Determination of Gallic Acid in Ayurvedic Polyherbal Formulation Triphala churna and its ingredients by HPLC and HPTLC

Meena, Ajay Kumar; Narasimhaji, Ch. V.; Velvizhi, D.; Singh, Arjun; Rekha, Punchappady Devasya; Kumar, Vijay; Ilavarasan, R.; Srikanth, Narayanam; Dhiman, K S

doi:10.5958/0974-360x.2018.00596.6

Cited by 21 publications

(9 citation statements)

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“…Instrumentation HPTLC analysis was performed on a CAMAG HPTLC system (Muttenz, Switzerland) equipped with an Automatic TLC sampler IV, twin trough development chamber, TLC Scanner 3 linked with WINCATS software version 1.4.4 [14,15,16,17].…”

Section: Hptlc and Hplc Analysismentioning

confidence: 99%

“…The mobile phase was consisted of Acetonitrile: Buffer (35:65) (v/ v) in an isocratic elution with flow rate of 1.8 ml/min 10 μl of the test sample (triplicate) was injected to HPLC system. The column temperature was kept 32 C. The detection of analytes at 227 nm was carried out by using Diode Array Detector (DAD) [17,18,19,20].…”

Section: Hplc Instrumentation and Chromatographic Conditionsmentioning

confidence: 99%

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Estimation of Withaferin-A by HPLC and standardization of the Ashwagandhadi lehyam formulation

Meena

Rekha

Perumal

et al. 2021

Heliyon

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Standardization is an important measurement for ensuring the quality control of herbal drugs. It has become essential to develop reliable, specific and sensitive quality control methods. Ashwagandhadi lehyam is an important Ayurvedic formulation containing Withania somnifera L., as one of the prime ingredient of formulation. The present study was undertaken to develop standardization parameters for Ashwagandhadi lehyam. Evaluation of various standardization parameters like organoleptic characters, Physico-chemical evaluation, HPTLC finger print profiling along with dominant bioactive markers and estimation of bioactive markers Withaferin-A by HPLC. The R f value of Withaferin-A 0.35 and Withanolide-A 0.45 is in formulation and reference standards were found comparable under UV light at 254 nm and 540 nm. The HPLC chromatogram of Ashwagandhadi lehyam and standard Withaferin-A showed at Rt of 5.015 and 5.050 min. The percentage of Withaferin-A was 0.092% present in Ashwagandhadi lehyam formulation. Bioactive markers are characteristic to the ingredients or botanicals to identify the presence of ingredients in formulation easily. The presence of bioactive markers is possible and its verification through the HPTLC fingerprint profile and quantification of biomarker by HPLC are the best ways to identify evaluate the quality of the finished formulation in the course of development of a standardization protocol for quality control of Ayurvedic formulation.

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Section: Hptlc and Hplc Analysismentioning

confidence: 99%

Section: Hplc Instrumentation and Chromatographic Conditionsmentioning

confidence: 99%

Estimation of Withaferin-A by HPLC and standardization of the Ashwagandhadi lehyam formulation

Meena

Rekha

Perumal

et al. 2021

Heliyon

Self Cite

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“…Triphala powder contains gallic acid, methyl gallate, chebulagic acid, chebulinic acid, chebulanin acid, chebulic acid, corilagin, bellericanin, beta-sitosterol, syringic acid, luteolin, rutin, rhamnose, kaempferol, gluconins, ellagic acid, phyllembic acid, quercetin, phyllantidine, sorbitol, and ascorbic acid [9] . Triphala mashi has been reported to contain tannins, saponins, gallic acid derivatives and ascorbic acid and has a pronounced concentration of gallic acid [ 10 , 11 ].…”

Section: Mashi Prepared From Plant Materialsmentioning

confidence: 99%

A review through therapeutic attributes of Ayurvedic formulation mashi

Joshi

Baheti

Wani

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Mashi is a black colored powder formulation obtained after combustion of the plant or animal drug. It is prepared by bahirdhum padhati (outside) or anterdhum padhati (in the close vessel). In this dosage form, bulk of raw material is reduced to a greater extent by the application of a certain quantum of energy. Due to this treatment, hidden chemical constituents become prominent and/or a new chemical moiety is formed which is therapeutically active. This formulation is cost-effective and easy to prepare. This review article aims to highlight the different mashi formulations mentioned in Ayurvedic text and also incorporate the formulation not mentioned in the Ayurvedic text but used by Ayurvedic practitioners. The objective was to introduce researchers to the simple yet excellent formulation mashi which should be studied in detail to establish its identity, purity, and therapeutic activity.

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“…After derivatization plate was scanned at 540 nm using tungsten lamp. The Rf values and fingerprint data were recorded by WIN CATS software [13][14].…”

Section: Hptlc Finger Print Profile Of Visamusthi Seedsmentioning

confidence: 99%

Comparative chemical profiling of purified and unpurified Strychnos nux-vomica Linn seeds: An attempt to reduce toxic Brucine content

Meena¹,

Venktaraman²,

Panda³

et al. 2021

IJAM

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Vishamusthi (Strychnos nux-vomica Linn.), a medicinal plant described as Upavisha (semi-poisonous) group of Ayurvedic Pharmacopoeia of India. Vishamusthi has widely been used and being practiced in several illness namely nervous debility, paralysis, weakness of limbs, sexual weakness, dyspepsia and etc. Ayurveda practices strictly recommend the use of Vishamusthi in therapeutics only after proper shodhana (purificatory procedure) through specific medias such as Gomutra (cow’s urine), Godugdha (cow’s milk), Goghrita (cow’s ghee), and etc. Although various shodhana procedures are recommended in Ayurvedic treatise, but updated scientific researches regarding the shodhana methods are lacking. The present study was undertaken to investigate the physicochemical and phytochemical parameters, quantitative estimation of brucine using cutting edge research tools such as high-performance thin layer chromatography (HPTLC), liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) analyses of Vishamusthi seeds before and after purification. The remarkable changes have been observed in different physicochemical parameters, HPTLC, HPLC, GC-MS and LC-MS chromatographic profiling before and after shodhana process of Vishamusthi seeds. Quantitative HPLC studies revealed that the process of shodhana resulted in depletion of toxic brucine (chief poisonous constituent of Vishamusthi seeds) reduced to 79.66% in chloroform extract and 64.54% in ethanol extract after shodhana process.

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Determination of Gallic Acid in Ayurvedic Polyherbal Formulation Triphala churna and its ingredients by HPLC and HPTLC

Cited by 21 publications

References 0 publications

Estimation of Withaferin-A by HPLC and standardization of the Ashwagandhadi lehyam formulation

Estimation of Withaferin-A by HPLC and standardization of the Ashwagandhadi lehyam formulation

A review through therapeutic attributes of Ayurvedic formulation mashi

Comparative chemical profiling of purified and unpurified Strychnos nux-vomica Linn seeds: An attempt to reduce toxic Brucine content

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