Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC

Khan, Abdul Rafiq; Khan, Khalid M.; Perveen, Shahnaz; Butt, Naeem M.

doi:10.1016/s0731-7085(02)00039-0

Cited by 15 publications

(6 citation statements)

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“…As was described in introduction part of this work, several methods including high-performance liquid chromatography (HPLC) and also gas chromatography (GC) were described for the analysis of nicotinamide and its derivatives in biological samples, food products, and also in selected pharmaceutical formulations. Based on the analysis of data presented in this work and those obtained by use of other chromatographic techniques (e.g., HPLC and GC), it could be observed that the limit of quantification for nicotinamide by use of HPLC-UV was found to be in μ g/mL (e.g., from 11 to 34 μ g/mL) [ 32 ]. A gas chromatographic method enabled determination of nicotinamide content in the range from mg/mL to μ g/mL [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Dołowy

Pyka

2015

Journal of Analytical Methods in Chemistry

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This research study describes the applicability of silica gel 60 RPW18F254 plates for the development and validation of new, simple, economic, accurate, and precise RPHPTLC-densitometric method suitable for the quantification of nicotinamide (as Vitamin PP) in three marketed preparations. The mobile phase used was methanol-water in volume composition 3 : 7. Detection wavelength was 200 nm. The proposed method was validated according to ICH guidelines and also based on Ferenczi-Fodor and Konieczka reports. Results were found to be linear over a range of 1.00 to 2.00 μg/spot. Limit of detection (LOD) and limit of quantification (LOQ) were 0.15 μg/spot and 0.45 μg/spot, respectively. The percent content of nicotinamide in the investigated preparations was found to be 99.2% (Product 1), 99.3% (Product 2), and 99.4% (Product 3). Developed method is accurate and precise (CV < 3%) and may be successfully applied for the quality control of pharmaceutical formulations containing nicotinamide in the presence of its derivatives, such as N,N-diethylnicotinamide, N-methylnicotinamide, and nicotinic acid.

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Section: Resultsmentioning

confidence: 99%

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Dołowy

Pyka

2015

Journal of Analytical Methods in Chemistry

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“…Nicotinamide was analyzed by HPLC according to [16]. Separation was performed on a Bondapak C18 column with a mixture of methanol and buffer (75:25, v/v) of 0.05 M potassium dihydrogen phosphate having pH 3.6 at flow rate of 1.5 ml min -1 and UV detection at 254 nm.…”

Section: Hplc Analysismentioning

confidence: 99%

Combined Patch Containing Salicylic Acid and Nicotinamide: Role of Drug Interaction

Padula

Ferretti²,

Nicoli³

2010

CDD

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The aim of the present study was to formulate a combined patch containing salicylic (SA) acid and nicotinamide (NA), useful for the treatment of mild acne, and to verify their mutual effect on drug permeation and skin retention. The performance of the patch was tested in vitro in permeation experiments using pig ear skin as barrier. To better understand the data obtained from the film, permeation from solutions and isopropyl myristate/water partition coefficient were also determined. The results obtained in the present work suggest a mutual influence of NA and SA on their permeation across the skin from an innovative transdermal film. The partition coefficient obtained when the two molecules were simultaneously present was typically lower than the respective value obtained with NA and SA alone.

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“…Although the retention time of methoxsalen was less than 4.5 min, the method does not involve the forced degradation studies which make it unsuitable for stability monitoring. The analytical techniques that had been reported for p-aminobenzoic acid included determination by high performance liquid chromatography [8][9], spectrophotometry [10][11] and titrimetry [12]. Kumar et al [13] have analysed p-aminobenzoic acid in multivitamin dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for simultaneous determination of methoxsalen and p-aminobenzoic acid in binary combination

John¹,

Azeem²,

Ashfaq³

et al. 2015

Bull. Chem. Soc. Eth.

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ABSTRACT. An accurate, simple and specific reverse phase LC procedure is established and validated for simultaneous estimation of methoxsalen and p-aminobenzoic acid in pharmaceutical formulated products and human serum. Chromatographic separation among methoxsalen, p-aminobenzoic acid and their degradation products have been achieved in less than 5 min with Hypersil-ODS column (250 mm x 4.6 mm, 5 µm), using acetonitrile and 1.28 mM phosphate buffer as mobile phase (60:40 v/v). Flow rate of the mobile phase was set as 1.5 mL min -1 and detection of all the analytes was carried out by diode-array detector at 254 nm. The developed method was validated according to ICH guidelines by performing its linearity, accuracy, precision, specificity, robustness and LOD/LOQ values. Response was linear and proportional to the concentrations (24-48 µg mL -1 ) for p-aminobenzoic acid and (9-18 µg mL -1 ) for methoxsalen. The LOD was 2.3 ng mL -1 for p-aminobenzoic acid and 6 ng mL -1 for methoxsalen whereas LOQ was 7.8 ng mL -1 for p-aminobenzoic acid and 20.4 ng mL -1 for methoxsalen. The developed method efficiently separated the principal peaks from degradation products and therefore can be applied successfully for concurrent analysis of methoxsalen and p-aminobenzoic acid in pharmaceutical dosage form, human serum and product stability studies.

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Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC

Cited by 15 publications

References 1 publication

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Combined Patch Containing Salicylic Acid and Nicotinamide: Role of Drug Interaction

Stability-indicating RP-HPLC method for simultaneous determination of methoxsalen and p-aminobenzoic acid in binary combination

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Determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation by LC

Cited by 15 publications

References 1 publication

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF254for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF254for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Combined Patch Containing Salicylic Acid and Nicotinamide: Role of Drug Interaction

Stability-indicating RP-HPLC method for simultaneous determination of methoxsalen and p-aminobenzoic acid in binary combination

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Product

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Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations

Validation of an RPHPTLC-Densitometric Method Using Silica Gel 60 RP18WF₂₅₄for Simultaneous Determination of Nicotinamide in Selected Pharmaceutical Formulations