Determination of MDA Levels in the Plant (Some Salvia L. Taxa Growing in Turkey)

Sarı, Aysel; Kürşat, Murat; Civelek, Åemsettin

doi:10.4172/2157-7609.1000117

Cited by 8 publications

(8 citation statements)

References 16 publications

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“…Malondialdehyde was quantified according to Sari et al (2012) and Zeb and Ullah (2016) , with the following modifications. Seeds were grounded to a fine powdery flour (granulometry from 20 to 200 mm)in a mixer mill type MM200 (Retch, Germany) for 30 s with the frequency set at 1/30.…”

Section: Methodsmentioning

confidence: 99%

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Hydropriming Applied on Fast Germinating Solanum villosum Miller Seeds: Impact on Pre-germinative Metabolism

et al. 2021

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Seed priming can circumvent poor germination rate and uniformity, frequently reported in eggplant (Solanum melongena L.) and its crop wild relatives (CWRs). However, there is still a gap of knowledge on how these treatments impact the pre-germinative metabolism in a genotype- and/or species-dependent manner. The CWR Solanum villosum Miller (hairy nightshade) investigated in this study showed a quite unique profile of fast germination. Although this accelerated germination profile would not apparently require further improvement, we wanted to test whether priming would still be able to impact the pre-germinative metabolism, eventually disclosing the predominant contribution of specific antioxidant components. Hydropriming followed by dry-back resulted in synchronized germination, as revealed by the lowest MGR (Mean Germination Rate) and U (Uncertainty) values, compared to unprimed seeds. No significant changes in ROS (reactive oxygen species) were observed throughout the treatment. Increased tocopherols levels were detected at 2 h of hydropriming whereas, overall, a low lipid peroxidation was evidenced by the malondialdehyde (MDA) assay. Hydropriming resulted in enhanced accumulation of the naturally occurring antioxidant phenolic compounds chlorogenic acid and iso-orientin, found in the dry seeds and ex novo accumulation of rutin. The dynamic changes of the pre-germinative metabolism induced by hydropriming are discussed in view of future applications that might boost the use of eggplant CWRs for breeding, upon upgrade mediated by seed technology.

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Section: Methodsmentioning

confidence: 99%

“…Malondialdehyde was quantified according to Sari et al (2012) and Zeb and Ullah (2016), with the following modifications.…”

Section: Mda Determinationmentioning

confidence: 99%

Hydropriming Applied on Fast Germinating Solanum villosum Miller Seeds: Impact on Pre-germinative Metabolism

et al. 2021

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“…Polyunsaturated lipids are susceptible to ROS-mediated oxidative stress that triggers a chain reaction resulting in end products such as MDA used as marker of lipid peroxidation and membrane damage. MDA levels were quantified according to Sari et al (2012) and Zeb and Ullah, (2016), with the following modifications. Seeds were collected at the indicated timepoints and grinded in a mortar until a powder was obtained.…”

Section: Methodsmentioning

confidence: 99%

How Does the Seed Pre-Germinative Metabolism Fight Against Imbibition Damage? Emerging Roles of Fatty Acid Cohort and Antioxidant Defence

Doria¹,

Pagano²,

Ferreri

et al. 2019

Front. Plant Sci.

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During seed imbibition, lipids are engaged in membrane reorganization while facing free radical-mediated oxidative injury. In the present work, we explored changes in lipid components at different timepoints of imbibition (0.5, 2, 4, 6, and 8 h) in the legume Medicago truncatula, by combining biochemical approaches with targeted lipidomics and untargeted metabolomics. ROS and RNS (reactive oxygen and nitrogen species) accumulation was observed throughout the tested timepoints whereas lipid peroxidation increased at 4 h of imbibition. The seed response to oxidative damage was evidenced by a significant increase in tocopherols starting from 0.5 h of imbibition as well as by the reduction in total thiol content occurring at 2 h of imbibition. Since under physiological conditions, the proper functions of the cell membranes are strongly dependent on the qualitative and quantitative balance of fatty acid residues in phospholipids, the investigation was expanded to the fatty acid cohort of M. truncatula seeds. Total saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), omega(ω)-3 and omega(ω)-6 fatty acids showed fluctuations during seed imbibition. The most remarkable finding was the profile of the ω-3 PUFA docosopentaenoic acid (DPA, 7 cis, 10 cis, 13 cis, 16 cis, and 19 cis-22:5) that showed a peak (up to 1.0% of the total fatty acid content) at 0.5 and 8 h of imbibition, concomitant with the peaks observed in tocopherol levels. It is possible that the observed changes in DPA alter the physical properties of membranes, as reported in animal cells, triggering signaling pathways relevant for the cell defense against oxidative injury. Furthermore, the content and balance between tocopherols and PUFAs is regarded as a determinant of storage stability. No enhancement in trans-fatty acids occurred throughout imbibition, suggesting for a proper antioxidant response carried by the seed. Fatty acids profiles were integrated with data from untargeted metabolomics showing changes in lipid sub-pathways, among which fatty acid amide, lyso-phospholipids, and phospholipid metabolism. The emerging lipid profiles and dynamics are discussed in view of the overall imbibition damage generated during M. truncatula seed imbibition.

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“…As important medical plants since ancient times, the leaves, flowers, shoot tips, and partial stems of Salvia species are all utilized. In folk medicine, sage leaves are valued for the treatment of many disorders and have been used for their soothing properties, for pain relief and prevention of colds and coughs, for lowering blood pressure, and for sweat-breaking; they have been used as expectorants, disinfectants, and muscle pain relievers, and as a part of midwifery practice (Sarı et al, 2012;Bayram et al, 2016). Consumption of these species as herbal teas is quite common, as well.…”

mentioning

confidence: 99%

“…it is used for the treatment of wounds and various skin diseases. A decoction prepared with aerial parts of this species is used to prevent blood cancer (Baytop, 1999;Poyraz and Koca, 2006;Sarı et al, 2012). In addition, it is reported that this plant is consumed as a strong tea in Iran (Hatipoğlu et al, 2016) and has several biological activities (antimicrobial, antioxidant, alpha-amylase, GSH-Px, antinociceptive, antiinflammatory, peroxidase, polyphenol oxidase, and acetylcholinesterase activities) (Alizadeh, 2013;Jeshvaghani et al, 2015).…”

mentioning

confidence: 99%

Determination of essential oil components, mineral matter, and heavy metal content of Salviavirgata Jacq. grown in culture conditions

Şenkal¹,

Uskutoğlu²,

Cesur³

et al. 2019

Turk J Agric For

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Introduction Most members of the family Lamiaceae have considerable importance in areas such as medicine, food, cosmetics, and perfumery, as they are rich in essential oils, aromatic oils, and secondary metabolites. Sage, a member of this family, is the generic name for species in the genus Salvia, which has approximately 900 species worldwide (Dweck, 2000). There are 99 species of the genus Salvia L. in Turkey; 51 of these species are endemic. Turkey is one of the important gene centers for Salvia species (Güner et al., 2012). These species include annual, perennial, and biennial plants in bush form. As important medical plants since ancient times, the leaves, flowers, shoot tips, and partial stems of Salvia species are all utilized. In folk medicine, sage leaves are valued for the treatment of many disorders and have been used for their soothing properties, for pain relief and prevention of colds and coughs, for lowering blood pressure, and for sweat-breaking; they have been used as expectorants, disinfectants, and muscle pain relievers, and as a part of midwifery practice (Sarı et al., 2012; Bayram et al., 2016). Consumption of these species as herbal teas is quite common, as well. Salvia species appeal to a wide range of consumer groups due to their characteristics, and because they are used in the food, pharmaceutical, and chemical industries their market potential is quite high. The S. virgata species belonging to the genus Salvia is a perennial and herbaceous plant that can grow up to 30-100 cm high with an erect and highly branched structure. The leaves are simple, usually scattered on the body, sometimes collected on the base. The flower is a compound cluster. The petals are violet, blue, or mauve, rarely white, and it favors a wide variety of habitats such as shrubland, forests, meadows, low fields, limestone and volcanic rocks, and roadsides (

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Determination of MDA Levels in the Plant (Some Salvia L. Taxa Growing in Turkey)

Cited by 8 publications

References 16 publications

Hydropriming Applied on Fast Germinating Solanum villosum Miller Seeds: Impact on Pre-germinative Metabolism

Hydropriming Applied on Fast Germinating Solanum villosum Miller Seeds: Impact on Pre-germinative Metabolism

How Does the Seed Pre-Germinative Metabolism Fight Against Imbibition Damage? Emerging Roles of Fatty Acid Cohort and Antioxidant Defence

Determination of essential oil components, mineral matter, and heavy metal content of Salviavirgata Jacq. grown in culture conditions

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