Determination of aminoglycoside residues by liquid chromatography and tandem mass spectrometry in a variety of matrices

Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

“…The results showed that for apramycin, gentamicin and neomycin the sensitivity acquired (abundance of precursor ion) was rather insufficient, bearing in mind the low MRL of gentamicin in muscle (50 g kg −1 ). These compounds are the most strongly retained AGs in HILIC columns and they often need a very significant increase of the aqueous phase content in the mobile phase to be eluted (up to 95% aqueous phase) [39,55].…”

“…composes a great challenge in multiresidue analysis and has rarely been reported previously. An additional difficulty is presented in obtaining a final extract suitable for AGs' determination and HILIC-compatible (>60% ACN) at the same time, since AGs are not efficiently ionized at low water content solvents [55].…”

An integrated electrophoretic mobility control device with split design for signal improvement in liquid chromatography–electrospray ionization mass spectrometry analysis of aminoglycosides using a heptafluorobutyric acid containing mobile phase

“…To make the mobile phase more compatible with ESI-MS, separation based on hydrophilic interaction chromatography (HILIC) has been reported [18e21]. However, the performance may not be better than the use of HFBA, especially for late eluting aminoglycosides [12].…”

Synthesis of molecularly imprinted polymer sorbents and application for the determination of aminoglycosides antibiotics in honey

“…Owing to their highly hydrophilic property, AAs are always poorly retained on the common C18 column or sorbent and thus it is not easy to achieve their effective separation via common C18 column. To address the issue, the separation modes based on ultra-performance liquid chromatography (UPLC) [3], ion pair chromatography (IPC) [2] and hydrophilic interaction chromatography (HILIC) [4] have been used for separation of AAs recently.…”

“…Among many methods, solid phase extraction (SPE) is the most widely used one owing to its some advantages such as easy operation, simplicity and low cost. C18 and weak exchange resin-based SPE have been reported for pretreating AA-contained food samples [2,3]. Their common drawback lies in limited selectivity, for example, 72.5%, 27.4% and 64.9% of the recoveries for streptomycin, streptomycin and dihydrostreptomycin were obtained by the use of C18 resinbased SPE cartridge [3], while for weak exchange resin-based SPE, 51-76% of recoveries for 11 AAs was obtained by Babin and Fortier [5] and 85.5% and 72.3% of recoveries for streptomycin and dihydrostreptomycin was reported by Gremilogianni et al [2].…”