Determination of flavonoids as complexes with Al<sup>3+</sup> in microemulsion media by HPLC method with fluorescence detection

Пирогов, А. В.; Sokolova, Lidia; Sokerina, Ekaterina; Tataurova, O. G.; Шпигун, О. А.

doi:10.1080/10826076.2016.1147462

Cited by 12 publications

(3 citation statements)

References 9 publications

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“…The trifluoroacetic acid-methanol solution was dried at 40°C to obtain the anthocyanins. [23,24] HPLC method for determining the content of blueberry anthocyanin in microemulsion As established in our laboratory, HPLC was used to determine the anthocyanin content in the microemulsion. [25] The specific conditions were as follows: mobile phase (acetonitrile:water, which contains 0.1% trifluoroacetic acid), 75:25; flow rate, 1 mL/min; and column temperature, 30°C.…”

Section: Transmission Electron Microscopic Analysis Of Anthocyanin MImentioning

confidence: 99%

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Chen

Hui

Yao

et al. 2018

International Journal of Food Properties

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Anthocyanins exhibit natural antioxidants but exhibit poor stability and low oil solubility. Therefore, increasing the stability and fat solubility of anthocyanins is necessary to broaden their applications. In our experiment, an anthocyanin microemulsion system was constructed and its stability was investigated. At an oil phase and emulsifier ratio of 7:3, the hydrophilic oil-wet value reached 7.5 and the Km was 2:1. Furthermore, the microemulsion area was the largest. The bicontinuous region of the microemulsion exhibited optimal experimental conditions and resulted in a microemulsion containing 425.54 ± 1.58 μg/g anthocyanins. The anthocyanin microemulsion was a water-in-oil type, with a particle size of 0.07 ± 0.009 μm. Moreover, the temperature, light, and sample observation tests showed that the anthocyanin retention rate in the microemulsion was higher than that in the anthocyanin solution. Low-concentration NaCl and less than 9% of glucose and sucrose exerted no significant influence on the anthocyanin microemulsion stability.

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Section: Transmission Electron Microscopic Analysis Of Anthocyanin MImentioning

confidence: 99%

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Chen

Hui

Yao

et al. 2018

International Journal of Food Properties

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“…A breakthrough in the intensification of the extraction process of compounds significant for the medical industry is the application of the achievements of genetic engineering that combine in vitro tissue culture and molecular biology methods [ 2 , 17 , 20 ].…”

Section: Genetic Engineering and Biotechnology In The In Vitro Promentioning

confidence: 99%

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

et al. 2020

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This paper studies modern methods of producing and using callus, suspension cells and root cultures of medicinal plants in vitro. A new solution for natural product production is the use of an alternative source of renewable, environmentally friendly raw materials: callus, suspension and root cultures of higher plants in vitro. The possibility of using hairy root cultures as producers of various biologically active substances is studied. It is proven that the application of the genetic engineering achievements that combine in vitro tissue culture and molecular biology methods was groundbreaking in terms of the intensification of the extraction process of compounds significant for the medical industry. It is established that of all the callus processing methods, suspension and root cultures in vitro, the Agrobacterium method is the most widely used in practice. The use of agrobacteria has advantages over the biolistic method since it increases the proportion of stable transformation events, can deliver large DNA segments and does not require special ballistic devices. As a result of the research, the most effective strains of agrobacteria are identified.

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“…Total flavonoids (in terms of rutin) in the plants were determined using the spectrophotometric method with a UV 3600 spectrophotometer (Shimadzu, Kyoto, Japan). The method [ 35 ] involves the spectrophotometric detection of flavanol complexes with aluminum chloride at a wavelength of 410-413 nm. Rutin was used as the internal standard.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

Babich

Сухих

Pungin

et al. 2021

Plants

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The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.

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Determination of flavonoids as complexes with Al³⁺ in microemulsion media by HPLC method with fluorescence detection

Cited by 12 publications

References 9 publications

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

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Determination of flavonoids as complexes with Al3+ in microemulsion media by HPLC method with fluorescence detection

Cited by 12 publications

References 9 publications

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Microemulsion-based anthocyanin systems: effect of surfactants, cosurfactants, and its stability

Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants

Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

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Determination of flavonoids as complexes with Al³⁺ in microemulsion media by HPLC method with fluorescence detection