Determination of Ascorbic Acid (Vit. C) in the Presence of Dehydroascorbic Acid Using PMR Spectroscopy

Al-Ashmawi, Mohamed; Metwally, Mamdouh; Saleh, Hanaa; Mansour, O.

doi:10.1080/00032719208020059

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The use of dimethyl sulfoxide (DMSO) can be referenced from the literature as a solvent for dissolving the matrices of fat soluble and water soluble vitamin concentrates (Al-Ashmawi et al, 1992;Dalbacke and Dahlquist, 1991;Qureshi et al, 1990;Beaulieu et al, 1989;Indyk, 1988). Based upon these references and some in-house development work, an alternate sample preparation method has been optimized using 10% (v/ v) aqueous DMSO.…”

Section: Introductionmentioning

confidence: 99%

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃

Pastore¹,

Dunnett²,

Webster³

1997

J. Agric. Food Chem.

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A more time efficient and simpler method than methods based upon AOAC procedure 979.24 was developed for the analysis of concentrated microencapsulated vitamin D3. The improved efficiency and simplicity begins with the use of a 10% water in DMSO solution to dissolve the sample matrix. A 2.0 mL DMSO volume is combined with 200 mg of microencapsulated vitamin D3 in a flask or bottle. The mixture stands without agitation for 10 min when testing a starch matrix sample and for 30−60 min when testing a cellulose matrix sample. Five minutes of sonication or heating is required for vitamin A/D combinations. Next, 2,2,4-trimethylpentane (isooctane) is pipetted into the DMSO solution−sample mixture. The isooctane volume used was proportional to the sample concentration; specific isooctane volumes relative to sample concentrations are provided. The mixture is stirred for ca. 5 min using a magnetic stirrer. The supernatant is filtered through a 0.2 μm PTFE membrane into a suitable LC vial and chromatographed. Validation data which support equivalence to traditional AOAC 979.24-based methodology are presented. Keywords: Vitamin D; DMSO extraction; vitamin analysis

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Section: Introductionmentioning

confidence: 99%

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃

Pastore¹,

Dunnett²,

Webster³

1997

J. Agric. Food Chem.

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Non-spectrophotometric methods for the determination of Vitamin C

Arya

Mahajan

Jain

2000

Analytica Chimica Acta

197

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A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using Iron(III)-Ferronate Complex

Arya

Mahajan

1994

ANAL. SCI.

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Determination of Ascorbic Acid (Vit. C) in the Presence of Dehydroascorbic Acid Using PMR Spectroscopy

Cited by 3 publications

References 6 publications

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃

Non-spectrophotometric methods for the determination of Vitamin C

A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using Iron(III)-Ferronate Complex

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Determination of Ascorbic Acid (Vit. C) in the Presence of Dehydroascorbic Acid Using PMR Spectroscopy

Cited by 3 publications

References 6 publications

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D3

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D3

Non-spectrophotometric methods for the determination of Vitamin C

A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using Iron(III)-Ferronate Complex

Contact Info

Product

Resources

About

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃

Dimethyl Sulfoxide Extraction Method for the Liquid Chromatographic Analysis of Microencapsulated Vitamin D₃