Determination of Neotame by High-Performance Capillary Electrophoresis Using ß-cyclodextrin as a Chiral Selector

Abstract

An electrokinetic chromatographic method was developed for the chiral separation of neotame, a new high intensity artificial sweetener, using a chiral separating agent heptakis 2,3,6-tri-o-methylbetacyclodextrin. The purpose of this study was to better understand diastereomer-resolution interactions between neotame and the chiral separating agent. Molecular docking studies were performed to elucidate the mechanism of the separation. The optimum conditions were 50 mM phosphate buffer, pH 5.5, applied voltage 20 kV, cassette temperature of 30 C, and a 4 s sample injection time. The calibration curve showed good linearity (r 2 > 0.99) with recoveries for both diastereomers, ranging from 95.66-99.00% and the limits of detection for L,L-neotame and D,D-neotame were 0.01857 and 0.08214 mM, respectively. The developed method showed analytical precision with relative standard deviations (n ¼ 5) of 1.20% and 1.17% with respect to migration time and peak area, respectively. A large difference in the interaction energies observed between the diastereomers represents a significant differentiation. The results showed that both electrostatic and hydrophobic interactions played a significant role in stabilizing their inclusion complexes and consequently supported the elution order based on their differential stabilities.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…and the LOD was 15.52 μM. Another chiral CE method was reported for the chiral separation of neotame . Neotame is an artificial sweetener with two chiral centers in its structure, hence, it can form four diastereomers being its sweetness attributed to the presence of L,L‐diastereomer.…”

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

“…Despite the successful chiral separation, the particular method provided poor reproducibility, probably due to the instability of the drug complexes being formed. TM‐β‐CD was also used for the chiral separation of neotame ( d , d ‐ and l,l ‐) and lercanidipine .…”

Electrochemical Enzymatic Biosensing of Neotame Supported by Computational Methods

“…The European Food Safety Authority also estimated possible daily intakes to be below the average daily intake (ADI) levels and that ingested neotame can form phenylalanine, not notable to people suffering from phenylketonuria. Extensive literature studies for the determination of neotame, revealed various techniques including High Performance Liquid Chromatography [3] and Capillary Electrophoresis [4,5] has been reported. Satisfactory results were reported for the sensitivity, selectivity and detection limits, but required a large number of standard operating procedures (SOPs) that are time consuming.…”