Determination of Fumonisin B1 in animal tissues with immunoaffinity purification

Tardieu, Didier; Auby, Aliénor; Bluteau, Caroline; Bailly, Jean‐Denis; Guerre, Philippe

doi:10.1016/j.jchromb.2008.05.046

Cited by 27 publications

(11 citation statements)

References 15 publications

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“…The columns usually used to purify the samples are of the SAX or C18 type, whereas immunoaffinity (IA) columns are rarely used in UHPLC-MS/MS analysis, except to clean some plant samples [31,32]. IA columns have been used with HPLC analysis and fluorescent detection for quantitation of FB1 in liver, but the relatively low sensitivity of fluorescence detection did not enable quantitation of other FB than FB1, nor quantitation of FB1 in muscles [33].…”

Section: Introductionmentioning

confidence: 99%

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Tardieu

Travel

Métayer

et al. 2019

Toxins

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Although provisional maximum tolerable daily intake and recommended guidelines have been established for fumonisins (FB) in food, few data are available concerning levels of FB in edible animal tissues. Such data are of particular interest in avian species that can tolerate relatively high levels of fumonisins in their feed. Also, even if multiple contamination of animal feed by toxins produced by Fusarium is very frequent, little is known about the consequences of multiple contamination for FB levels in tissues. The aim of this study was to analyze the concentrations of FB in the muscle and liver of chickens and turkeys fed with FB alone and with FB combined with deoxynivalenol (DON), and with zearalenone (ZEN). Experimental diets were formulated by incorporating ground cultured toxigenic Fusarium strains in corn-soybean based feeds. Control diets were free of mycotoxins, FB diets contained 20 mg FB1+FB2/kg, and FBDONZEN diets contained 20, 5, and 0.5 mg/kg of FB1+FB2, DON, and ZEN, respectively. Animals were reared in individual cages with free access to water and feed. The feed was distributed to male Ross chickens from the 1st to the 35th day of age and to male Grade Maker turkeys from the 55th to the 70th day of age. On the last day of the study, the birds were starved for eight hours, killed, and autopsied for tissues sampling. No sign of toxicity was observed. A UHPLC-MS/MS method with isotopic dilution and immunoaffinity clean-up of samples has been developed for analysis of FB in muscle (n = 8 per diet) and liver (n = 8 per diet). Only traces of FB that were below the LOQ of 0.25 µg/kg were found in most of the samples of animals fed with the control diets. Mean concentrations of FB1, FB2, and FB3 in muscle were 17.5, 3.39, and 1.26 µg/kg, respectively, in chickens, and 5.77, 1.52, and 0.54 µg/kg in turkeys, respectively. In the liver, the respective FB1, FB2, and FB3 concentrations were 44.7, 2.61, and 0.79 µg/kg in chickens, and 41.47, 4.23, and 1.41 µg/kg, in turkeys. Cumulated level of FB1+FB2+FB3 in the highly contaminated samples were above 60 and 100 µg/kg in muscle and liver, respectively. The concentrations of FB in the tissues of animals fed the FBDONZEN diet did not greatly differ from the concentrations measured in animals fed the diet containing only FB.

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Section: Introductionmentioning

confidence: 99%

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Tardieu

Travel

Métayer

et al. 2019

Toxins

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“…Immunoaffinity clean‐up step is commonly employed to prepare the samples for mycotoxin analysis. Antibodies bonded to beds of IAC (immune affinity columns) can create reversible links with toxin molecules that results in elimination of undesirable substances hence superior sample clean up . The method shows the high selectivity and good recovery but the costs of IAC columns are very high.…”

Section: Introductionmentioning

confidence: 99%

Application of molecularly imprinted polymers to determine B₁, B₂, and B₃ fumonisins in cereal products

Bryła¹,

Jędrzejczak

Roszko

et al. 2013

J of Separation Science

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The aim of this study was to develop an analytical method for qualitative and quantitative determination of the B(1), B(2,) and B(3) fumonisins in cereal products. A LC coupled to an IT-MS was used as the analytical instrument. The AFFINIMIP FumoZON Molecularly Imprinted Polymer SPE cartridges (Polyintell) were used to isolate fumonisins from the analyzed samples and the clean-up step. Statistical parameters evaluated in some validation experiments were as follows: mean recovery 95-106%, precision <17% (expressed as recovery RSD). The developed method was used to determine fumonisins in 49 cereals (42 maize-based and seven wheat-based products). In most cases, concentrations of the studied compounds found in the analyzed samples were low. The highest total concentration of the B(1), B(2), and B(3) fumonisins was found in maize flour samples (range, 26-1102 μg/kg, mean 498 μg/kg).

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“…The application of immunoaffinity chromatography for a variety of samples pretreatment in toxins, veterinary drugs and pesticides residues analyses have been reported [10][11][12][13][14][15][16]. In this work, we describe the generation and application of polyclonal antibody (pAb)-based immunoaffinity chromatography as a cleanup procedure, followed by enzyme-linked immunosorbent assay (ELISA) analysis of MA in animal tissue and feed samples.…”

Section: Introductionmentioning

confidence: 99%

Preparation of an immunoaffinity column and its application in sample cleanup for methandrostenolone residues detection

Wang

Zhang

et al. 2011

Journal of Chromatography B

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Determination of Fumonisin B1 in animal tissues with immunoaffinity purification

Cited by 27 publications

References 15 publications

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Application of molecularly imprinted polymers to determine B₁, B₂, and B₃ fumonisins in cereal products

Preparation of an immunoaffinity column and its application in sample cleanup for methandrostenolone residues detection

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Determination of Fumonisin B1 in animal tissues with immunoaffinity purification

Cited by 27 publications

References 15 publications

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Application of molecularly imprinted polymers to determine B1, B2, and B3 fumonisins in cereal products

Preparation of an immunoaffinity column and its application in sample cleanup for methandrostenolone residues detection

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Product

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Application of molecularly imprinted polymers to determine B₁, B₂, and B₃ fumonisins in cereal products