Determination of Nitrofuran Metabolites in Muscular Tissue by High-Performance Liquid Chromatography with Mass Spectrometric Detection

Куликовский, А. Г.; Горлов, И.Ф.; Slozhenkina, M I; Вострикова, Н. Л.; Ivankin, Andrey; Кузнецова, О. А.

doi:10.1134/s106193481907013x

Cited by 20 publications

(6 citation statements)

References 14 publications

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“…HPLC-MS is considered the most efficient technique among these methods due to its reliability, sensitivity, and selectivity [24,26,28]. Although many HPLC-MS methods have been reported in recent years to detect nitrofuran derivatives in complex matrix foods (e.g., honey [26,[30][31][32][33][34][35], milk [34][35][36][37][38], meat [34,35,[39][40][41][42][43], seafood [44,45], and eggs [34,46,47]), there are still problems that need to be solved more or less. For example, a sample clean-up step, usually SPE, is typically required to eliminate matrix effects.…”

Section: Introductionmentioning

confidence: 99%

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

Gong

et al. 2022

Journal of Chemistry

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In this study, a rough, cheap, easy-made wooden-tip-based solid-phase microextraction (SPME) probe was first developed for simultaneous determination of 4 nitrofuran metabolite derivatives in complex food matrices via LC-MS/MS. A simple dip-coating method was used to coat wooden tips with biocompatible polyacrylonitrile (PAN) and N-vinylpyrrolidone-co-divinylbenzene, also known as HLB particles, which served as the extractive substrate in the proposed device. Compared with the traditional solid-phase extraction (SPE) method, the proposed device shortens sample clean-up time, reduces solvent consumption, and decreases testing costs. In addition, the main parameters affecting the SPME procedure efficiency were investigated in detail and the optimal conditions were found. The method was validated using three different food matrixes (pork, croaker, and honey) by spiking with the four metabolites at 0.5, 1.0, and 5.0 μg/kg, as well as their internal standards. The average recovery of all nitrofuran metabolite derivatives ranges from 97.4–109.5% (pork), 87.5–112.7% (croaker), and 98.6–109.0% (honey). Relative standard deviations were all <10% for intraday and interday precision. The values of limit of detection and limit of quantification were, respectively, ranging from 0.011 to 0.123 and 0.033 to 0.369 μg/kg (pork), 0.009 to 0.112 and 0.027 to 0.339 μg/kg (croaker), and 0.010 to 0.131 and 0.030 to 0.293 μg/kg (honey). The presented method was applied to the analysis of real positive samples.

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Section: Introductionmentioning

confidence: 99%

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

Gong

et al. 2022

Journal of Chemistry

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“…Indeed, various methods have been established for detecting NFZ and/or SEM (the metabolite of NFA) in animal-derived foods, such as high-performance liquid chromatography-ultraviolet (HPLC-UV) [ 10 ], HPLC with fluorescence (HPLC-FLD) [ 11 ], and HPLC-tandem mass spectrometry (HPLC-MS/MS) [ 12 , 13 , 14 , 15 , 16 ]. However, the above instrumental methods require professional knowledge of operators and costly instruments, and they are unsuitable for on-site detection, which limits their use.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay

Jia

Zhou

et al. 2021

Foods

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Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 μg kg−1 for SEM, respectively, with the linear range of 0.08–0.41 μg L−1, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation <15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS).

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“… 4 − 6 These NF metabolite compounds have been proven to pose potential risk to human health because of their carcinogenic, teratogenic, and mutagenic potency. 5 , 7 Based on food safety needs, the use of NF drugs was banned by the European Union during the cultivation of animal products. 8 , 9 Since then, many countries have followed the suit, such as the United States, China, and Japan.…”

Section: Introductionmentioning

confidence: 99%

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

et al. 2020

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Nitrofuran (NF) is a class of broad-spectrum antibiotics that are used illegally in animal feeding. NF and its metabolites have proven to pose potential risk to human health. To address the current analytical needs to quantify low levels of NF metabolites in animal foods, a sensitive method was developed for simultaneous detection of four NF metabolites in fish products by an ultraperformance liquid chromatography-diode array detector (UPLC-DAD). With 2-nitrobenzaldehyde (2-NBA) as the derivatizing reagent, the metabolites were hydrolyzed and derivatized under the assistance of thermostatic ultrasound. Compared with the current detection methods, the time of the derivatization reaction has been shortened from 16 to 2 h. The relative coefficient of four NF metabolite derivatives reached more than 0.998, with excellent linear relationship. The limits of detection (LODs) and limits of quantification (LOQs) of six repeated determinations reached 0.25–0.33 and 0.80–1.10 μg/kg, respectively. For all four NF metabolites, the limit of detection of the method was below the minimum required performance limit (MRPL) of 1.0 μg/kg, which makes it compatible with the EU requirements. The recoveries ranging from 89.8 to 101.9% with relative standard deviation below 6.5% were obtained for all of the NF metabolites. What’s more, this method was successfully applied for the determination of four NF metabolites in the fish products. As a promising approach, this method could also be extended for the quantitation of NF metabolites in aquaculture and poultry products.

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Determination of Nitrofuran Metabolites in Muscular Tissue by High-Performance Liquid Chromatography with Mass Spectrometric Detection

Cited by 20 publications

References 14 publications

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

Determination of Nitrofuran Metabolites in Complex Food Matrices Using a Rough, Cheap, Easy-Made Wooden-Tip-Based Solid-Phase Microextraction Probe and LC-MS/MS

Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

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