Determination of Huefner's factor and inactive hemoglobins in human, canine, and murine blood.

Abstract

Summary Determination of HUfner's factor and simultaneous measurement of met-and carboxyhemoglobin were performed in 31 human, 16 canine, and 2 murine blood samples. Both measured and corrected values of Hufner's factor were lower than the theoretical value of 1.39, suggesting the possibility of the presence of unidentifiable inactive hemoglobin(s) in blood.

Pharmaceutical preparation of oxygen-15 labelled molecular oxygen and carbon monoxide gasses in a hospital setting

“…As a trace of carrier oxygen is required in the target gas for good production yields of [ 15 O]O 2 , this also leads to formation of stable CO. Although a significant increase in Hb‐CO blood concentration after administration of [ 15 O]CO was seen, this increase was small (from 1·00 to 1·13%) and within the range observed in healthy volunteers (0·2–1·4 %) (14). Therefore, the production process described is safe for patients.…”

Time and Sample Site Dependency of the Optimized CO-Rebreathing Method

“…The minute 5 concentration was calculated as the mean of the minute 4 and minute 6 blood samples. 1.39 = Hüfner_s count for the CO-binding capacity of hemoglobin (1.39 mL COIg j1 Hb) (14,24).…”

High affinity of blood for oxygen reduces oxygen uptake in contracting canine gracilis muscle

“…The blood oxygen content in millilitres per decilitre (CO2) was calculated from the relationship (Shimizu, Enoki, Kohzuki, Ohga & Sakata, 1986 (Kohzuki, Enoki, Sakata & Tomita, 1983). Red cell 2,3-diphosphoglycerate was measured enzymatically (Maeda, Chang, Benesch & Benesch, 1971 Figure 1 shows representative results from one of the alternate-perfusion experiments.…”

“…The blood oxygen content in millilitres per decilitre (CO2) was calculated from the relationship (Shimizu, Enoki, Kohzuki, Ohga & Sakata, 1986):…”