Determination of dihydroergotamine in human plasma by high-performance liquid chromatography with fluorescence detection

Murday, Michelle; McLean, Angus M.; Slaughter, Elizabeth; Couch, Richard A.

doi:10.1016/s0378-4347(99)00405-3

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…It is used also for the acute treatment of the headache phase of migraine attacks [15]. DHE, a semisynthetic ergopeptide, is widely used to prevent or treat vascular headaches such as migraine and cluster headaches [16, 17]. It acts by the stimulation of serotonergic receptors of neurons of the capacitance vessels [18, 19].…”

Section: Introductionmentioning

confidence: 99%

“…Various analytical procedures have been implemented for the determination of these compounds in pharmaceutical formulations and plasma samples. High-performance liquid chromatography (HPLC) with ultraviolet-visible detection [22–24], HPLC with fluorescence detection [16, 17, 25, 26], HPLC with coulometric detection [27, 28], and HPLC with mass spectroscopy [29–35] are the most used techniques. Additionally, a micellar electrokinetic capillary chromatography has been developed and validated to allow the analysis of Nara in pharmaceutical products [36].…”

Section: Introductionmentioning

confidence: 99%

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Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Jebali

Belgacem

Louhaichi

et al. 2019

International Journal of Analytical Chemistry

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A sensitive, precise, accurate, and specific isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous separation and determination of zolmitriptan, naratriptan, dihydroergotamine, ketotifen, and pizotifen in pharmaceutical formulations has been developed and validated. An experimental design was applied for the optimization of the chromatographic parameters. A two-level full factorial 2k was used for studying the interaction between the variables to be optimized: the percentage of acetonitrile in the mobile phase, mobile-phase pH, nature of the buffer, and column oven temperature. The most significant parameters are the percentage of acetonitrile and the mobile-phase pH. These significant parameters were optimized using the Doehlert matrix. The optimum separation was achieved by means of a Waters XBridge C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile and a 10 mM sodium perchlorate buffer (38 : 62, v/v) at a flow rate of 1.0 mL·min−1 and UV detection at 220 nm. The selectivity, method linearity, accuracy, and precision were examined as part of the method validation. The described method shows excellent linearity over a range of 30 to 70 μg·mL−1 for all compounds with correlation coefficients higher than 0.995. The standard deviations of the intraday and interday precision were between 0.75 and 1.94%. The validated method was successfully applied to perform routine analysis of these compounds in different pharmaceutical products such as syrups and tablets. In the presence of some preservatives, it was found that there were no peaks at the related peak locations.

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