Determination of aflatoxins in eggs, milk, meat and meat products using HPLC fluorescent and UV detectors

Herzallah, S. M.

doi:10.1016/j.foodchem.2008.10.077

Cited by 256 publications

(130 citation statements)

References 18 publications

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“…However, there is a lack of modern techniques (LC/MS and HPLC) for studying the presence of AFB1 in body fluids or tissues, and most of the methods were initially limited to their use in food matrices [18,30], although later they were modified for their application to toxicological studies.…”

Section: Resultsmentioning

confidence: 99%

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Validation of a UHPLC-FLD analytical method for the simultaneous quantification of aflatoxin B1 and ochratoxin a in rat plasma, liver and kidney

Corcuera

Ibáñez-Vea

Vettorazzi

et al. 2011

Journal of Chromatography B

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addresses of all coauthors: Laura-Ana Corcuera: lcorcuer@alumni.unav.es, María Ibáñez-Vea: mivea@alumni.unav.es, Ariane Vettorazzi: avettora@alumni.unav.es, Adela López de Cerain: acerain@unav.es. 2 ABSTRACTA rapid and simple method for the simultaneous quantification of AFB1 and OTA in rat plasma, liver and kidney by UHPLC-FLD has been successfully validated according to the following criteria: selectivity, stability, linearity, precision, accuracy, recovery, robustness and limits of quantification and detection. The extraction method, calibration curves and chromatographic conditions are common for the three matrices. Plasma and homogenized tissue samples (100 µL) were extracted with acetonitrile-formic acid mixture (99:1) (300 µL). Chromatographic separation was performed with a mixture of water and acetonitrile:methanol (50:50), both acidified with 0.5% of formic acid using a gradient profile. The method avoids the use of immunoaffinity columns and allows reduction of sample and solvent volumes as well as toxic wastes. The detection is based on a photochemical reaction which enhances the AFB1 response without affecting the OTA signal before reaching the fluorescent detector. The mycotoxin recovery for each matrix was very efficient, between 93% and 96% for AFB1 and between 94% and 96% for OTA. For both mycotoxins the LOQs were 2 µg/L in plasma and 8 µg/kg in liver and kidney. The method has successfully been applied to rat samples after a single oral administration of a mixture of AFB1 and OTA and it could be a useful tool in toxicokinetic and toxicological studies.

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Section: Resultsmentioning

confidence: 99%

“…Reference literature reports numerous methods for individual quantification of AFB1 or OTA, most of which are applied to foodstuffs, but also applied to animal matrices such as poultry [16], fish meat [17] eggs [18], milk [18,19], swine [20], and meat and meat products [18,19].…”

Section: Introductionmentioning

confidence: 99%

Validation of a UHPLC-FLD analytical method for the simultaneous quantification of aflatoxin B1 and ochratoxin a in rat plasma, liver and kidney

Corcuera

Ibáñez-Vea

Vettorazzi

et al. 2011

Journal of Chromatography B

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show abstract

“…HPLC have high efficiency, high sensitivity (HPLC-FLD with as low as 0.1 pg (ng kg -1 ) detecting limit (Herzallah, 2009) and high resolution. And the chromatographic column can be used repeatedly.…”

Section: Hplc Analysis Of Aflatoxinsmentioning

confidence: 99%

Aflatoxin Measurement and Analysis

Hussain¹

2011

Aflatoxins - Detection, Measurement and Control

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“…al., 1994;Ali et. al., 1999;Herzallah, 2009). The accepted limits of AFB1 and total aflatoxins in foods are 5 and 10 µg/kg, respectively, in more than 75 countries around the world whilst they are 2 and 4 µg/kg in the European Union (López et.…”

Section: Aflatoxins In Food Products From Contaminated Animalsmentioning

confidence: 99%