Determination of free malondialdehyde in human serum by high-performance liquid chromatography

Karataş, Fikret; Karatepe, Mustafa; Baysar, Ahmet

doi:10.1016/s0003-2697(02)00387-1

Cited by 259 publications

(184 citation statements)

References 12 publications

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“…This study used HPLC, which has great sensitivity and specificity by separating contaminant complexes in specific columns (Karatas et al, 2002). Other studies used spectrophotometry.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Zuluaga

2016

Rev Colomb Cienc Pecu

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Concentración de óxido nítrico y malonaldehído en contenido gástrico y en sangre en un modelo de úlcera gástrica equina inducida por fenilbutazona Concentração de óxido nítrico e malondialdeído no conteúdo gástrico e no sangue em modelo equino de úlcera gástrica Summary Background: mechanisms of gastric mucosal injury include cellular damage by oxygen free radicals, which can be indirectly measured through malondialdehyde (MDA). Production of nitric oxide (NO) maintains gastric tissue perfusion through vasodilatation. Objective: to demonstrate oxidative stress and impaired gastric perfusion measuring NO and MDA in gastric contents and blood of equines subjected to a gastrointestinal ulceration induction protocol. Methods: a gastrointestinal ulceration induction protocol involving fasting and oral administration of phenylbutazone was performed on five horses. NO and MDA were measured before and after protocol induction and presence of fecal occult blood (FOB) was evaluated. Animals underwent gastroscopy at the beginning and end of the protocol. Results: horses presented variability in hematological and FOB exams. Azotemia, hyperphosphatemia, and hypocalcemia were found in all animals. No significant changes were found in enzymatic activity. At the end of the protocol, 40% of the horses showed varying degrees of gastric ulceration. NO production in the stomach decreased by 60%, whereas MDA production increased by 55% after the protocol. Plasma concentration of MDA average increased 96 hours after starting the protocol. There were no significant differences in mean plasma NO during the protocol. Conclusion: the

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“…This study used HPLC, which has great sensitivity and specificity by separating contaminant complexes in specific columns (Karatas et al, 2002). Other studies used spectrophotometry.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Zuluaga

2016

Rev Colomb Cienc Pecu

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“…Mobile phase consists of 30 mmol KH2PO4 and methanol (65%-35%, H3PO4 by pH 4), and the mobile phase at a 1.5 mL/min. flow rate, wavelength 250 nm according to the method of Karatas et al 2002(Karatas et al, 2002. Data Presented in Fig.3.…”

Section: Determination Of Mda By Hplcmentioning

confidence: 99%

Neuroprotective effects of herbal cocktail on cerebrovascular dysfunction in rats with induced hyperhomocysteinaemia

Algohary¹,

Ahmad-Farid

Abd-Elrazek

et al. 2016

Biomed Res Ther

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Introduction: Hyperhomocysteinaemia (HHcy) is an established risk factor for cardiovascular, cerebrovascular, peripheral vascular diseases and neurodegenerative disease. The effect of this HHcy on vascular diseases could potentially cause vascular pathology features. Experimental studies have demonstrated that Hcy can be neurotoxic to brain, hippocampus area. Methods: The present study was conducted to compare the possible neuroprotective effects of different herbal cocktail in HHcy-induced rats' brain cerebrovascular dysfunction model. Rats were divided into nine groups: Group IControls received the same volume of saline solution (0.5 mL/100 g of body weight).

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“…Apart from the above mentioned method, other methods were applied for the quantitative assay of lipoperoxidation end-products: direct HPLC [Karatas et al, 2002], capillary electrophoresis [Wilson et al, 1997], RP-HPLC, derivatisation with 2,4-diphenylhydrazine [Sim et al, 2003], pre-column derivatisation with diaminonaphtalene at acidic pH (for protein bound MDA) or alkaline pH (for non protein-bound MDA), followed by HPLC-UV…”

Section: Evaluation Of the End Products Of Lipid Peroxidationmentioning

confidence: 99%

Trends in the Evaluation of Lipid Peroxidation Processes

Ilie¹,

Margină²

2012

Lipid Peroxidation

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Determination of free malondialdehyde in human serum by high-performance liquid chromatography

Cited by 259 publications

References 12 publications

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Neuroprotective effects of herbal cocktail on cerebrovascular dysfunction in rats with induced hyperhomocysteinaemia

Trends in the Evaluation of Lipid Peroxidation Processes

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