Determination of active vitamin B12 (cobalamin) in dietary supplements and ingredients by reversed-phase liquid chromatography: Single-laboratory validation

Vitamin B12 biofortification of soymilk through optimized fermentation with extracellular B12 producing Lactobacillus isolates of human fecal origin

“…The auxotrophic mutant is likely to consume all corrinoids present in tested cell extracts along with the active B12 ( Taranto et al, 2003 ; Chamlagain et al, 2015 ; Bhushan et al, 2016 ). Hence, we used a sophisticated analytical UFLC for characterization (inactive or active-B12) and precise quantification of extracted and purified B12, as suggested by researchers worldwide ( Quesada-Chanto et al, 1998 ; Taranto et al, 2003 ; Chamlagain et al, 2015 , 2017 ; Bhushan et al, 2016 , 2017 ; Edelmann et al, 2016 ; Deptula et al, 2017 ; Bao et al, 2019 ; Qiu et al, 2019 ) and by AOAC First Action Official Method SM 2014.02 ( Giménez and Martin, 2018 ). UFLC quantified the cyanocobalamin on the basis of similar retention times and peak patterns for the standard and the test samples ( Fig.…”

The Importance of Reference Materials and Method Validation for Advancing Research on the Health Effects of Dietary Supplements and Other Natural Products

“…The validated ID-LC-MS/MS method was then employed to certify the content of cyanocobalamin in two multivitamin CRMs recently issued by the National Research Center of Canada (NRCC), i.e., VITA-1 and VITB-1. Qiu et al utilized SRM 3280 in a single-laboratory validation study to demonstrate their HPLC-DAD method for determination of cobalamin in dietary ingredients and DS products (i.e., tablets, capsules, and chewable gels) achieved AOAC International Standard Method Performance Requirements ( 68 ). All of these method validation studies to determine vitamin B 12 in SRM 3280 have provided valuable information toward assessing the true value, as shown in Table 6 .…”

A Fluorescent Probe Based on Polyethyleneimine Protected Copper Nanoclusters for the Assay of Tetracycline Hydrochloride and Vitamin B12

“…[14] Therefore, it is significative to monitor the content of TCH/VB12 in the pharmaceuticals or foods. At present, diverse analytical techniques were employed to monitor TCH/VB12, which included terahertz spectroscopy technology, [2] electrochemical method, [3] SERS, [6] flow injection analysis (FIA) [15] for TCH; liquid chromatography approach, [16] electrochemical method, [17] chemiluminescence, [18] HPLC-DAD method, [19] capillary electrophoresis (CE) [20] for VB12. Nevertheless, these methods demand relatively costly reagents, robust sample handling, low sensitivity or environmentally unfriendly, which will limit their practical applications.…”