Determination of naproxen in human urine by solid-phase microextraction coupled to liquid chromatography

Aresta, Antonella; Palmisano, Francesco; Zambonin, Carlo

doi:10.1016/j.jpba.2005.04.017

Cited by 101 publications

(53 citation statements)

References 22 publications

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“…For SPME and high-performance liquid chromatography (HPLC) coupling, an organic solvent or the mobile phase is used to desorb the analytes from the fiber using an interface or a off-line desorption (Popp et al, 2000;Sarrión et al, 2002). However, some authors have noted a peak broadening and tailing, particularly when dynamic desorption with mobile phase in a HPLC interface is used (Aresta et al, 2005). In off-line desorption, the analytes could be not totally desorbed or result a broad chromatographic peak (González-Toledo et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Coupling of solid‐phase microextraction with micellar desorption and high performance liquid chromatography for the determination of pharmaceutical residues in environmental liquid samples

Torres-Padrón

Sosa‐Ferrera

Rodríguez

2009

Biomedical Chromatography

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A residue analytical method combining solid-phase microextraction (SPME) with external micellar desorption (MD) and high-performance liquid chromatography with diode array detector (HPLC-DAD) has been developed and validated for the simultaneous determination of six pharmaceutical compounds, belonging to various therapeutic categories in water samples. Target compounds include antiinflamatory drugs (ibuprofen, ketoprofen and naproxen), an analgesic (phenazone), a lipid regulator (bezafibrate) and an antiepileptic (carbamazepine). A detailed study of the experimental conditions of extraction and desorption with different surfactants was performed in order to obtain the best results during instrumental analysis. Of the different fibers and surfactants investigated, 65 microm polydimethysiloxane-divinilbenzene (PDMS-DVB) fiber and polyoxyethylene 10 lauryl ether (POLE) and polyoxyethylene 6 lauryl ether (C(12)E(6)) as desorbing agents produced the optimal response to pharmaceutical residues. Recoveries obtained were generally higher than 80% and the variability of the method was below 16% for all compounds in both surfactants. Method detection limits were 0.05-12 ng mL(-1) for POLE and 0.1-5 ng mL(-1) for C(12)E(6). The developed method was compared using external desorption with organic solvent and it was successfully applied to the determination of these pharmaceutical compounds in water samples from different origin. Solid-phase microextraction with micellar desorption (SPME-MD) represents a new approach for the extraction of different pharmaceutical compounds in natural waters because it combines shorter handling time, better efficiency, safety and more environmentally friendly process than the traditional methods.

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Section: Introductionmentioning

confidence: 99%

Coupling of solid‐phase microextraction with micellar desorption and high performance liquid chromatography for the determination of pharmaceutical residues in environmental liquid samples

Torres-Padrón

Sosa‐Ferrera

Rodríguez

2009

Biomedical Chromatography

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“…Different techniques have been reported for the determination of NAP i.e. chip-based immunoaffinity capillary electrophoresis [20], HPLC [21], HPTLC [22], LC-MS/MS [23] and GC/MS [24]. For the determination of MEL, spectrophotometric [25], Voltammetric [26], fluorimetric [27], electrochemical [28], HPLC [29] and capillary electrophoresis [30] are reported.…”

Section: Introductionmentioning

confidence: 99%

An Ultra-Sensitive and Selective LC-UV Method for the Simultaneous Determination of Pravastatin, Diltiazem, Naproxen Sodium and Meloxicam in API, Pharmaceutical Formulations and Human Serum

Sultana¹

2013

AJAC

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Abstract:The aim of the present work was to develop a sensitive liquid chromatographic method for the quantitation of pravastatin and diltiazem along with naproxen and meloxicam using ultraviolet detector. The separation of components was achieved on Purospher Star, C 18 (5 µm, 25 x 0.46 cm) column using methanol-water (80:20 v/v) as mobile phase and pH adjusted 3.4 with 85% o-phosphoric acid. Related parameters that may influence the enrichment efficiency of speration of drugs such as the kind and volume of elute, sample flow rate, sample pH, and volume of the drug samples were investigated. Detection was performed at ambient temperature at 220 nm by pumping the mobile phase at the flow rate 1.0 mL min -1 . The experimental results indicated a good linearity (R 2 > 0.9947) over the concentration range of 0.5-20 µg mL -1 for pravastatin, naproxen and meloxicam and 0.75-24 µg mL -1 for diltiazem. The method was compared by programming the detector adjusting the wavelength with time to match the individual analyte's chromophore which enhanced sensitivity with linear range 0.25-8.0, 0.5-16, 0.4-12 and 0.2-4.0 µg mL -1 for pravastatin, diltiazem, naproxen and meloxicam respectively. The LOD values shifted down from 33, 70, 50 and 80 ng mL -1 to 15, 42, 20 and 10 ng mL -1 for pravastatin, diltiazem, naproxen and meloxicam respectively. Validation of the method showed good precision and accuracy for the proposed method. All the results indicated that this procedure could allow the simultaneous determination of these four compounds in API, pharmaceutical formulations and serum at trace levels. The method can be successfully applied for the determination of these drugs in human serum, clinical laboratories and in pharmaceutical formulations without diode array detector and without interference of excipients or endogenous components of serum.

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“…Naproxen is rapidly and completely absorbed after oral administration, and it is the predominant species in serum, with a therapeutic range of 30 to 90 μg mL -1 4 . Naproxen has been determined by several analytical methods like: HPLC [5][6][7] , HPTLC 8 , LC-MS/MS 9,10 , capillary electrophoresis 11,12 , fluorimetric methods 13 , chemiluminescence [14][15][16] , voltammetry 17,18 , solid-phase microextraction coupled with liquid chromatography 19 and spectrophotometry [20][21][22] . Although the HPLC methods are highly sensitive and specific, but they are most expensive.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of Naproxen as ion-pair with bromophenol blue in bulk, pharmaceutical preparation and human serum samples

Keyhanian¹,

Alizadeh²,

Shojaie³

2013

10.5267/j.ccl

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A highly sensitive, accurate and simple spectrophotometric method was established for determination of naproxen (NAP). The method involved ion-pair complex formation between naproxen and bromophenol blue (BPB). The colored product was measured at 432 nm. The reaction conditions were optimized. The absorbance was found to increase linearly with increase in concentration of NAP which was corroborated by correlation coefficient value. Beer's law was obeyed in the concentration range of 1-110 µg mL -1 with molar absorptivity of 9.756×10 3 L mol -1 cm -1. The limits of detection (LOD) and quantitation (LOQ) for the proposed method are 0.292 and 0.973 µg mL -1 , respectively. Recovery of the method was carried out by standard addition method. Recovery studies and statistical data proved the accuracy, reproducibility and the precision of the proposed method. The common excipients did not interfere in this analysis. Hence the method is useful for routine estimation of naproxen in pharmaceutical formulation and human serum samples.

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Determination of naproxen in human urine by solid-phase microextraction coupled to liquid chromatography

Cited by 101 publications

References 22 publications

Coupling of solid‐phase microextraction with micellar desorption and high performance liquid chromatography for the determination of pharmaceutical residues in environmental liquid samples

Coupling of solid‐phase microextraction with micellar desorption and high performance liquid chromatography for the determination of pharmaceutical residues in environmental liquid samples

An Ultra-Sensitive and Selective LC-UV Method for the Simultaneous Determination of Pravastatin, Diltiazem, Naproxen Sodium and Meloxicam in API, Pharmaceutical Formulations and Human Serum

Spectrophotometric determination of Naproxen as ion-pair with bromophenol blue in bulk, pharmaceutical preparation and human serum samples

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