Determination of impurities in nucleoside 3′-phosphoramidites by fast atom bombardment mass spectrometry

Pc, Toren; Df, Betsch; Hl, Weith; Jm, Coull

doi:10.1016/0003-2697(86)90411-2

Cited by 6 publications

(1 citation statement)

References 10 publications

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“…The application of nanoelectrospray MS to the analysis of six standard 2 -deoxyribonucleoside phosphoramidites has been reported (Kele et al, 1999). Although FAB and LSI (Ashcroft, 1997) have been effective for MS analysis of phosphoramidites (Toren et al, 1986;Kele et al, 1999), it is not always possible to detect the molecular-related ions (MRIs) of various phosphoramidites by these methods. This limitation prompted the authors to develop the methods described in this unit.…”

Section: Massmentioning

confidence: 99%

Mass Determination of Phosphoramidites

Harusawa

Fujitake

Kurihara

et al. 2006

CP Nucleic Acid Chemistry

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Nucleoside phosphoramidites are the most widely used building blocks in contemporary solid-phase synthesis of oligonucleotides. The accurate molecular weight measurements of such molecules, which are acid-labile compounds, may be easily determined by mass spectrometry using a matrix system, triethanolamine/NaCl, on a liquid secondary ion mass spectrometer (LSIMS) or fast-atom bombardment (FAB) MS equipped with a double-focusing mass spectrometer. The present method rapidly and easily measures the accurate molecular weights of various phosphoramidites as adduct ions [M+Na]+ with an average mass error smaller than 0.4 ppm, allowing determination of the formulas of the phosphoramidites in place of elemental analysis. Further, it was found that intensities of molecular-related ions could be enhanced to the highest degree by adjustment of the molar ratio of phosphoramidite and NaCl, fixing the amount of triethanolamine on LSIMS, making the present method a powerful tool for identification of phosphoramidites by mass spectrometry.

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Section: Massmentioning

confidence: 99%