Determination of beta-haemolytic activity and minimum inhibitory concentrations of antimicrobial drugs against Aeromonas hydrophila strains isolated from fish products

Stratev, Deyan; Vashin, I.; Daskalov, H.

doi:10.15547/bjvm.836

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…It is responsible for "red leg diseases" and is involved in cases of mass mortality of frogs in captivity and in the natural environment (Forbes et al, 2004). This presence in frogs may also constitute a public health risk for consumers because A. hydrophila is an emerging pathogen involved in gastroenteritis, septicemia and endocarditis (Stratev et al, 2012). The areas where frogs are caught are generally shallows, abandoned fish ponds of questionable microbiological quality.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

2024

Int. J. Biosci.

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Aeromonas hydrophila is an opportunistic microorganism. It is widely involved in frog infections.Hoplobatrachus occipitalis is the species of frog most consumed in the different localities of Côte d'Ivoire. This research aimed to study the extracellular virulence factors and antibiotic resistance profiles of A. hydrophila strains isolated from H. occipitalis. The determination of extracellular virulence factors were performed onto 113 strains of A. hydrophila isolated from frogs H. occipitalis using conventional methods. The antimicrobial susceptibility tests were performed by the disk diffusion method using 20 isolates of A. hydrophila. All strains isolated produce nuclease (100%) but the ability to produce haemolysins, lipases, amylases and proteases varied from one isolate to another These microorganisms were the most resistant to Amoxicillin (95%), Ampicillin (90%), Tetracyclin (85%), Ceftriaxone (80%) Chloramphenicol (75%). All strains of A. hydrophila have developed multi-resistance to different antibiotics used. The multiple antibiotic resistance indices ranged from 0.41 to 0.83. These data show the degree of pathogenicity of the strains isolated from frogs. The consumption of this species could constitute a threat to consume them if hygiene measures are not taken into account.

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Section: Introductionmentioning

confidence: 99%

Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

2024

Int. J. Biosci.

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“…Aeromonas hydrophila is a widely existing motile [1], aerobic, Gram negative, mesophilic bacteria, positive for oxidase-and catalase [2], belonging to Aeromonadaceae family [3]. These bacteria can grow and produce toxins at a wide range of temperatures °C [4] indicating that storing food at refrigeration seems to be ineffective to control these pathogens [5] and due to their capability to grow in refrigerated food [6] they pose a serious risk to people safety and health [7,8]. Aeromonas spp.…”

Section: Introductionmentioning

confidence: 99%

Association Between Antimicrobial Resistance and Biofilm Production of Aeromonas hydrophila Isolated from Beef and Mutton in Duhok Abattoir

Yousif

Jakhsi

taher

2023

Egyptian Journal of Veterinary Sciences

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T HE study aimed to investigate the presence of A. hydrophila in mutton and beef, and to determine the possible association between resistance to antibiotics and biofilm formation ability. A total of 91 meat samples; (60) mutton and (31) beef collected from local slaughterhouse in Duhok city. All samples were cultured and A. hydrophila strains were isolated using microbiological and biochemical assays then confirmed molecularly by amplifying 16S rRNA gene. The antimicrobials susceptibility test was implemented using Kirby-Bauer procedure and biofilm formation ability was quantified by micro-titer plate appliance. The bacterial identification revealed that 41(45.05%) isolates were belonging to A. hydrophila distributed as 28/60 (46.67%) for mutton and 13/31 (41.93%) for beef. Antibiotic susceptibility test results showed that the highest resistance was recorded for Cephalothin, Amoxicillin, and Tetracycline at a rate of 90.24%, 82.92%, and 85.36% respectively, while high sensitivity to Nitrofurantoin, Chloramphenicol, Cefixime, Trimethoprim, Ciprofloxacin, and Ceftriaxone was detected in 95.12%, 92.68%, 97.56%, 90.24%, 90.24%, and 97.56% of the isolates, respectively. The biofilm formation assay demonstrated that (90.23%) of obtained isolates were biofilm producers distributed among strong, intermediate and weak and there was a positive association between antibiotic resistance and biofilm formation. Our study's findings imply that the occurrence of A. hydrophila in food meats is a significant health hazard and may cause foodborne illnesses. Because Aeromonads can endure cold temperatures, and thrive in a variety of habitats, it is important to pay them more attention and strict sanitary practices should be used to limit bacterial contamination.

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“…Some virulence genes were found in the isolated A. hydrophila, such as cytotoxic enterotoxins heat-stable (ast) and cytotoxic enterotoxin (act), while cytotoxic enterotoxin (alt) and hemolysin (hly) genes were absent. In contrast, the hly gene was present in A. hydrophila strains, and by amplification, the PCR products of 1500 bp were given [12,13]. Aerolysin and cytotoxic enterotoxin are an important and reliable molecular indicator for finding potentially pathogenicity of Aeromonas species .…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of 16S rRNA and Some Virulence Genes From Aeromonas spp. in Nasser Lake Fish Species, Aswan, Egypt

Mokhtar

2023

Egyptian Journal of Veterinary Sciences

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F RESHWATER fish consider a major cheap source of animal protein in different parts of the world. In upper Egypt, Lake Nasser is the main source of fish production for human consumption. Fish can also be a cause of foodborne pathogens, such as the Aeromonas species, that pose a significant risk to public health. A total of 180 freshwater fish samples were represented, Nile tilapia, Nile perch, Pike perch, African sharp tooth catfish, Elephant-snout, and Fillet tilapia (30 of each) were randomly selected from Lake Nasser in Aswan province. Bacteriological examination reveals 117 (65%) occurrence of Aeromonas species. In isolated 60 Aeromonas species samples10 from each fish species, 16S rRNA genes were found with a percentage of 96.67% by using PCR. In Egypt and around the globe, the Aeromonas species has been linked to several foodborne outbreaks brought on by the consumption of raw fermented fish which having many sever virulence gene . Additionally, two genes; Aerolysin (aer) and cytotoxic enterotoxin (act) genes were selected to be screened by PCR. Results were 51.7% and 27.6%, respectively. So hygienic precautions must be taken to eradicate Nasser Lake Fish species contaminated with Aeromonas species. Molecular investigating of some virulence genes of Aeromonas spp. in Lake Nasser from different fish species was the aim of the present study.

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Determination of beta-haemolytic activity and minimum inhibitory concentrations of antimicrobial drugs against Aeromonas hydrophila strains isolated from fish products

Cited by 4 publications

References 28 publications

Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

Antibiotic resistance of Aeromonas hydrophila isolated from frog Hoplobatrachus occipitalis consumed in Côte d’Ivoire

Association Between Antimicrobial Resistance and Biofilm Production of Aeromonas hydrophila Isolated from Beef and Mutton in Duhok Abattoir

Molecular Identification of 16S rRNA and Some Virulence Genes From Aeromonas spp. in Nasser Lake Fish Species, Aswan, Egypt

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