Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Sitanggang, Azis Boing; Wu, Ho‐Shing; Wang, Shaw S.

doi:10.1007/s12257-009-0105-0

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…Determination of fungal GlcN: Determination of fungal GlcN was carried out by means of HPLC and reported by our previous studies [5,14] wherein hydrochlorination process used conventional thermal method. The analytical HPLC column was a LiChrospher® 100 RP-18 endcappedcolumn (5 μm, 4 mm i.d.…”

Section: Introductionmentioning

confidence: 99%

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Hs¹,

Lin²

2017

J Food Process Technol

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Section: Introductionmentioning

confidence: 99%

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Hs¹,

Lin²

2017

J Food Process Technol

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“…The determination of fungal GlcN was carried out by means of HPLC, as reported in our previous studies [ 6 , 20 ], wherein the hydrochlorination process was used by a conventional thermal method. The analytical HPLC column was a LiChrospher ® 100 RP-18 column (5 μm, 4 mm (internal diameter) × 250 mm).…”

Section: Methodsmentioning

confidence: 99%

Kinetic Study of Glucosamine Production Using Aspergillus sydowii BCRC 31742 under Solid-State Fermentation

Peng

2020

Molecules

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In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.

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“…The determination of GlcN concentration was in accordance with the method reported by Sitanggang et al (2009) with some modifications. One gram of dried biomass was hydrolyzed with 10 mL of 6 N HCl at 100°C for 4 h. The hydrolyzed biomass was cooled to ambient temperature and 10 mL of deionized water was added.…”

Section: Extraction and Determination Of Glcn Concentration From Fungal Biomassmentioning

confidence: 99%

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

Chew

Lin

et al. 2021

CyTA - Journal of Food

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Glucosamine (GlcN) has been widely used to treat osteoarthritis. However, GlcN derived from crustacean shells has several drawbacks, and it is not vegan-friendly. Here, we produced GlcN from the biomass of Aspergillus oryzae NCH-42 and investigated the effects of environmental factors (e.g. carbon and nitrogen sources, temperature, and pH) on the GlcN production of the fungal biomass. The acidic stress resulted in significantly higher GlcN content in the cell wall of A. oryzae NCH-42. Moreover, the SEM examination revealed that the mycelial structure grown at pH 2.5 was thick and sturdy, compared to that at neutral pH conditions. The final yield of GlcN could be enhanced by up to 7.75-fold by cultivating at modified PDB with an initial pH of 2.5 at 30°C for 4 days. In this study, the GlcN content of A. oryzae biomass could reach up to 0.31 g/g biomass, indicating its high potentiality for GlcN production. Aumento de la producción de glucosamina a partir de Aspergillus oryzae NCH-42 mediante estrés ácido en fermentación sumergidaLa glucosamina (GlcN) se ha utilizado ampliamente para tratar la artrosis. Sin embargo, aquella que deriva de los caparazones de crustáceos presenta varios inconvenientes y no es apta para veganos. En el presente estudio produjimos GlcN a partir de la biomasa de Aspergillus oryzae NCH-42 e investigamos los efectos de los factores ambientales (por ejemplo, las fuentes de carbono y nitrógeno, la temperatura y el pH) en la producción de GlcN de la biomasa fúngica. El estrés ácido dio lugar a un contenido de GlcN significativamente mayor en la pared celular de A. oryzae NCH-42. Además, el examen SEM permitió comprobar que la estructura micelial cultivada a pH 2.5 era gruesa y robusta, en comparación con la cultivada en condiciones de pH neutro. El rendimiento final de GlcN se pudo multiplicar hasta por 7.75 al cultivarla en PDB (caldo de dextrosa de papa [patata]) modificado con un pH inicial de 2.5 a 30°C durante 4 días. En este estudio, el contenido de GlcN de la biomasa de A. oryzae alcanzó hasta 0.31 g/g de biomasa, lo que indica su alta potencialidad para producir GlcN.

show abstract

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Cited by 7 publications

References 47 publications

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Effect of Oxygen Transfer and Pellet Size for Producing of Glucosamine Using Aspergillus sydowii BCRC 31742 Cultivated in a Fermenter

Kinetic Study of Glucosamine Production Using Aspergillus sydowii BCRC 31742 under Solid-State Fermentation

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

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