Determination of aflatoxins in food by use of an automatic work station

Niedwetzki, Gunda; Lach, Günter; Geschwill, Klaus

doi:10.1016/0021-9673(94)85188-3

Cited by 23 publications

(7 citation statements)

References 3 publications

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“…These limits certainly do not reach the ng l 21 levels for AFB 1 and AFQ 1 reported in other procedures in the literature (6.8 ng l 21 and 18 ng l 21 , respectively). 12,13 Nevertheless, taking into account the aflatoxins levels reported in human urine (0.5-16 ng ml 21 for AFP 1 , 3 0.1-4.8 ng ml 21 for AFB 1 [19][20][21] ), the method should be suitable. It can be envisaged that with a higher volume of urine sample extracted and with a fluorescence detector of better performance, the limited accuracy of the proposed method will be significantly increased and thus will lie with the best limit of detection reported in the literature.…”

Section: Analytical Figures Of Merit Analysis Of Real Examplesmentioning

confidence: 99%

Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Vázquez¹,

Fente²,

Franco³

et al. 1999

Anal. Commun.

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Section: Analytical Figures Of Merit Analysis Of Real Examplesmentioning

confidence: 99%

Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Vázquez¹,

Fente²,

Franco³

et al. 1999

Anal. Commun.

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“…Aspergilla flavus toxins (Aflatoxin) B 1 , B 2 , G 1 and G 2 were analyzed in the Central Laboratory (Thailand) Company Limited, Chiang Mai Branch, Chiang Mai, Thailand using a multifunctional column (Mycosep) according to the AOAC Official Method 994.08 [31]. In the assay, the ST1-RG drink (250 mL) was extracted with the solvent (acetonitrile: H 2 O = 9:1, v/v) (100 mL) in a blender jar for 1 h and passed through filter paper (cellulose type, Whatman No.…”

Section: Microbiological Testmentioning

confidence: 99%

Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals

et al. 2021

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Rice grass has been reported to contain bioactive compounds that possess antioxidant and free-radical scavenging activities. We aimed to assess rice grass extract (RGE) drink by determining catechin content, free-radical scavenging and iron-binding properties, as well as toxicity in cells and animals. Young rice grass (Sukhothai-1 strain) was dried, extracted with hot water and lyophilized in a vacuum chamber. The resulting extract was reconstituted with deionized water (260 mg/40 mL) and served as Sukhothai-1 rice grass extract drink (ST1-RGE). HPLC results revealed at least eight phenolic compounds, for which the major catechins were catechin, epicatechin and epigallocatechin-3-gallate (EGCG) (2.71–3.57, 0.98–1.85 and 25.47–27.55 mg/40 mL serving, respectively). Elements (As, Cu, Pb, Sn and Zn) and aflatoxin (B1, B2, G1 and G2) contents did not exceed the relevant limits when compared with WHO guideline values. Importantly, ST1-RGE drink exerted radical-scavenging, iron-chelating and anti-lipid peroxidation properties in aqueous and biological environments in a concentration-dependent manner. The drink was not toxic to cells and animals. Thus, Sukhothai-1 rice grass product is an edible drink that is rich in catechins, particularly EGCG, and exhibited antioxidant, free radical scavenging and iron-binding/chelating properties. The product represents a functional drink that is capable of alleviating conditions of oxidative stress and iron overload.

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“…Chromatographic methods (4–7) using liquid chromatography (LC) in conjunction with fluorescence detection (FLD) (8) or mass spectrometry detection (MS) (9) are the most commonly employed to measure AfB1 at the concentrations present in food commodities. Niedwetzki and Geschwill (10) developed an LC‐based automatic workstation for determining aflatoxins in food.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Aspergillus flavus Contamination in Peanut with Novel Delayed Luminescence Spectra

Chen

Xing

Chen

2005

Photochem & Photobiology

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Employing a luminescence spectrometer the usefulness of light-induced delayed luminescence (DL) for the detection of aflatoxinB1 (AfB1) contamination in whole peanut was studied. Peanut was artificially contaminated with Aspergillus flavus (7.5 3 10 5 conidia/mL) and incubated for 0-72 h. The DL and fluorescence spectra of contaminated peanut were obtained by a luminescence spectrometer. The correlation between the spectra and contamination levels was established. The DL and fluorescence intensity has a negative correlation with the AfB1 concentration. Our results suggested that the DL technique might be useful for the rapid and noninvasive evaluation of AfB1 contamination levels in whole peanut.

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Determination of aflatoxins in food by use of an automatic work station

Cited by 23 publications

References 3 publications

Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Identifying Chemical Composition, Safety and Bioactivity of Thai Rice Grass Extract Drink in Cells and Animals

Rapid Detection of Aspergillus flavus Contamination in Peanut with Novel Delayed Luminescence Spectra

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