Determination of morphine in the plasma of addicts in using Zeolite Y extraction following high-performance liquid chromatography

Ghazi‐Khansari, Mahmoud; Zendehdel, Rezvan; Pirali-Hamedani, Morteza; Amini, Mohammad K.

doi:10.1016/j.cccn.2005.07.002

Cited by 26 publications

(11 citation statements)

References 16 publications

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“…Using morphine pellets is a standard method for continuously administering morphine to prevent cycles of withdrawal in mice, and it produces brain drug levels considered to be similar to blood/tissue levels achieved in humans who are tolerant and dependent on opiates (Ozaita et al, 1998;Ghazi-Khansari et al, 2006), and therapeutic levels seen in patients maintained on chronic opiates/ opiate pumps for intractable pain (Balch and Trescot, 2010).…”

Section: Placebo or Chronic Morphine Administrationmentioning

confidence: 99%

Morphine Tolerance and Physical Dependence Are Altered in Conditional HIV-1 Tat Transgenic Mice

Fitting

Stevens

Khan

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

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Despite considerable evidence that chronic opiate use selectively affects the pathophysiologic consequences of human immunodeficiency virus type 1 (HIV-1) infection in the nervous system, few studies have examined whether neuro-acquired immune deficiency syndrome (neuroAIDS) might intrinsically alter the pharmacologic responses to chronic opiate exposure. This is an important matter because HIV-1 and opiate abuse are interrelated epidemics, and HIV-1 patients are often prescribed opiates as a treatment of HIV-1-related neuropathic pain. Tolerance and physical dependence are inevitable consequences of frequent and repeated administration of morphine. In the present study, mice expressing HIV-1 Tat in a doxycycline (DOX)-inducible manner [Tat(1)], their Tat(2) controls, and control C57BL/6 mice were chronically exposed to placebo or 75-mg morphine pellets to explore the effects of Tat induction on morphine tolerance and dependence. Antinociceptive tolerance and locomotor activity tolerance were assessed using tail-flick and locomotor activity assays, respectively, and physical dependence was measured with the platform-jumping assay and recording of other withdrawal signs. We found that Tat(1) mice treated with DOX [Tat(1)/DOX] developed an increased tolerance in the tail-flick assay compared with control Tat(2)/DOX and/or C57/DOX mice. Equivalent tolerance was developed in all mice when assessed by locomotor activity. Further, Tat(1)/DOX mice expressed reduced levels of physical dependence to chronic morphine exposure after a 1-mg/kg naloxone challenge compared with control Tat(2)/DOX and/or C57/DOX mice. Assuming the results seen in Tat transgenic mice can be generalized to neuroAIDS, our findings suggest that HIV-1-infected individuals may display heightened analgesic tolerance to similar doses of opiates compared with uninfected individuals and show fewer symptoms of physical dependence.

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Section: Placebo or Chronic Morphine Administrationmentioning

confidence: 99%

Morphine Tolerance and Physical Dependence Are Altered in Conditional HIV-1 Tat Transgenic Mice

Fitting

Stevens

Khan

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

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“…Mor for brain and blood dialysate at S/N ratio of 3 were 0.4 ng/ml (2.7 fmol on column) and 0.6 ng/ml (3.8 fmol on column), respectively. The proposed method could improve the sensitivity of Mor by more than 20 times compared to HPLC-DAD method [7,8] and native FL method [13][14][15]. Furthermore, it was comparable to those of HPLC-ECD [10] and -MS/MS method [20] published recently for determination of Mor in microdialysate.…”

Section: Methods Validationmentioning

confidence: 48%

“…Quite recently, HPLC methods combined with tandem MS/MS for very low-level quantification of Mor in biological samples have been described with sub-nanogram per milliliter of quantitation limit [17][18][19][20]. In the HPLC-FL detection methods, either native FL of Mor [13][14][15] or FL of labeled Mor [16] is used. The native FL detection method with 10-50 ng/ml of detection limits is not enough sensitive to detect very small amount of Mor.…”

Section: Introductionmentioning

confidence: 99%

“…For the determination of Mor, high performance liquid chromatography (HPLC) methods with UV or DAD detection [7,8], electrochemical detection (ECD) [9,10], mass spectrometry (MS) detection [11,12] and fluorescence detection (FL) [13][14][15][16] are widely used. Quite recently, HPLC methods combined with tandem MS/MS for very low-level quantification of Mor in biological samples have been described with sub-nanogram per milliliter of quantitation limit [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac

et al. 2008

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“…Various analytical methods have been developed for determining morphine and its major metabolites. Typically, the common analytical techniques currently used for monitoring morphine's concentration include GC, 3,4 HPLC, [5][6][7][8][9][10] and their combination with other detection methods. Moreover, there are some other methods can be used as well like capillary electrophoresis, 11,12 chemiluminescence, 13 voltammetric, 14,15 electrochemical, 16 and spectrofluorometric.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometry Method for the Determination of Morphine, Nalbuphine and Naltrexone Drugs in Bulk and Pharmaceutical Formulations

El‐Didamony

Saad

Saleem

2013

J. Chil. Chem. Soc.

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A novel study describes the development and validation of a selective and simple kinetic spectrophotometric method for the determination of some analgesic drugs namely, morphine (MOF), nalbuphine (NAB) and naltrexone (NAT) in bulk and in pharmaceutical formulations. This method was based on the reaction of the drugs under studying with alkaline KMnO 4 to form a water-soluble bluish green colored product with a maximum absorbance at 605 nm. Although, the determination of MOF, NAB and NAT drugs by rate constant, fixed-concentration and fixed time methods were feasible with the calibration equations obtained, the fixed time method has been found to be more applicable. The fixed-time method is adopted for constructing the calibration curves, which were found to be linear over the concentration ranges of 4 -18 mg mL -1 MOF, 2 -20 mg mL -1 NAB and 2 -16 mg mL -1 NAT. Different experimental parameters which can affect the development and stability of the color were carefully studied and optimized. The fixed time method of 20 min was further applied to pharmaceutical formulations of each drug and the percentage recoveries were 99.85±0.049 to 102.27±0.024. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.

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Determination of morphine in the plasma of addicts in using Zeolite Y extraction following high-performance liquid chromatography

Cited by 26 publications

References 16 publications

Morphine Tolerance and Physical Dependence Are Altered in Conditional HIV-1 Tat Transgenic Mice

Morphine Tolerance and Physical Dependence Are Altered in Conditional HIV-1 Tat Transgenic Mice

Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac

Kinetic Spectrophotometry Method for the Determination of Morphine, Nalbuphine and Naltrexone Drugs in Bulk and Pharmaceutical Formulations

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