Determination of bioavailable-zinc from biofortified wheat using a coupled in vitro digestion/Caco-2 reporter-gene based assay

Salunke, Rajani; Rawat, Nidhi; Tiwari, Vijay K.; Neelam, Kumari; Randhawa, G. S.; Dhaliwal, H. S.; Roy, Partha

doi:10.1016/j.jfca.2011.09.006

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…Unmilled rather than white (milled) kernels were evaluated to allow detection of variation in concentrations of elements that primarily accumulate in bran as well as those that accumulate primarily in endosperm and to prevent confounding the data with variance from potential milling differences Saenchai et al, 2012). The study of whole-grain brown rice makes this study consistent with, and more directly relevant to, biofortification research in other grain crops such as wheat (Triticum aestivum L.) (Salunke et al, 2012;Wu et al, 2013;Borrill et al, 2014), maize (Zea mays L.) (Baxter et al, 2012a), and pearl millet (Pennisetum glaucum) (Cercamondi et al, 2013) as well as prior studies in Arabidopsis thaliana (Vreugdenhil et al, 2004;Baxter et al, 2012b) and rice (e.g., Norton et al, 2012Norton et al, , 2014Kuramata et al, 2013;Zhang et al, 2014). The convention in this report will be to report mineral concentrations as micrograms per gram grain dry weight (g g -1 ) and to present the five plant macronutrients first (P, Mg, K, S, Ca) in high to low order of their grain concentration, followed by the remaining 11 elements in alphabetical order (As, Cd, Co, Cu, Fe, Mn, Mo, Ni, Rb, Sr, Zn).…”

Section: Determination Of Element Concentrations In Unmilled Rice Grainmentioning

confidence: 96%

Worldwide Genetic Diversity for Mineral Element Concentrations in Rice Grain

et al. 2015

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With the aim of identifying rice (Oryza spp.) germplasm having enhanced grain nutritional value, the mineral nutrient and trace element concentrations (or ionome) of whole (unmilled) grains from a set of 1763 rice accessions of diverse geographic and genetic origin were evaluated. Seed for analysis of p, Mg, K, S, Ca, As, Cd, Co, Cu, Fe, Mn, Mo, Ni, rb, Sr, and Zn concentrations by inductively coupled plasma mass spectrometry was produced over 2 yr in Beaumont, TX, under both flooded and unflooded watering regimes. The distributions of all element concentrations analyzed were skewed toward higher concentration. A significant portion of this ionomic variation has a genetic basis (broad sense heritabilities 0.14-0.75), indicating an ability to breed for improved grain concentration of all elements except possibly Ni. Variation in grain elemental concentrations was not strongly associated with plant height, heading time, or grain shape, suggesting these physiological factors are not of primary importance in controlling ionomic variation in rice grain. Accessions high in specific elements were sometimes found to have similar genetic or geographic origins, suggesting they share a heritable mechanism underlying their enhanced ionomes. For example, accessions with high Ca, Mg, or K were more common in the indica than in the japonica subgroup; low As was most common among temperate japonica accessions; and several lines high in Mo originated in Malaysia or adjacent Brunei.

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Section: Determination Of Element Concentrations In Unmilled Rice Grainmentioning

confidence: 96%

Worldwide Genetic Diversity for Mineral Element Concentrations in Rice Grain

et al. 2015

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“…While human zinc absorption and transport kinetics were characterized using three-dimensional Caco-2 models (Table 3), a two-dimensional culture of these cells was additionally applied to investigate zinc uptake parameters. Furthermore, this model was widely used to study the effects of various dietary food components on intestinal zinc bioavailability [96,243,247,[258][259][260][261][262][263][264][265][266][267][268][269][270][271][272] and to elucidate the regulatory role of intestinal zinc transporters and metallothionein in zinc absorption [74,86,87,144,242,[273][274][275][276][277][278][279][280]. Notably, the impact of dietary zinc on zinc transporters and [255], which represents about 90% of intestinal cells of the brush border membrane [256,257].…”

Section: Investigation Of Zinc Uptake and Transport Using In Vitro Cementioning

confidence: 99%

A Guide to Human Zinc Absorption: General Overview and Recent Advances of In Vitro Intestinal Models

2020

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Zinc absorption in the small intestine is one of the main mechanisms regulating the systemic homeostasis of this essential trace element. This review summarizes the key aspects of human zinc homeostasis and distribution. In particular, current knowledge on human intestinal zinc absorption and the influence of diet-derived factors on bioaccessibility and bioavailability as well as intrinsic luminal and basolateral factors with an impact on zinc uptake are discussed. Their investigation is increasingly performed using in vitro cellular intestinal models, which are continually being refined and keep gaining importance for studying zinc uptake and transport via the human intestinal epithelium. The vast majority of these models is based on the human intestinal cell line Caco-2 in combination with other relevant components of the intestinal epithelium, such as mucin-secreting goblet cells and in vitro digestion models, and applying improved compositions of apical and basolateral media to mimic the in vivo situation as closely as possible. Particular emphasis is placed on summarizing previous applications as well as key results of these models, comparing their results to data obtained in humans, and discussing their advantages and limitations.

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“…This suggests that lowering of PA enhances micronutrient molar ratio, and thus, possibly increasing the mineral bioavailability. Similarly, lowering of PA in wheat- Aegilops derivatives and Triticum monococcum enhances the bioavailability of zinc ( Salunke et al, 2012 ). The strong validity of the observed bioavailability of micronutrients with the phytate:mineral molar ratios in the lpa mutants reinforce the significance of utilizing these parameters for the micronutrient biofortification programs.…”

Section: Discussionmentioning

confidence: 99%

RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

et al. 2018

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Enhancement of micronutrient bioavailability is crucial to address the malnutrition in the developing countries. Various approaches employed to address the micronutrient bioavailability are showing promising signs, especially in cereal crops. Phytic acid (PA) is considered as a major antinutrient due to its ability to chelate important micronutrients and thereby restricting their bioavailability. Therefore, manipulating PA biosynthesis pathway has largely been explored to overcome the pleiotropic effect in different crop species. Recently, we reported that functional wheat inositol pentakisphosphate kinase (TaIPK1) is involved in PA biosynthesis, however, the functional roles of the IPK1 gene in wheat remains elusive. In this study, RNAi-mediated gene silencing was performed for IPK1 transcripts in hexaploid wheat. Four non-segregating RNAi lines of wheat were selected for detailed study (S3-D-6-1; S6-K-3-3; S6-K-6-10 and S16-D-9-5). Homozygous transgenic RNAi lines at T4 seeds with a decreased transcript of TaIPK1 showed 28–56% reduction of the PA. Silencing of IPK1 also resulted in increased free phosphate in mature grains. Although, no phenotypic changes in the spike was observed but, lowering of grain PA resulted in the reduced number of seeds per spikelet. The lowering of grain PA was also accompanied by a significant increase in iron (Fe) and zinc (Zn) content, thereby enhancing their molar ratios (Zn:PA and Fe:PA). Overall, this work suggests that IPK1 is a promising candidate for employing genome editing tools to address the mineral accumulation in wheat grains.

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Determination of bioavailable-zinc from biofortified wheat using a coupled in vitro digestion/Caco-2 reporter-gene based assay

Cited by 14 publications

References 47 publications

Worldwide Genetic Diversity for Mineral Element Concentrations in Rice Grain

Worldwide Genetic Diversity for Mineral Element Concentrations in Rice Grain

A Guide to Human Zinc Absorption: General Overview and Recent Advances of In Vitro Intestinal Models

RNAi-Mediated Downregulation of Inositol Pentakisphosphate Kinase (IPK1) in Wheat Grains Decreases Phytic Acid Levels and Increases Fe and Zn Accumulation

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