Determination of Beta-Blockers in Biological Material

Marko, Vladimir

doi:10.1097/00007691-199005000-00020

Cited by 4 publications

(6 citation statements)

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“…This electrochemical response for the ATN at pH values below 7.0 is blocked by the acid-base equilibrium of the amino group whose pKa = 9.4, 21 as represented in Eq. (1). …”

Section: Resultsmentioning

confidence: 99%

“…β-blockers are exceptionally toxic and most have a narrow therapeutic range; i.e., the differences between the lowest therapeutic and the highest tolerable doses are small. [1][2][3] Hence, accurate methods for the measurement of ATN are of great importance in pharmaceutical research. Several methods have been reported for the determination of ATN, including UV spectrophotometry, 4,5 spectroflurometry 6 and capillary zone electrophoresis in plasma.…”

Section: Introductionmentioning

confidence: 99%

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Nanogold-modified Carbon Paste Electrode for the Determination of Atenolol in Pharmaceutical Formulations and Urine by Voltammetric Methods

Behpour¹,

Honarmand²,

Ghoreishi³

2010

Bulletin of the Korean Chemical Society

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A gold nanoparticles modified carbon paste electrode (GN-CPE) has been used for the determination of atenolol (ATN) in drug formulations by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronocoulometric methods. The results revealed that the modified electrode shows an electrocatalytic activity toward the anodic oxidation of atenolol by a marked enhancement in the current response in buffered solution at pH 10.0. The anodic peak potential shifts by -80.0 mV when compared with the potential using bare carbon paste electrde. A linear analytical curve was observed in the range of 1.96 × 10 -6 to 9.09 × 10 -4 mol L -1 . The detection limit for this method is 7.3 × 10 -8 mol L -1 . The method was then successfully applied to the determination of atenolol in tablets and human urine. The percent recoveries in urine ranged from 92.0 to 110.0%.

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“…This electrochemical response for the ATN at pH values below 7.0 is blocked by the acid-base equilibrium of the amino group whose pKa = 9.4, 21 as represented in Eq. (1). …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanogold-modified Carbon Paste Electrode for the Determination of Atenolol in Pharmaceutical Formulations and Urine by Voltammetric Methods

Behpour¹,

Honarmand²,

Ghoreishi³

2010

Bulletin of the Korean Chemical Society

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“…The ester group present in the structure of esmolol undergoes rapid hydrolysis in blood, leading to quick onset of the pharmacological effect as well as its rapid abatement. Its metabolite, 3-{4-[2-hydroxy-3-(propan-2-ylamino)propoxy]phenyl}propionic acid, has 400 times lower activity toward β 1 -receptors than esmolol [123].…”

Section: Metabolismmentioning

confidence: 99%

Survey of Pharmacological Activity and Pharmacokinetics of Selected β-Adrenergic Blockers in Regard to Their Stereochemistry

et al. 2019

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The present survey concentrates on pharmacodynamics and pharmacokinetics of selected β-adrenergic blockers from the point of view of their stereochemistry. It could be shown that the activity in the arylaminoethanol and aryloxyaminopropanol group of β-blockers is higher in their (–)-enantiomers as compared with the (+)-enantiomers. The stereoisomers differ also in other types of bioactivity as well as in toxicity. The particular pharmacokinetic stages such as resorption, distribution, and metabolism are discussed in regard to their stereochemistry.

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“…Labetalol hydrochloride, 2-hydroxy-5-[1-hydroxy-2-(1-methyl -3 phenyl propylamino) ethyl] benzamide hydrochloride, is an adrenergic β-receptor blocking agent used in the treatment of hypertension, which exhibits both α-and β-adrenoceptor blocking activity 1,2 , and because of its use as doping agent in sports, this drug has been added to the list of forbidden substances issued by the International Olympic Committee. Therefore, the development of an analytical method sensitive and selective enough for determining Labetalol in both pharmaceutical and biological samples is of great importance.…”

Section: Introductionmentioning

confidence: 99%

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2010

IJPSR

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A simple and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for estimation of Labetalol in human plasma, an adrenergic β-receptor blocking agent used in the treatment of hypertension, which exhibits both α-and β-adrenoreceptor blocking activity. The analyte and internal standard (Metoprolol) were extracted by liquid/liquid extraction with ethyl acetate. The chromatographic separation was performed on reverse phase Phenomenax Luna column (C18, 5µm, 100 x 4.6 mm) with a mobile phase of 2 mM ammonium formate (pH 5.0) / methanol (20:80 v/v), which was pumped at a flow rate of 0.5 mL / min with split ratio of 20:80. The protonated analyte was quantitated in positive ionization by multiple reactions monitoring with a mass spectrometer. The mass transitions m/z 329.01→161.95 and 267.99→115.86 were used to measure Labetalol and Metoprolol, respectively. The lower limit of quantitation was 3.1800 ng/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the calibration curve ranges (3.1800 to 700.8760 ng/ml). Sample analysis time of 2.5 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day.

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Determination of Beta-Blockers in Biological Material

Cited by 4 publications

References 0 publications

Nanogold-modified Carbon Paste Electrode for the Determination of Atenolol in Pharmaceutical Formulations and Urine by Voltammetric Methods

Nanogold-modified Carbon Paste Electrode for the Determination of Atenolol in Pharmaceutical Formulations and Urine by Voltammetric Methods

Survey of Pharmacological Activity and Pharmacokinetics of Selected β-Adrenergic Blockers in Regard to Their Stereochemistry

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