Determination of Chloride Concentration Using Capillary Zone Electrophoresis: An Instrumental Analysis Chemistry Laboratory Experiment

Abstract

Figure 1. Schematic of capillary electrophoresis system.

Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Diagnosis of Phenylketonuria: A Versatile Wet or Dry Laboratory Experiment in Upper-Level Undergraduate Analytical Chemistry

“…A number of undergraduate laboratory experiments utilizing CE have been developed subsequent to the availability of CE instrumentation analyzing various small molecules, − inorganic ions, − and biopolymers. ,− While effective in demonstrating the use of CE across a wide range of compounds, these experiments utilize UV absorbance almost exclusively as a means of detection while neglecting to utilize other detection methods. To our knowledge, an undergraduate laboratory experiment using CE-LIF has yet to be published.…”

Capillary Zone Electrophoresis for the Analysis of Peptides: Fostering Students’ Problem-Solving and Discovery Learning in an Undergraduate Laboratory Experiment

“…Indeed, CE is routinely used in research applications for the analysis of peptides. − Microfluidic CE devices are often connected to electrospray mass spectrometers for proteomic analysis. − Capillary electrophoresis is covered in most analytical textbooks, and several laboratory experiments have been developed to demonstrate the power of this technique . These include laboratories aimed at determining the amount of caffeine in various foods and beverages, − separation of barbiturates, analysis of cations in soil and water samples, − studying the reaction products from an organic chemistry experiment, , the analysis of various foods and food dyes, and analgesic formulations . Laboratories with biological applications include determining protein amino acid composition, determining the amino acid composition of a dipeptide using the related micellar electrokinetic capillary chromatography technique, carbohydrate analysis, and determining the diffusion constant of dopamine .…”

Determination of a Diffusion Coefficient by Capillary Electrophoresis. An Experiment for the Physical and Biophysical Chemistry Laboratories

“…Over the past two decades, capillary electrophoresis (CE) has become a key tool for high resolution chemical separations of a wide variety of compounds (1,2). Study of CE is now an essential component of analytical chemistry lecture courses, and a number of instructional laboratory exercises have been described in previous issues of this Journal (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Those experiments focused on the primary function of CE as a separation tool in the analytical laboratory.…”