Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers

Goto, Susumu; Thakur, Ramesh; Ishii, Keisuke

doi:10.1007/s002990050555

Cited by 136 publications

(87 citation statements)

References 17 publications

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“…Extensive research has been conducted on genetic and epigenetic changes that may be attributed to the somaclonal variations during tissue culture. It was observed that presence or absence of variations during tissue culture depends upon the source of explant and the mode of regeneration (Goto et al 1998), including levels of growth substances that are used (Martin et al 2006). Plants regenerated from axillary buds or from other meristematic tissue showed the lowest tendency for genetic variation (Joshi and Dhawan 2007).…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Tiwari

Chandel

Gupta

et al. 2013

Physiol Mol Biol Plants

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The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. The microtubers were conserved for 1 year under three different culture media and consequently microplants were regenerated for the DNA analyses. During the study, a total of 38 (10 RAPD, 11 ISSR, 12 SSR and 5 AFLP) primers produced a total of 407 (58 RAPD, 56 ISSR, 96 SSR and 197 AFLP) clear, distinct and reproducible amplicons. Cluster analysis revealed 100 % genetic similarity among the mother plant and its derivatives within the clusters by SSR, ISSR and RAPD analyses, whereas AFLP analysis revealed from 85 to 100 % genetic similarity. Dendrogram analysis based on the Jaccard's coefficient classified the genotypes into five clusters (I-V), each cluster consisting of mother plant and its derivatives. Principal component analysis (PCA) also plotted mother plant and its genotypes of each cluster together. Based on our results, it is concluded that AFLP is the best method followed by SSR, ISSR and RAPD to detect genetic stability of in vitro conserved potato microtubers. The in vitro conservation medium (T2) is a safe method for conservation of potato microtubers to produce true-to-type plans.

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Section: Discussionmentioning

confidence: 99%

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Tiwari

Chandel

Gupta

et al. 2013

Physiol Mol Biol Plants

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“…Molecular marker analysis of micropropagated plants has been reported in several plant species (Bhatia et al 2011;Goto et al 1998;Salvi et al 2001;Varshney et al 2001). Martins et al (2004) reported the genetic stability of micropropagated almond plants.…”

Section: Discussionmentioning

confidence: 99%

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Khateeb

Bahar

Lahham

et al. 2012

Physiol Mol Biol Plants

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Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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“…지금 까지 Prunus dulcis의 RAPD 및 ISSR 분석 (Martins et al, 2004), Malus pumila의 RAPD 분석 (Modgil et al, 2005), Pinus thunbergii의 RAPD 분석 (Goto et al, 1998), 장기간 기 내 배양된 춘란(Cymbidium goeringii)과 한란(C. kanran)의 유전적 변이성 (Ryu et al, 2011 (Fang and Roose, 1997;Tsumura, 1996). 또한 AFLP (amplified fragment length polymorphism)보다 과정이 간단 하고 (Russel et al, 1997), RAPD보다는 primer 당 다형성 밴 드수가 더 많으며 (Iruela et al, 2002), primer들의 높은 결합 온도와 더 긴 서열 때문에 RAPD보다 정확하고 재현성이 우수한 밴드들이 생성되는 장점이 있다 (Esselman et al, 1999), 특히 ISSR이 목표로 하는 반복 서열은 진핵 게놈 전 체에 걸쳐 풍부하고 빠르게 변화하여 유전자 지문의 검출 에서 그 유용성이 입증되고 있다 (Godwin et al, 1997;Russel et al, 1997).…”

Section: 갈대(Phragmites Communisunclassified

Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius)

Ryu¹,

Kim²,

So³

et al. 2013

Korean Journal of Plant Resources

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-This study was carried out to address an efficient in vitro regeneration system from seed-derived callus of Phragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration via calli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6 supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 loci detected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of different in vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2 mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect genetic variation of regenerants.

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Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers

Cited by 136 publications

References 17 publications

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius)

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