Determination of antibacterial activity, total phenolic, flavonoid and saponin contents in leaves of Anogeissus leiocarpus (DC.) Guill and Perr

Edewor, Theresa Ibibia; Akpor, Oghenerobor B.; Owa, S. O.

doi:10.12980/jclm.4.2016j5-218

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…leiocarpus activity against E. coli (12 mm to 16 mm) and (9 mm to 14 mm) for S. aureus respectively. Similarly, leaf extract have been reported to be active against P. aeruginosa and K. pneumonia [23],…”

Section: Discussionmentioning

confidence: 96%

Anogeissus leiocarpus: A Potential Inhibitor of Some ExtendedSpectrumβ-Lactamase Bacteria

Dahiru¹,

Adamu²,

Kolawole³

et al. 2021

Act Scie Micro

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Section: Discussionmentioning

confidence: 96%

Anogeissus leiocarpus: A Potential Inhibitor of Some ExtendedSpectrumβ-Lactamase Bacteria

Dahiru¹,

Adamu²,

Kolawole³

et al. 2021

Act Scie Micro

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“…This antioxidant mechanism will usually prolong the average life span of individual RBCs. A. leiocarpus have been reported to contain high flavonoid content (31,33,56) and flavonoids in A. leiocarpus have been identified as responsible for the scavenging or chelating activity against oxidative stress (26,57). Different researches have shown that the expression of EPO can be modulated by flavonoids.…”

Section: Discussionmentioning

confidence: 99%

“…The plant has been reported to possess antiproliferative properties against HepG2 hepatocarcinoma cells (29), antitumor activity via angiogenesis pathway (30), as well as antioxidant and antimicrobial activities (30)(31)(32). Phytochemical screening identified the presence of flavonoids, terpenoids, tannins, alkaloids, cardiac glycosides, saponins, steroids, anthraquinones, and phenolic compounds in the plant (26,33).…”

mentioning

confidence: 99%

Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals

Elufioye

Williams

Cyrl-Olutayo

2020

Avicenna J Med Biochem

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Background: The anti-sickling activity of Anogeissus leiocarpus, a plant used for managing sickle cell disease (SCD), has been previously proven. Objectives: This study investigated the anti-sickling mechanism of A. leiocarpus by probing its effects on Gardos channel (KCNN4), erythropoietin (EPO), erythropoietin receptor (EPOR), catalase (CAT), G6pD, D-type cyclins and cyclin-dependent kinase inhibitors (p21) gene expression as well as assessing in silico drug-likeness of reported compounds as EPOR agonist. Methods: A total of 18 rats (45-76 g) were selected and divided into 6 groups (n=3). The control group was given water ad libitum, standard group was given 0.1 mL/kg of Ciklavit® and experimental group was given daily oral doses of 50-100 mg/kg body weight of crude methanol extract or ethyl acetate fraction (EA-PF). Haematological parameters were analyzed while histopathological and molecular studies of kidney and bone marrow were carried out, followed by RT-PCR analysis of KCNN4, EPO, EPOR, CAT, G6pD, p21, and cyclin-dependent kinase inhibitors. Docking studies of the reported compounds were also done. Results: EA-PF had an insignificant (P>0.05) effect on haematological parameters compared to the basal group. While CAT and p21 acted in a positive feedback loop, G6pD was downregulated in the experimental groups. KCNN4 acted in a negative-feedback mechanism and the upregulation of EPO and EPOR was followed by increased reticulocytes. Kaempferol, quercetin, and catechin showed non-violation of Lipinski’s rule and high binding affinities of 6.5 kcal/mol, 6.7 kcal/mol, and 6.7 kcal/mol, respectively, for EPOR pocket compared to the co-crystallized ligand. Conclusion: Results suggest that ethyl acetate fraction of Anogeissus leiocarpus achieved a steady state level of the Gardos channel and stimulation of EPO expression via EPOR agonist.

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“…This is not astonishing because it is expected that standard antibiotics should exert superior activity due to their refined natured compared to crude extracts. The relatively thin peptidoglycan layer of gram-negative bacteria and an outer phospholipidic membrane contain lipopolysaccharide components that result in lipophilic solute impermeability for Gram-positive bacteria, outer peptidoglycan layers are thick thus, not an effective and excellent permeable barrier which increase susceptibility to the plant extract (57). The MIC spanned from 6.25 to 12.5 mg/mL which is lower than the values previously documented (54,58), attributed to the presence of active phytochemical constituents which inhibits bacterial growths.…”

Section: Sciences Of Pharmacymentioning

confidence: 99%

Phytochemical Analysis, In-vitro, and In-silico Antibacterial Activity of Stembark Extract of Anogeissus leiocarpus (DC) Guill and Perr

Dahiru,

Abaka,

Musa

2023

sciphar

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Bacterial infections subsequently leading to antibiotic resistance has been a leading cause of mortality and morbidity worldwide especially in developing countries with high poverty rate and poor healthcare system. Thus, prompting the prospect in alternative therapy such as medicinal plants. In the present study, we evaluated the antibacterial action of stem bark extract of Anogeissus leiocarpus (AL) Guill and Perr. as applied in folkloric medicine for antibacterial purposes. The phytochemicals present in the plant extract were identified and quantified, followed by the determination of the antibacterial effects of the extract against Escherichia coli and Staphylococcus aureus. Molecular docking study was carried out to ascertain the inhibitory effects of compounds from AL against bacterial enzymes. Alkaloids (7.17% ±0.60), saponins (11.33% ±3.18), and flavonoids (31.01% ±4.04) were detected. A maximum ZI was observed for E. coli compared to S. aureus at the highest extract concentration (100 mg/mL) with amoxicillin having superior ZI at 50 mg/mL concentration. The MIC against E. coli and S. aureus were 12.5 mg/mL and ≤ 6.25 mg/mL respectively while the MBC was>100 mg/mL and 100 mg/mL respectively. Among the identified compounds, IX exhibited the least binding affinity (BA) (7.2 kcal/mol) and inhibition constant (Ki) (5 µM) against UDP-N-acetylglucosamine Enolpyruvyl Transferase (Mur A) compared to all the other targets. AL demonstrated antibacterial activity evidenced by the bacterial growth inhibition, bactericidal potential, and in-silico study revealing high affinity of the bacterial enzymes for the identified compounds, thereby supporting the acclaimed antibacterial use of the plant in folkloric medicine.

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Determination of antibacterial activity, total phenolic, flavonoid and saponin contents in leaves of Anogeissus leiocarpus (DC.) Guill and Perr

Cited by 6 publications

References 12 publications

Anogeissus leiocarpus: A Potential Inhibitor of Some ExtendedSpectrumβ-Lactamase Bacteria

Anogeissus leiocarpus: A Potential Inhibitor of Some ExtendedSpectrumβ-Lactamase Bacteria

Identification of the Anti-sickling Activity of Anogeissus leiocarpus and In Silico Investigation of Some of Its Phytochemicals

Phytochemical Analysis, In-vitro, and In-silico Antibacterial Activity of Stembark Extract of Anogeissus leiocarpus (DC) Guill and Perr

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