Determination of avian influenza A (H9N2) virions by inductively coupled plasma mass spectrometry based magnetic immunoassay with gold nanoparticles labeling

Xiao, Guangyang; Chen, Beibei; He, Man; Shi, Kaiwen; Xing, Zhang; Li, Xiaoting; Wu, Qiumei; Pang, Dai‐Wen; Hu, Bin

doi:10.1016/j.sab.2017.10.015

Cited by 22 publications

(16 citation statements)

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“…Within this context, single chelates [4], commercial polymers containing several lanthanide atoms covalently attached [5], metal nanoparticles (NPs) most commonly made of gold [6,7] with diameters in the order of 20-50 nm, and quantum dots (QDs) [8,9] have been successfully investigated as elemental labels. The use of labels with many atoms of a given metal per tag will allow high signal amplification, and thus, good sensitivity for detection of the corresponding biomolecule.…”

Section: Introductionmentioning

confidence: 99%

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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The authors describe the use of platinum nanoclusters (PtNCs) as bimodal labels in a competitive immunoassay for immunoglobulin E (IgE). Both fluorometry and inductively coupled plasmamass spectrometry (ICP-MS) are used. Optimization of the PtNCs synthesis process using lipoic acid as ligand was carried out. The time for synthesis and the effect of NaOH added to the PtNCs precursor mixture was optimized with the aim to obtain PtNCs with strong fluorescence and low size dispersity. Maximal fluorescence was obtained at excitation/emission wavelengths of 455/620 nm. The average diameter (1.5 nm) and crystal structure (face-centered cubic structure) of the PtNCs were determined by HR-TEM. It was calculated that each PtNC contains 116 Pt atoms at average. Labelling of the antibody (Ab) against IgE with PtNCs was optimized in terms of recognition capabilities and fluorescence signal. A molar ratio (Ab:PtNCs) of 1:11 is found to be best. A competitive immunoassay for IgE was developed and detection was carried out by using both ICP-MS (by measuring 195 Pt) and fluorometry. The limit of detection (LOD) of the fluoroimmunoassay is 0.6 ng mL-1 of IgE. The LOD of the ICP-MS method is as low as 0.08 ng mL-1. The method was evaluated by analyzing four (spiked) serum samples by ICP-MS. No sample pretreatment excepting dilution is needed. Results compared favorably with those obtained by a commercial ELISA kit.

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Section: Introductionmentioning

confidence: 99%

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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“…A large variety of NPs have been proposed (AgNPs [52][53][54][55], PtNPs [53,56], PbSNPs [57], metal/dye-doped silica NPs [58], Cs-doped magnetic NPs [58,59], UCNPs [30,31], EuPO 4 /LuPO 4 NPs [32], Quantum Dots (QDs) [60][61][62][63][64][65], etc.) but AuNPs [34,[53][54][55]63,[66][67][68][69][70][71][72][73][74][75][76] are usually preferred due to their lower cost and greater commercial availability. When compared to REE-based chelates, the use of inorganic NPs affords some attractive and unique features.…”

Section: Rare Earth Elements and Transition Metal-based Npsmentioning

confidence: 99%

“…In general, NPs have been mainly employed for single analyte determinations [31,54,56,57,59,60,62,[64][65][66][67][69][70][71][74][75][76]. Unlike REEbased chelates, and despite the vast number of described NPs proposed in the literature for immunoassays, the use of NPs for multiplexed applications has been scarcely exploited [32,53,55,58,63].…”

Section: Rare Earth Elements and Transition Metal-based Npsmentioning

confidence: 99%

“…cytokeratin fragment antigen 21-1, carcinoembryonic antigen (CEA) and carbohydrate antigen (CA15-3)) operating a competitive sandwich immunoassay with antibody-coated magnetic beads and detection antibodies conjugated with AuNPs, AgNPs and ZnSeQDs. From our point of view, the lack of multiplexed applications could be attributed to different factors compromising practical analysis such as: (i) NPs limited stability and aggregation phenomena [78]; (ii) signal-tobackground deterioration by non-specific adsorption [34,60,61]; (iii) limited precision due to NP size heterogeneity [63,70]; and (iv) the absence of robust protocols for a reproducible conjugation of antibodies with NPs of different composition/characteristics. Nevertheless, recent works have demonstrated that the above-mentioned shortcomings can be mitigated.…”

Section: Rare Earth Elements and Transition Metal-based Npsmentioning

confidence: 99%

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Immunoassays based on inductively coupled plasma mass spectrometry detection: So far so good, so what?

Torregrosa

Grindlay

Gras

et al. 2021

Microchemical Journal

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“…Inductively coupled plasma mass spectrometry (ICP-MS) as an element-specific detector has high sensitivity, wide linear range, strong anti-matrix interference ability, high sample throughput and multi-element analysis ability. ICP-MS based on element labeling for bioanalysis has become a powerful biological quantitative analysis tool in recent years, and has been widely used in the analysis of proteins [ [7] , [8] , [9] ], nucleic acids [ 10 , 11 ], viruses [ 12 ], cells [ 13 , 14 ], and so on. For instance, Tanner’s group had simultaneously detected multiple proteins in complex samples by using the element-tagged antibodies [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

Chen

et al. 2021

Analytica Chimica Acta

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In recent years, single particle inductively coupled plasma mass spectrometry (SP-ICP-MS) has become a powerful tool for biological quantitative analysis. Homogeneous analysis method requires no separation and washing steps, which is suited for the analysis of highly infectious pathogens, so as to reduce the risk of infection during the operation. SARS-CoV-2 spreads all over the world, and its early infection symptoms are similar to influenza, which brings inconvenience to triage. Therefore, developing novel analytical method for simultaneous detection of multiple viral nucleic acids is essential. Taking the advantages of SP-ICP-MS and homogeneous analysis strategy, a SP-ICP-MS homogeneous nucleic acid assay by using gold nanoparticles (Au NPs) and silver nanoparticles (Ag NPs) probes was established for simultaneous sensitive analysis of SARS-CoV-2 and influenza A (H3N2). In the present of target SARS-CoV-2 or H3N2 nucleic acids, corresponding Au NPs or Ag NPs probes form larger aggregates, resulting in increased pulse signal intensity and reduced pulse signal frequency of the corresponding NPs in SP-ICP-MS measurement. In this assay, the reaction system of Au NPs and Ag NPs probes does not interfere with each other, and there was no separation and washing procedure, which facilitates operation, saves the analysis time, and improves the analysis efficiency. The linear range of this method is 5-1000 pmol L -1 , with low-level limits of quantification of target nucleic acid. The developed SP-ICP-MS simultaneous homogeneous detection method has a good potential for detecting nucleic acid, protein, cell and other biological samples by changing different modification sequences on the NPs probes.

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Determination of avian influenza A (H9N2) virions by inductively coupled plasma mass spectrometry based magnetic immunoassay with gold nanoparticles labeling

Cited by 22 publications

References 50 publications

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Immunoassays based on inductively coupled plasma mass spectrometry detection: So far so good, so what?

A homogeneous nucleic acid assay for simultaneous detection of SARS-CoV-2 and influenza A (H3N2) by single-particle inductively coupled plasma mass spectrometry

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