Determination of clonidine in human plasma by gas chromatography—electron-impact mass spectrometry

Yamahata, Tohru; Dote, Sumiko; Ozawa, Yukihiko; Nishikawa, Hitoshi; Maeda, Seiji

doi:10.1016/0378-4347(93)e0417-o

Cited by 16 publications

(17 citation statements)

References 12 publications

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“…Numerous analytical methods, including radioimmunoassay (Arndts et al, 1981), HPLC (Rosansky et al, 1993), GC (Chu et al, 1979;Edlund, 1980;Nazarali et al, 1986;Velasquez et al, 1983), GC/MS (Arrendale et al, 1988;Girault and Fourtillan, 1988;Murray et al, 1981;Murray and Davies, 1984;Wenzl et al, 2002;Yamahata et al, 1994), LC-MS (Ke et al, 2004) and LC-MS/MS (Muller et al, 2007;Naidong et al, 2002) have been reported in the literature to quantify clonidine in biological fluids. None of these methods could reliably quantify clonidine concentration lower than 50 pg/ mL.…”

Section: Introductionmentioning

confidence: 98%

Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma

Nirogi

Kandikere

Mudigonda

et al. 2008

Biomedical Chromatography

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A sensitive high-performance liquid chromatography-positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of clonidine in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H](+) ions, m/z 230 to 44 for clonidine and m/z 254 to 44 for the internal standard. The assay exhibited a linear dynamic range of 10-2000 pg/mL for clonidine in human plasma. The lower limit of quantification was 10 pg/mL with a relative standard deviation of less than 6.8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 250 human plasma samples per day. The validated method was successfully used to analyze human plasma samples for application in pharmacokinetic studies.

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Section: Introductionmentioning

confidence: 98%

Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma

Nirogi

Kandikere

Mudigonda

et al. 2008

Biomedical Chromatography

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“…Sampling time-points were set at 0, 3,6,12,24,48,72,96,120,144,168,192,216, and 240 h. The patch was removed after 168 h. Blood samples were collected from the ear vein. Plasma samples were separated by centrifugation (3500 rpm) for 10 min, processed using the solid extraction cartridge for extraction of clonidine as described above, and analyzed using the LC-MS method.…”

Section: Measurement Of Clonidine In Plasma Using Lc-msmentioning

confidence: 99%

“…Several quantification methods were reported for the measurement of the clonidine plasma concentration, including combined gas chromatography/electron-capture negative-ion chemical ionization mass spectrometry 5,6) and radioimmunoassay (RIA).…”

mentioning

confidence: 99%

In Vitro and in Vivo Characterization of a Newly Developed Clonidine Transdermal Patch for Treatment of Attention Deficit Hyperactivity Disorder in Children

Wang²,

Xue³

et al. 2005

Biological & Pharmaceutical Bulletin

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“…Certain withdrawal symptoms such as a transient increase in blood pressure and tachycardia are not observed in the present study. These symptoms have been observed by a higher dose (250 pg/kg/day) of clonidine that elicited more remarkable hypotension with higher plasma levels exceeding 3 ng/ml (13). Thus, the withdrawal symptoms by clonidine may be dependent on its plasma levels which are related to its hypotensive effects.…”

mentioning

confidence: 98%

“…Plasma samples were isolated by centrifugation (1500 x g for 15 min at 41C) and stored at -801C until assayed . Analyses of plasma clonidine concentrations were performed by gas chromatographymass spectrometry (GC/MS) (Model 5890, Series 2 and Model 5971A; Hewlett-Packard, North Hollywood, CA, USA) (13). Data are expressed as means±S.E.…”

mentioning

confidence: 99%

Absence of Tolerance to Hypotensive Effects of Clonidine in Spontaneously Hypertensive Rats

Naruse

Ishii

Tagawa

1995

Japanese Journal of Pharmacology

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ABSTRACT-Development of tolerance to the hypotensive effects of clonidine was investigated in spontaneously hypertensive rats (SHR). Clonidine (125 pg/kg/day) was administered subcutaneously for 5 weeks using an osmotic infusion pump. During the whole infusion period, significant hypotensive and bradycardic effects were observed. Plasma clonidine concentrations were maintained relatively constant at about 2 ng/ml during the infusion period. On termination of treatment with clonidine, both the blood pressure and heart rate rapidly recovered to the control levels. These findings suggest that clonidine does not cause tolerance to its hypotensive effects in SHR with the present administration regimen.

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Determination of clonidine in human plasma by gas chromatography—electron-impact mass spectrometry

Cited by 16 publications

References 12 publications

Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma

Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma

In Vitro and in Vivo Characterization of a Newly Developed Clonidine Transdermal Patch for Treatment of Attention Deficit Hyperactivity Disorder in Children

Absence of Tolerance to Hypotensive Effects of Clonidine in Spontaneously Hypertensive Rats

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