Determination of diprivan in urine by a supported liquid membrane technique and liquid chromatography-electrochemical detection

Trocewicz, Jerzy; Suprynowicz, Z.; Markowicz, Joanna

doi:10.1016/0378-4347(96)00149-1

Cited by 19 publications

(11 citation statements)

References 11 publications

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“…The SLM system consisted of an infusion pump (Micro 505, 3M, USA) and a home-made teflon spiral membrane separator of 0.75 mL acceptor volume (Trocewicz et al, 1996). A microporous 0.2 mm teflon membrane utilized for separations (Millipore, Bedford, MA, USA) was saturated with n-undecane.…”

Section: Analytical Equipmentmentioning

confidence: 99%

“…A 0.1 M NaOH solution was used as acceptor phase. Other conditions of the SLM enrichment procedure are described by Trocewicz et al (1996). After the enrichment process, the alkaline acceptor phase was neutralized with 5 M sulphuric acid to protect the RP column packing material from damage.…”

Section: Sample Preparationmentioning

confidence: 99%

“…ropi-vacaine and its metabolites (Jönsson et al, 2000). For propofol, the conditions of SLM isolation and preconcentration have been optimized for the drug assay in spiked samples of urine (Trocewicz et al, 1996), giving a drug determination limit as low as 10 ppt. In order to investigate the amount of propofol excreted in urine the SLM technique was applied in this study for propofol isolation.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of propofol renal elimination by HPLC using supported liquid membrane procedure for sample preparation

Dawidowicz¹,

Kalitynski²,

Trocewicz³

et al. 2002

Biomedical Chromatography

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One of the least explored subjects in the research on the metabolism of a widely used anaesthetic, propofol, is its excretion in an unchanged form. According to literature, the estimated percentage of applied propofol eliminated intact via kidneys is lower than 0.3%. The present study shows the amount of propofol excreted in an unchanged form with urine collected during the first 48 h after anaesthesia in five patients undergoing elective intracranial procedures. The drug was concentrated and selectively isolated from urine samples by supported liquid membrane technique and determined by HPLC with fluorescence detection. The amount of unchanged propofol eliminated with urine was approximately (0.004 +/- 0.002)% of the total applied dose. The obtained results may suggest that propofol in an unchanged form is not excreted by kidneys at all provided that all propofol determined in presented study originated from conjugates hydrolysis.

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Section: Analytical Equipmentmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of propofol renal elimination by HPLC using supported liquid membrane procedure for sample preparation

Dawidowicz¹,

Kalitynski²,

Trocewicz³

et al. 2002

Biomedical Chromatography

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“…These procedures have generally employed reversed phase High Performance Liquid Chromatography (RP-HPLC) with either UV, 4 fluorimetric [5][6][7] or electrochemical 8,9 detection techniques. The mobile phase was usually composed of a relatively high proportion of an organic solvent (e. g. acetonitrile or methanol) and an acidic aqueous buffer in Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Multi-wavelength spectrophotometric determination of propofol acidity constant in different acetonitrile-water mixtures

Pissinis

Sereno

Marioli

2005

J. Braz. Chem. Soc.

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A constante ácido-base de uma droga anestésica, propofol, foi determinada em diferentes misturas acetonitrila-água usando-se dados de titulação espectrofotométrica multivariada. Os valores de pH medidos com eletrodo de vidro calibrado com tampão aquoso, nas misturas acetonitrila-água, s w pH, foram corrigidos para obter-se o s s pH, que é o pH da mistura acetronitrila-água medido com eletrodo de vidro, calibrado com tampão na mesma mistura de solvente. Essa escala de pH está diretamente relacionada com a constante termodinâmica ácido-base. O valor de pK a do propofol encontrado em água é de 11,67. Relações lineares foram observadas quando os valores calculados de pK a do propofol, em diferentes misturas de solvente, foram comparados com a fração molar de acetonitrila. Uma relação linear foi também encontrada entre os valores de pK a e a permissividade relativa recíproca da mistura de solventes, 1/ε. Este comportamento foi considerado como indicativo da natureza eletrostática do processo de ionização.The acid-base constant of an anaesthetic drug, propofol, was determined in different acetonitrilewater mixtures by using multiwavelength spectrophotometric titration data. The pH values measured in acetonitrile-water mixtures with a glass electrode calibrated with an aqueous buffer, s w pH, were corrected to obtain s s pH, that is, the pH of the acetonitrile-water mixture as measured with a glass electrode calibrated with a buffer in the same solvent mixture. This last pH scale is directly related to the thermodynamic acid-base constant. The pK a value of propofol in pure water was found to be 11.67. Linear relationships were observed when the calculated pK a values of propofol in different solvent mixtures were plotted against the acetonitrile molar fraction. Also, a linear relationship was observed between pK a values and the reciprocal of the relative permittivity of the solvent mixture, 1/ε. This behavior was taken as indicative of the electrostatic nature of the ionization process.

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“…High-performance liquid chromatography (HPLC) is one of the most extensively and frequently used analytical techniques for determining the concentration of the drug in body fluids. Deproteinization and/or extraction are essential in conventional analysis before biological samples can be applied to an HPLC system (Vree et al, 1987;Plummer, 1988;Mazzi and Schinella, 1990;Pavan et al, 1992;Dawidowicz and Fijalkowska, 1995;Trocewicz et al, 1996 andYeganeh andRamzan, 1997). These clean-up procedures often take up the majority of the total analysis time and contribute significantly to the final cost of the analysis, both in terms of labour and the consumption of materials.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic assay and pharmacokinetic studies of propofol in human serum

Emara

Saleh

Fathy

et al. 1999

Biomed. Chromatogr.

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Determination of diprivan in urine by a supported liquid membrane technique and liquid chromatography-electrochemical detection

Cited by 19 publications

References 11 publications

Investigation of propofol renal elimination by HPLC using supported liquid membrane procedure for sample preparation

Investigation of propofol renal elimination by HPLC using supported liquid membrane procedure for sample preparation

Multi-wavelength spectrophotometric determination of propofol acidity constant in different acetonitrile-water mixtures

Chromatographic assay and pharmacokinetic studies of propofol in human serum

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