Determination of glucosamine in impure chitin samples by high-performance liquid chromatography

Determination of Myriocin in Natural and Cultured Cordyceps cicadae Using 9-Fluorenylmethyl Chloroformate Derivatization and High-Performance Liquid Chromatography with UV-Detection

“…3 For the determination of most amino acids and aliphatic amines, some reports used mobile phase including KH2PO4 or boracic acid buffer and methanol or acetonitrile with complex gradient HPLC systems. 22,23 However, the separation was poor and the elution time was as long as 60 min not including the equilibration time between runs. In a preliminary study, it was found that separation was achieved on an Inertsil ® ODS-3 column (150 mm × 3.0 mm i.d.)…”

“…22,23 Different pH ranges (5 to 13) and media (water-acetonitrile, methanol-acetonitrile, methanol-THF, pyridine-THF) were examined. Except for pyridine-THF, derivatization did not occur regardless of reaction period and temperature.…”

The Efficacy of Glucosamine and Chondroitin Sulfate in the Treatment of Osteoarthritis: Are These Saccharides Drugs or Nutraceuticals?

“…The raw material is derived from chitin, a biopolymer present in the exoskeleton of marine intervertebrate animals. 18 There may be significant differences in purity and other phar- maceutical features among products even though the GS is processed only by a few companies. 19 Glucosamine sulfate, glucosamine hydrochloride and N-acetyl-glucosamine (Scheme 2) are commonly used alone or as part of the mixtures produced by the pharmaceutical industry.…”

Development of an Isotope-Dilution Flow-Injection Electrospray/ Mass Spectrometric Method for the Accurate Determination of Glucosamine in Pharmaceutical Formulation

“…2,5,6 However, typical UV/VIS detectors have shown limitation because glucosamine does not contain a chromophore to absorb UV/VIS light. Therefore, refractive index detectors have been employed for the HPLC analysis of glucosamine.…”

“…7,8 In addition to the detection difficulty, a relatively cumbersome normal phase LC separation is required for the direct analysis of glucosamine in pharmaceutical products. 2,[5][6][7][8] Pre-column derivatization has been employed to overcome the detection difficulty and/or to adopt a reverse phase LC separation. [9][10][11] Recently, a normal phase LC separation with ESI/MS detection method has been employed for the direct determination of glucosamine in human plasma.…”