2005). The incubation mixture (final volume, 200 l for nicotine metabolism and 230 l for PhIP metabolism) consisted of 50 mM Tris-HCl buffer (pH 7.4), 1 mM EDTA, CYP2S1 enzyme (20 pmol), NADPH-P450 reductase (P450/reductase ϭ 10 pmol:30 units), and nicotine (10 or 100 M) or PhIP (200 M).…”

Determination of mutagenic amines in water and food samples by high pressure liquid chromatography with amperometric detection using a multiwall carbon nanotubes-glassy carbon electrode

“…They are also present in cooking fumes (Yen & Hsieh, 1994), several foods (Knize, Kunningham, Griffin, Jones, & Felton, 1994) coffee (Casal, Mendes, Fernandes, Oliveira, & Ferreira, 2004), alcohol beverages (Richling, Decker, Haring, Herderich, & Schraier, 1997), and from environmental sources, such as cigarette smoke (Manabe et al, 1993), air (Manabe et al, 1993), river and rain water (Wu, Wong, Lee, & Ong, 1995). Also, some HAAs have been detected in human tissues (Prabhu et al, 2001), hair (Hegstag et al, 2000), and in biological fluids, such as plasma, urine o bile (Friesen, Garren, Bereziat, Kadlubar, & Lin, 1993), as well as in milk of healthy women (DeBruin, Martos, & Josephy, 2001).…”