Determination of heparin on intraocular lens surfaces by ion chromatography

Ander, Birgitta; Öhrlund, Åke

doi:10.1016/s0021-9673(01)00661-6

Cited by 17 publications

(10 citation statements)

References 7 publications

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“…A multicolor biosensor for heparin detection and quantification was developed on the basis of the fluorescence change of a water-soluble 2,1,3-benzothiadiazole-containing cationic conjugated polymer Liu, 2008, 2009). Determination of heparin was completed based on heparin coated poly(methyl methacrylate) (PMMA) intraocular lenses by ion chromatography (Ander et al, 2001). And a reversed-phase ion pair high-performance liquid chromatography (RPIP-HPLC) was developed for the separation of heparins using a C 18 column (Patel et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

Chen

et al. 2012

Biosensors and Bioelectronics

126

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Section: Introductionmentioning

confidence: 99%

Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

Chen

et al. 2012

Biosensors and Bioelectronics

126

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“…The amount of immobilized heparin was determined by hydrolyzing the heparin and quantifying the resulting glucosamine [ 12 ]. Typically, 1.0 × 10 7 heparin-immobilized microspheres were hydrolyzed in 250 μL of 4.8 M hydrochloric acid for 24 h at 98 °C in a sealed glass vial.…”

Section: Methodsmentioning

confidence: 99%

Heparin-immobilized microspheres for the capture of cytokines

Duo

Stenken

2010

Anal Bioanal Chem

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The preparation and characterization of heparin-immobilized microspheres which were used to bind acidic fibroblast growth factor (aFGF), vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1/CCL2), and regulation upon activation normal T-cell express sequence (RANTES/CCL5) is described. These beads were used as trapping agents in microdialysis sampling experiments in a separate study. Both free heparin and a synthesized heparin-albumin conjugate were immobilized onto microspheres and compared for their effectiveness. The heparin-albumin conjugate microspheres exhibited significant non-specific adsorption which appeared to be due to the albumin content. The prepared heparin-immobilized microspheres were stable for 3 months at 4 °C. A bead-based flow cytometric assay was developed to study the binding capacity and specificity of the heparin-immobilized microspheres to cytokines. These heparin-immobilized microspheres exhibited broad dynamic ranges for binding to the four cytokines (aFGF, 1.0–1000 ng/mL; VEGF, 0.5–1000 ng/mL; CCL2, 1.95–1000 ng/mL; CCL5, 1.95–500 ng/mL). Fast binding kinetics of the cytokines to the heparin-immobilized beads suggests that these beads may be useful as affinity agents in microfluidic flow systems.

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“…Microsphere amounts were confirmed using a hemacytometer. The amount of immobilized heparin was determined by hydrolyzing it followed by quantifying the resulting glucosamine [ 4 ].…”

Section: B Preparation Of Heparin-immobilized Microspheresmentioning

confidence: 99%

Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling

Duo

Cabrera

Stenken

2009

2009 IEEE/NIH Life Science Systems and Applications Workshop

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Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid.This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling.

show abstract

Determination of heparin on intraocular lens surfaces by ion chromatography

Cited by 17 publications

References 7 publications

Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide

Heparin-immobilized microspheres for the capture of cytokines

Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling

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