Determination of doxepin and desmethyldoxepin in human plasma using liquid chromatography–tandem mass spectrometry

Badenhorst, D.; Sutherland, Frances; Jager, A.D de; Scanes, T; Hundt, H. K. L.; Swart, K.J; Hundt, A.F

doi:10.1016/s0378-4347(00)00136-5

Cited by 25 publications

(20 citation statements)

References 5 publications

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“…BenzoctamineHCl was used as IS. Linearity was observed in the range 81.1-0.320 ng/mL for doxepin and 45.1-0.178ng/mL for desmethyldoxepin [56].…”

Section: Chromatographic Conditionsmentioning

confidence: 92%

“…Other detection techniques were also used by some researchers: Electrochemical detection [43,[50][51][52][53], fluorescence detection after post-column photochemical reaction [37], Chemiluminescent detection using 0.75mM Ru(bipy) 3 Cl 2 solution [54] and MS/MS [55][56][57][58][59].…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…Mixtures of hexane with isoamyl alcohol in different ratios (98:2 or 99:1) were used for the extraction of desipramine from rabbit plasma and rabbit bone marrow (with low recovery rates: 24.2-30.2% in rabbit plasma and 24.5-34.2% in rabbit bone marrow) [27], as well as for the extraction of different TCAs from human plasma: imipramine, desipramine, amitriptyline and nortriptyline (with recovery 89.6-103.2%) [33], amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, doxepin and trimipramine [38], doxepin, and desmethyldoxepin using (with recovery of 90% (doxepin) and 75% (desmethyldoxepin) [56], amitriptyline, clomipramine, desipramine, doxepin, imipramine, noxiptyline and opipramol (recovery 75-90% except for amitriptyline (67%) and opipramol (47%) [46] and imipramine, amitriptyline and nortriptyline (recovery 97.0-103.9%) [67].…”

Section: Sample Preparationmentioning

confidence: 99%

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HPLC as a Tool in Medicinal Chemistry for the Monitoring of Tricyclic Antidepressants in Biofluids

Samanidou¹,

Nika²,

Papadoyannis³

2008

MRMC

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“…BenzoctamineHCl was used as IS. Linearity was observed in the range 81.1-0.320 ng/mL for doxepin and 45.1-0.178ng/mL for desmethyldoxepin [56].…”

Section: Chromatographic Conditionsmentioning

confidence: 92%

Section: Chromatographic Conditionsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

See 1 more Smart Citation

HPLC as a Tool in Medicinal Chemistry for the Monitoring of Tricyclic Antidepressants in Biofluids

Samanidou¹,

Nika²,

Papadoyannis³

2008

MRMC

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show abstract

“…Linearity was observed in the range 81.1-0.320 ng/mL for doxepin and 45.1-0.178 ng/mL for desmethyldoxepin [58]. Benzoctamine-HCl was used as IS.…”

Section: Hplc Analysis Of Tricyclic Antidepressants Recent Advances Imentioning

confidence: 99%

HPLC and its Essential Role in the Analysis of Tricyclic Antidepressants in Biological Samples

Samanidou¹,

Nika

Papadoyannis

2014

Recent Advances in Medicinal Chemistry

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“…However, the most recent publications described analytical methods for a larger number of antidepressants. 3,5,8,12,[14][15][16]28 They were based on different solid-phase or liquid-liquid extraction procedures and HPLC-UV, [3][4][5][6][7][8][9][10][11][12][13] HPLC coupled to single [16][17][18] or tandem mass spectrometry, [19][20][21][22] or gas chromatography with nitrogen phosphorous (NPD) 3,[23][24][25] or mass spectrometry detection. [26][27][28][29] On-line extraction and analysis of fluoxetine and its metabolite by column-switching HPLC coupled to mass spectrometry has recently been published.…”

mentioning

confidence: 99%

A Fully Automated Turbulent-Flow Liquid Chromatography-Tandem Mass Spectrometry Technique for Monitoring Antidepressants in Human Serum

Sauvage

Gaulier²,

Lachâtre³

et al. 2006

Therapeutic Drug Monitoring

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Antidepressants belong to a variety of chemical and pharmacologic classes. Most require therapeutic drug monitoring, at least in certain circumstances, such as unexplained inefficacy or suspected toxicity. Several types of chromatographic methods have generally been used. This paper presents a fully automated, sensitive, and specific method for the therapeutic drug monitoring of 13 antidepressants of all classes (amoxapine, amitriptyline, citalopram, clomipramine, dothiepin, doxepin, fluoxetine, imipramine, maprotiline, mianserin, paroxetine, sertraline, trimipramine) and some of their respective active metabolites (nortriptyline, monodesmethylcitalopram, desmethylclomipramine, desipramine, norfluoxetine, desmethylmianserin, N-desmethylsertraline), based on the innovative turbulent-flow liquid chromatography (TFC) technology, coupled to tandem-mass spectrometry (MS/MS). The antidepressants were divided in two groups depending on their chromatographic properties, so that two injections would be necessary to screen all compounds (which is infrequent for therapeutic drug monitoring). Calibration curves ranged from 10 to 500 ng/mL. No significant memory effect was observed after the injection of a blank serum sample spiked at 500 ng/mL. The intra-assay and inter-assay precision CVs ranged from 0.4% to 12% and from 1% to 16%, respectively. The method was further validated by blindly analyzing Heathcontrol-Therapeutic Drugs Scheme samples (Cardiff Bioanalytical Services Ltd.) containing several antidepressants.

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Determination of doxepin and desmethyldoxepin in human plasma using liquid chromatography–tandem mass spectrometry

Cited by 25 publications

References 5 publications

HPLC as a Tool in Medicinal Chemistry for the Monitoring of Tricyclic Antidepressants in Biofluids

HPLC as a Tool in Medicinal Chemistry for the Monitoring of Tricyclic Antidepressants in Biofluids

HPLC and its Essential Role in the Analysis of Tricyclic Antidepressants in Biological Samples

A Fully Automated Turbulent-Flow Liquid Chromatography-Tandem Mass Spectrometry Technique for Monitoring Antidepressants in Human Serum

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