Determination of Naturally Occurring Formaldehyde Levels in Sap and Wood Tissue of Maple Trees Using Gas Chromatgraphy/Mass Spectrometry

Lagacé, Luc; Gaudy, Réjean; Perez-Locas, Carolina; Sadiki, Mustapha

doi:10.5740/jaoacint.10-443

Cited by 3 publications

(1 citation statement)

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“…The potent cytotoxin formaldehyde can be formed in nature for example by incomplete combustion of organic material (Peters et al, 2012) or in plant biomass (Lagacé et al, 2012) and can enter the soil, the natural habitat of C. glutamicum, by rainfall or degradation of plant material, respectively. Here, we could show that the formaldehyde detoxification system of C. glutamicum encoded by fadH and ald is crucial for the tolerance of this external stress by free formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

Formaldehyde degradation in Corynebacterium glutamicum involves acetaldehyde dehydrogenase and mycothiol-dependent formaldehyde dehydrogenase

2013

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Corynebacterium glutamicum, a Gram-positive soil bacterium belonging to the actinomycetes, is able to degrade formaldehyde but the enzyme(s) involved in this detoxification process were not known. Acetaldehyde dehydrogenase Ald, which is essential for ethanol utilization, and FadH, characterized here as NAD-linked mycothiol-dependent formaldehyde dehydrogenase, were shown to be responsible for formaldehyde oxidation since a mutant lacking ald and fadH could not oxidize formaldehyde resulting in the inability to grow when formaldehyde was added to the medium. Moreover, C. glutamicum DaldDfadH did not grow with vanillate, a carbon source giving rise to intracellular formaldehyde. FadH from C. glutamicum was purified from recombinant Escherichia coli and shown to be active as a homotetramer. Mycothiol-dependent formaldehyde oxidation revealed K m values of 0.6 mM for mycothiol and 4.3 mM for formaldehyde and a V max of 7.7 U mg "1. FadH from C. glutamicum also possesses zinc-dependent, but mycothiolindependent alcohol dehydrogenase activity with a preference for short chain primary alcohols such as ethanol (K m 5330 mM, V max 59.6 U mg "1 ), 1-propanol (K m 5150 mM, V max 55 U mg "1 ) and 1-butanol (K m 550 mM, V max 50.8 U mg "1). Formaldehyde detoxification system by Ald and mycothiol-dependent FadH is essential for tolerance of C. glutamicum to external stress by free formaldehyde in its habitat and for growth with natural substrates like vanillate, which are metabolized with concomitant release of formaldehyde.

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Section: Discussionmentioning

confidence: 99%

Formaldehyde degradation in Corynebacterium glutamicum involves acetaldehyde dehydrogenase and mycothiol-dependent formaldehyde dehydrogenase

2013

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Carbon-13 nuclear magnetic resonance analysis of formaldehyde free preservatives

Das¹,

Dumais

Basiaga

et al. 2013

Acta Histochemica

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Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde

Ghelli

Bellisario

Squillacioti

et al. 2021

J Occup Med Toxicol

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Background Many workers are exposed to wood dust (WD) and formaldehyde (FA), whose carcinogenic activity is supposed to be oxidative stress-mediated. This study aims to assess to what extent the occupational exposure to WD and FA, albeit within regulatory limits, could result in OS induction in a woodworkers’ population. Methods The sample population consisted of 127 woodworkers from 4 factories and 111 unexposed controls. Individual exposure was assessed by personal air-samplers. Each participant enrolled in the study filled out a questionnaire and provided a urinary sample to quantify OS biomarkers, namely 15-F2t-IsoProstane (15-F2t-IsoP) and 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dGuo). The main confounding factor for OS, i.e. tobacco smoking exposure, was assessed by measuring cotinine in urine samples. Results Woodworkers were exposed to significantly higher amounts of WD and FA as compared to controls (p < 0.001). Among OS biomarkers, 15-F2t-IsoP showed statistically significant higher values in woodworkers compared to controls (p = 0.004). A significant, positive correlation was observed between 15-F2t-IsoP and 8-oxo-dGuo (p = 0.005), cotinine (p = 0.05), FA (p < 0.001) and WD (p = 0.01); 8-oxo-dGuo was significantly correlated with cotinine (p = 0.001) and WD (p = 0.004). In addition, WD and FA were significantly correlated each other (p < 0.001). Conclusions The study confirms that WD and FA may induce OS in woodworkers, and highlights that even the compliance with occupational exposure limits can result in measurable biological outcomes.

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Determination of Naturally Occurring Formaldehyde Levels in Sap and Wood Tissue of Maple Trees Using Gas Chromatgraphy/Mass Spectrometry

Cited by 3 publications

References 0 publications

Formaldehyde degradation in Corynebacterium glutamicum involves acetaldehyde dehydrogenase and mycothiol-dependent formaldehyde dehydrogenase

Formaldehyde degradation in Corynebacterium glutamicum involves acetaldehyde dehydrogenase and mycothiol-dependent formaldehyde dehydrogenase

Carbon-13 nuclear magnetic resonance analysis of formaldehyde free preservatives

Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde

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